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Plasmid#13528DepositorInsertB. subtilis DEAD-box helicase YxiN first two domains
UseTagsintein-chitin binding domainExpressionBacterialMutationdeleted amino acid residues 369-479PromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
BS.YxiN.Twin1.a404-479.wt
Plasmid#13529DepositorInsertB. subtilis DEAD-box helicase YxiN specific RNA binding domain
UseTagsintein-chitin binding domainExpressionBacterialMutationdelete amino acid residues 1-403PromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI Probe
Plasmid#211507PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol (PI) from bacteriaDepositorInsertBcPI-PLC-ANH
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJC175e
Plasmid#79219PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPPSP gIII, luxAB
UseTagsExpressionBacterialMutationPromoterPPSPAvailable sinceApril 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT130_GalL_nCDA1-NGBE3
Plasmid#145094PurposeExpressing base editor nCDA1-NGBE3 in yeast cellsDepositorInsertnCDA1-NGBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1…PromoterGalLAvailable sinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcpBpaRS_v2
Plasmid#196485PurposeTo express benzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into benzoylphenylalanine.DepositorInsertbenzoylphenylalanine-specific variant of E. coli TyrRS (pBpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB061i
Plasmid#79226PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB061i3
Plasmid#79230PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcpAzpaRS_v2
Plasmid#196483PurposeTo express azidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS) and its cognate amber suppressor tRNA to allow UAG codon to be translated into azidophenylalanine.DepositorInsertazidophenylalanine-specific variant of E. coli TyrRS (pAzpaRS), amber suppressor tRNA
UseTagsExpressionMammalianMutationPromoterSV40(synthetase), Pol III tRNA promoter (tRNA), C…Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB078d3
Plasmid#79203PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
eGFP-CBP
Plasmid#169211PurposeExpression of eGFP-CBP protein for chitin visualizationDepositorInsertChitinase A1 Chitin Binding Domain
UseTags6xHis, Mxe Gyr Intein, and eGFPExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB107a
Plasmid#79218PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) gIII, luxAB; Ppro1 434cI
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB045a
Plasmid#79224PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ rpoZ-HA4; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAB094e
Plasmid#79245PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4 (Y87A); PC araC
UseTagsExpressionBacterialMutationPromoterPBAD; PCAvailable sinceMay 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJT82_GalL_nCDA1Δ198-BE3
Plasmid#145046PurposeExpresses nCDA1Δ198-BE3 in yeast cellsDepositorInsertnCDA1Δ198-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-198AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB035h
Plasmid#79191PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-HA4
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB094a
Plasmid#79241PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPBAD rpoZ-HA4; PC araC
UseTagsExpressionBacterialMutationPromoterPBAD; PCAvailable sinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB082b
Plasmid#79234PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d3
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB082h
Plasmid#79240PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d23C
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
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pAB078d5
Plasmid#79204PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ppro1 434cI
UseTagsExpressionBacterialMutationPromoterPlacZ-opt (OR1); Ppro1Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB061i2
Plasmid#79229PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-HA4 (Y87A); PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPpro1; PlacIqAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB064d
Plasmid#79197PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 luxAB; Ppro1 434cI-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlac62; Ppro1Available sinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVSL10
Plasmid#201182PurposeBst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavableDepositorInsertBst polymerase large fragment (BF) F710Y/D598A
UseTagsHRV 3C protease cleavage site and His6ExpressionBacterialMutationF710Y D598APromoterT5-lacOAvailable sinceSept. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAB082a
Plasmid#79233PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d123
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Tyr-P(11)4
Plasmid#185784PurposeEncodes enzyme tyrosinase linked to P(11)4 self-assembling peptide via a GS-linker sequenceDepositorInsertTyr-P(11)4
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceJuly 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT43_GalL_BE-P(AP)3
Plasmid#145036PurposeExpresses BE-P(AP)3 in yeast cellsDepositorInsertBE-(AP)3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPSP6
Plasmid#48150PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT112_GalL_nCDA1-xBE3
Plasmid#141318PurposeExpressing base editor nCDA1-xBE3 in yeast cellsDepositorInsertnCDA1-xBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…PromoterGalLAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB035a
Plasmid#79190PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-SH2ABL1
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceDec. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1+t
Plasmid#48147PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT45_GalL_nCDA1-BE3
Plasmid#145038PurposeExpresses nCDA1-BE3 in yeast cellsDepositorInsertnCDA1-BE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB029f
Plasmid#79220PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-SH2ABL1; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAB031a
Plasmid#79221PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ HA4-Zif268; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceMarch 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2+t
Plasmid#48148PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pJT79_GalL_nCDA1Δ192-BE3
Plasmid#145045PurposeExpresses nCDA1Δ192-BE3 in yeast cellsDepositorInsertnCDA1Δ192-BE3
UseCRISPRTagsExpressionYeastMutationpmCDA1(1-192AA); spCas9(D10A)PromoterGalLAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAB043c
Plasmid#79223PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-p38-alpha; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCALNL-NaChBac
Plasmid#125580PurposeCre-dependent expression of NaChBac (FLAG-tagged) in mammalian cells.DepositorInsertNaChBac
UseCre/LoxTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB042b
Plasmid#79193PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-MBPoff7
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB042d
Plasmid#79194PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlac62 gIII, luxAB; Ptet rpoZ-p38_2_3
UseTagsExpressionBacterialMutationPromoterPlac62; PtetAvailable sinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAB043a
Plasmid#79222PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ lambda cI-MBP; PlacIq lacI
UseTagsExpressionBacterialMutationPromoterPlacZ; PlacIqAvailable sinceApril 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAB082f
Plasmid#79238PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d23A
UseTagsExpressionBacterialMutationPromoterPpro1Available sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBsVchDonor
Plasmid#203814PurposeDNA donor for transpositionDepositorInsertMini-Tn, aprE cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRD415
Plasmid#178197PurposeRok _Linker overexpressionDepositorInsertrok _linker
UseTagsExpressionBacterialMutationdeletion of residues 75-96PromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRD461
Plasmid#178198PurposeRok with a C-terminal His-tag overexpressionDepositorInsertrok 6xHis
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceNov. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pThuAsol
Plasmid#171675PurposepThuAsol expression in E. coliDepositorInsertSUMO-TEV-ThuAsol
UseTagsHis-tag and SUMO-tagExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJT140_GalL_cCDA1-VRERBE3
Plasmid#145103PurposeExpressing base editor cCDA1-VRERBE3 in yeast cellsDepositorInsertcCDA1-VRERBE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A/D1135V/G1218R/R1335E/T1337R)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT46_GalL_cCDA1-BE3
Plasmid#145039PurposeExpresses cCDA1-BE3 in yeast cellsDepositorInsertcCDA1-BE3
UseCRISPRTagsExpressionYeastMutationspCas9(D10A)PromoterGalLAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJT176_GalL_eA3A-BE3
Plasmid#145125PurposeExpressing base editor eA3A-BE3 in yeast cellsDepositorInsertA3A(N57G)-BE3
UseCRISPRTagsExpressionYeastMutationA3A(N57G); spCas9(D10A)PromoterGalLAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only