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We narrowed to 303 results for: eukaryotic cell

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Showing: 251 - 300 of 303 results

  1. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q25

    Plasmid
    #111743
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q25 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111743-plasmid-map-sequence-id-220479

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  2. pBacMam2-DiEx-LIC-C-flag_huntingtin_full-length_Q24

    Plasmid
    #111742
    Purpose
    Baculovirus transfer vector for expression of huntingtin protein in insect and mammalian cellscells as well as in mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Insert
    HTT (HTT Human)
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Q24 (polyQ repeat)
    Promoter
    CMV and P10
    Available Since
    Aug. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    111742-plasmid-map-sequence-id-220476

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  3. pYO1846

    Plasmid
    #235714
    Purpose
    Expression of mCherry-human Beclin 1 in mammalian cells
    Depositor
    Roger Williams
    Article
    Špokaitė et al eLife 15:RP110040
    Insert
    Beclin 1 (BECN1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only

  6. pYO1847

    Plasmid
    #235715
    Purpose
    Expression of FL human VPS15 S579D_H580D_T583E mutant -ZZ in mammalian cells
    Depositor
    Roger Williams
    Article
    Špokaitė et al eLife 15:RP110040
    Insert
    VPS15 (PIK3R4 Human)
    Tags
    3xTEV-ZZ
    Expression
    Mammalian
    Mutation
    S579D, H580D, T583E
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only

  7. pJL29

    Plasmid
    #174063
    Purpose
    Expresses for mCherry for S. cerevisiae. Contain 2 43-8 zinc-finger operators upstream of Cyc1 promoter.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    2x43-8 ZFO-1x97-4 ZFO pCyc mCherry tCyc
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    pCyc1
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174063-plasmid-map-sequence-id-341950

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  8. pYO1356

    Plasmid
    #235713
    Purpose
    Expression of FL human VPS34 R199E E200R E202R muntant-EGFP in mammalian cells
    Depositor
    Roger Williams
    Article
    Špokaitė et al eLife 15:RP110040
    Insert
    VPS34 (PIK3C3 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    R199E, E200R, E202R
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only

  9. pYO1344

    Plasmid
    #235711
    Purpose
    Expression of FL human VPS34 R199E E200R E202R mutant in mammalian cells
    Depositor
    Roger Williams
    Article
    Špokaitė et al eLife 15:RP110040
    Insert
    VPS34 (PIK3C3 Human)
    Expression
    Mammalian
    Mutation
    R199E, E200R, E202R
    Available Since
    April 25, 2025
    Availability
    Academic Institutions and Nonprofits only

  10. pJL50

    Plasmid
    #174068
    Purpose
    Expresses 97-3 zinc finger array fused to VP16 in S. cerevisiae. Parent plasmid for chromatin regulator library.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    pTEF1-3NLS-97-3ZF-VP16
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    pTEF1
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    174068-plasmid-map-sequence-id-341751

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  11. pJL30

    Plasmid
    #174064
    Purpose
    Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    pGal1
    Available Since
    Dec. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    174064-plasmid-map-sequence-id-341957

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  12. pJL15

    Plasmid
    #174069
    Purpose
    Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.
    Depositor
    Albert Keung
    Article
    Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(
    Insert
    pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1
    Tags
    FLAG
    Expression
    Yeast
    Promoter
    pGal1
    Available Since
    Sept. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174069-plasmid-map-sequence-id-342040

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  24. pLenti GW V5 Eco/Dam mse EMD

    Plasmid
    #182675
    Purpose
    Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.
    Depositor
    Bas van Steensel
    Article
    Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.
    Insert
    Emerin (Emd Mouse)
    Use
    Lentiviral
    Tags
    E. Coli Dam and V5
    Expression
    Mammalian
    Promoter
    HSP
    Available Since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    182675-plasmid-map-sequence-id-358621

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  25. pBacMam2-DiEx-LIC-N entry vector

    Plasmid
    #111751
    Purpose
    Intermediate vector containing HTT gene, except for polyQ region. Used to clone various polyQ lengths into HTT sequence with N-term FLAG tag. Baculovirus transfer vector for insect and mammalian cells
    Depositor
    Cheryl Arrowsmith
    Article
    Harding et al J Biol Chem. 2019 Apr 26;294(17):6986-70
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    FLAG
    Expression
    Mammalian
    Available Since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    111751-plasmid-map-sequence-id-247722

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  26. pSSA-DsRed/eGFP (pXK-442)

    Plasmid
    #208827
    Purpose
    The SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208827-plasmid-map-sequence-id-417251

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  27. pSSA-eGFP (pXK-221)

    Plasmid
    #208826
    Purpose
    The SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    208826-plasmid-map-sequence-id-417242

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  29. pLL3.7-hSt3Cas9 (pXK-1721)

    Plasmid
    #208828
    Purpose
    CRISPR/hSt3Cas9 expression vector for Animal gene editing, harboring the humanized St3Cas9 gene and the corresponding optimized SP1.gRNA. Key Construct: mU6- SP1.gRNA-CMV-hSt3Cas9.
    Depositor
    Kun Xu
    Article
    Xu et al Cell Mol Life Sci. 2015 Jan;72(2):383-99
    Type
    Empty backbone
    Use
    CRISPR; Stcas9
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    208828-plasmid-map-sequence-id-417250

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  37. pCBS-4559

    Plasmid
    #249331
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) in mammalian cells and gfp-dropout cassette for cloning sgRNAs
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249331-plasmid-map-sequence-id-497004

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  38. pCBS-5391

    Plasmid
    #249330
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and Non-targeting guide for mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249330-plasmid-map-sequence-id-497003

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  39. pCBS-5846

    Plasmid
    #249329
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A)and sg#3 targeting VEGFA gene in mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249329-plasmid-map-sequence-id-497002

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  40. pCBS-6756

    Plasmid
    #249328
    Purpose
    Expresses EPEC DarT2(G49D)-nSpCas9(D10A) and sg#2 targeting PTEN gene in mammalian cells
    Depositor
    Chase Beisel
    Article
    Gupta et al Nat Biotechnol. 2025 Sep 4. doi: 10.1038
    Insert
    EPEC DarT2(G49D)-nSpCas9(D10A)
    Tags
    3X Flag tag
    Expression
    Mammalian
    Mutation
    DarT2(G49D) and nSpCas9(D10A)
    Available Since
    Jan. 13, 2026
    Availability
    Academic Institutions and Nonprofits only
    249328-plasmid-map-sequence-id-497001

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Showing: 251 - 300 of 303 results