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pGAD-Atg17(∆266-312)

Plasmid

#121521

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Use

Tags

Expression

Yeast

Mutation

deleted amino acids 266-312

Promoter

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pHACK(Gal4)-DONR(T2A-LexAGAD)

Plasmid

#194769

Purpose

To insert T2A-LexAGAD into Gal4 coding sequence in Drosophila, converting Gal4 into a T2A-LexAGAD transgene.

Depositor

Chun Han

Article

Karuparti et al G3 (Bethesda). 2023 Mar 9;13(3):jkad003.

Insert

T2A-LexAGAD

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Gad65P-eGFP-3XNLS-WPRE-pA

Plasmid

#183804

Purpose

AAV vector to express nuclear localized eGFP from a Gad65 promoter

Depositor

Jonathan Ploski

Article

de Solis et al Brain Res. 2017 Oct 1;1672:148-162. doi:

Insert

eGFP-NLS

Use

AAV

Tags

NLS

Expression

Mutation

Promoter

Available since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-T2A-LexGAD-3xP3-RFP

Plasmid

#127558

Purpose

CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-LexGAD-SV40 and 3xP3-RFP visible eye marker.

Depositor

Norbert Perrimon

Article

Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

Insert

LexGAD

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV mGAD65(delE1) GFP WPRE SV40pA

Plasmid

#177316

Purpose

To produce the AAV vector that expresses GFP under the control of the mGAD65 promoter. The mGAD65 promoter has highly specificity to GABAergic interneurons.

Depositor

Hirokazu Hirai

Article

Hoshino et al Mol Brain. 2021 Feb 15;14(1):33. doi: 10

Insert

mGAD65(delE1) promoter, GABAergic interneurons specific promoter

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSBtet-Hygro-GADD34_Δ1-240/P2A/K3L

Plasmid

#196136

Purpose

SB vector for the mammalian expression of GADD34-K3L

Depositor

Jamie Cate

Article

Aleksashin et al bioRxiv. 2023 Feb 10:2023.02.09.527910.

Insert

GADD34 Δ1-240 - P2A - K3L (PPP1R15A Human, vaccinia virus)

Use

Tags

P2A

Expression

Mammalian

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pGADT7-ADH700-yeCherry-p150-yeGFP-DHFR

Plasmid

#24378

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::p150yeGFP-DHFR

Use

Tags

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78304

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pGADT7-ADH700-yeCherry-YAP1-yeGFP-DHFR

Plasmid

#24549

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::YAP1 yeGFP-DHFR (YAP1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78306

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 1

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADfluw-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

June 7, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70

Plasmid

#78307

Purpose

Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-nlsLexA::GADflluw

Use

Tags

Expression

Mutation

Promoter

Available since

July 8, 2016

Availability

Academic Institutions and Nonprofits only

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Anti-GAD67 [L127/8-rat IgG2a] - Chimeric

Plasmid

#227047

Purpose

Mammalian expression plasmid of anti-GAD67 (Homo sapiens) rat IgG2a R-mAb. Derived from hybridoma L127/8.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-GAD67 (Homo sapiens) recombinant mouse monoclonal antibody. (GAD1 Chimera: M. musculus (mouse)/R. norvegicus (rat))

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad65-WPRE-HGHpA

Plasmid

#184631

Purpose

Expresses Gad65, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad65 (Gad2 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

Plasmid

#184632

Purpose

Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

Depositor

Nicolas Tritsch

Article

Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

Insert

Gad67 (Gad1 Mouse)

Use

AAV

Tags

Expression

Mutation

Promoter

EF1a

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGADT7-ADH700-yeCherry-pTEF1 (S.C.)-yeGFP-DHFR

Plasmid

#24584

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::pTEF1 (S.C.)yeGFP-DHFR

Use

Tags

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Promoter

Available since

May 18, 2010

Availability

Academic Institutions and Nonprofits only

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pGADT7-ADH700-yeCherry-pTEF1(ashbya)-yeGFP-DHFR

Plasmid

#24585

Depositor

Thomas Wandless

Article

Edwards et al Yeast. 2010 Apr;27(4):229-36. doi: 10.10

Insert

pADH1 yeCherry::pTEF1 (Ashbya)yeGFP-DHFR

Use

Tags

Expression

Yeast

Mutation

1. Truncated version of ADH700 promoter (SbfI/Spe…

Promoter

Available since

May 3, 2010

Availability

Academic Institutions and Nonprofits only

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pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

Plasmid

#52890

Purpose

"CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

Depositor

Iswar Hariharan

Article

Bosch et al Development. 2015 Feb 1;142(3):597-606.

Inserts

LexGAD
Gal4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-3xT7

Plasmid

#171028

Purpose

Expresses 3xT7-tagged target in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Type

Empty backbone

Use

Retroviral

Tags

3x-T7

Expression

Mammalian

Mutation

Promoter

5' LTR

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS circfirefly_1677

Plasmid

#110118

Purpose

Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Firefly luciferase

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pJPS2951

Plasmid

#135585

Purpose

SNR6 has UU at position 105–106 deleted

Depositor

Jonathan Staley

Article

Toroney et al Genes Dev. 2019 Sep 26. pii: gad.328294.

Insert

snR6 (snR6 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

U6Δ2 has UU at position 105–106 deleted

Promoter

Native

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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