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Plasmid#124804PurposeHDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.DepositorInsertMurine IgG2b
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG2a-srt-his
Plasmid#124803PurposeHDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.DepositorInsertMurine IgG2a
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG1-srt-his
Plasmid#124802PurposeHDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.DepositorInsertMurine IgG1
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoter-Available sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mA-srt-his
Plasmid#124806PurposeHDR template to knock-in mouse IgA Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgA producing cell lines.DepositorInsertMurine IgA
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>mG3-srt-his
Plasmid#124805PurposeHDR template to knock-in mouse IgG3 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG3 producing cell lines.DepositorInsertMurine IgG3
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>m2a(silent)-srt-his
Plasmid#124807PurposeHDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.DepositorInsertMurine IgG2a (Fc silent)
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterial and MammalianMutationL234A/L235A/N297APromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHybr_r2a>Fab-srt-his
Plasmid#124810PurposeHDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.DepositorInsertrat IgG2a Hinge-G4S-Sortag-Histag
UseCRISPR; Hdr templateTagsHistag (HHHHHH) and Sortag (LPETGG)ExpressionBacterialMutationPromoterpUCAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p350_LTJ_180bpCD90.1Template
Plasmid#104988Purpose180bp HDR template for murine CD90.1DepositorInsert180bp genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p214_LTJ_1kbCD90.1Template
Plasmid#82667Purpose1kb HDR template for murine CD90.1DepositorInsert1kb genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p229_LTJ_2kbCD90.1Template
Plasmid#82668Purpose2kb HDR template for murine CD90.1DepositorInsert2kb genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p248_LTJ_2kbCD45.1Template
Plasmid#82662Purpose2kb HDR template for murine CD45.1DepositorInsert2kb genomic DNA sequence from murine CD45.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p213_LTJ_160bpCD90.1Template
Plasmid#82666Purpose160bp HDR template for murine CD90.1DepositorInsert160bp genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p230_LTJ_4kbCD90.1Template
Plasmid#82669Purpose4kb HDR template for murine CD90.1DepositorInsert4kb genomic DNA sequence from murine CD90.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p242_LTJ_1kbCD45.1Template
Plasmid#82661Purpose1kb HDR template for murine CD45.1DepositorInsert1kb genomic DNA sequence from murine CD45.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p243_LTJ_4kbCD45.1Template
Plasmid#82663Purpose4kb HDR template for murine CD45.1DepositorInsert4kb genomic DNA sequence from murine CD45.1
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p249_LTJ_1kbFoxp3wtTemplate
Plasmid#82664Purpose1kb HDR template for murine wildtype Foxp3DepositorInsert1kb genomic DNA sequence from murine wildtype Foxp3
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p250_LTJ_2kbFoxp3wtTemplate
Plasmid#82665Purpose2kb HDR template for murine wildtype Foxp3DepositorInsert2kb genomic DNA sequence from murine wildtype Foxp3
UseHdr template for gene editingTagsExpressionMutationPromoterAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
CMV-hRad51
Plasmid#125570PurposeExpresses the Rad51 protein in mammalian cells under CMV promoterDepositorInsertRad51 (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
hRad51(K133R)
Plasmid#125571PurposeExpresses the hRad51(K133R) protein in mammalian cells under CMV promoterDepositorInsertRad51 (RAD51 Human)
UseCRISPRTagsExpressionMammalianMutationK133RPromoterAvailable sinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD
Plasmid#216248PurposeHDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSDDepositorInsertHDR template for human RANGAP1 (RANGAP1 Human)
UseCRISPR and TALEN; Hdr templateTagsminiIAA7.3xFlag.P2A.BSDExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only