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Plasmid#207153PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAQ (GNAQ Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(124)EPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s
Plasmid#100020PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.DepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi2-91-NLuc
Plasmid#207149PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI2 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI2 (GNAI2 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)QPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gαi3-91-NLuc
Plasmid#207150PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI3 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNAI3 (GNAI3 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position L(91)KPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα13-125-NLuc
Plasmid#207155PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA13 tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorInsertGNA13 (GNA13 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position F(125)DPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)Gα12-133-NLuc
Plasmid#207156PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNA12 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2DepositorInsertGNA12 (GNA12 Human)
UseTagsExpressionMammalianMutationNLuc inserted at position A(133)FPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsS-246-Nluc
Plasmid#207157PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorInsertGNAS (GNAS Human)
UseTagsExpressionMammalianMutationNLuc inserted at position D(246)NPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)GαsL-260-NLuc
Plasmid#207158PurposePart of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2DepositorInsertGNAS (GNAS Human)
UseTagsExpressionMammalianMutationNLuc inserted at position D(260)NPromoterAvailable sinceNov. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
UseTagsNT* tag derived from spider silk proteinsExpressionBacterialMutationPromoterT7Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPT41
Plasmid#85524PurposeTitration sponge for TetRDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterPLtetAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPT145
Plasmid#85527PurposeTitration sponge for TetR, cI and lacIDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterPLtet, PLlac, PRAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPT148
Plasmid#85528PurposeTitration sponge for TetR and lacIDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterPLtet, PLlacAvailable sinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPT149
Plasmid#85529PurposeTitration sponge for TetR and cIDepositorTypeEmpty backboneUseTagsExpressionMutationPromoterPLtet, PRAvailable sinceOct. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLPT107
Plasmid#85525PurposeRepressilator with triple reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
mKate2
UseSynthetic BiologyTagsssrAExpressionBacterialMutationPromoterAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLPT119
Plasmid#85526PurposeRepressilator without degradation tags, with integrated reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
LT1_30_backbone_X2_integration
Plasmid#194403PurposeMulti Backbone with X2 homology arms for Saccharomyces cerevisiaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_31_backbone_HO_integration
Plasmid#194404PurposeMulti Backbone with HO homology arms for Saccharomyces cerevisiaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLPT20
Plasmid#85460PurposeRepressilator with integrated reporterDepositorInsertsmCfp
mVenus
tetR
lacI
cI
UseSynthetic BiologyTagsssrAExpressionBacterialMutationPromoterPLlac, PLtet, PR, and PRNAIAvailable sinceMay 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
LT1_78_TU2of2_HOhomology
Plasmid#194417PurposeTU2 backbone (for TU #2 for plasmid with 2 TUs) with HO homology arms for Saccharomyces cerevisiaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
LT1_79_TU2of3-6_HOhomology
Plasmid#194418PurposeTU2 backbone (for TU #2 for plasmid with 3-6 TUs) with HO homology arms for Saccharomyces cerevisiaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNAPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only