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pAMP-CY1_NF1 T2 (+minintr)

Plasmid

#119205

Purpose

transfer vector

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

NF1 type 2 mini-gene (NF1 Human)

Use

Transfer vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-Matr3 delZnF1

Plasmid

#32883

Depositor

Yossi Shiloh

Article

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 283-322

Promoter

CMV

Available since

Jan. 6, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/TO-RNF185-GFP

Plasmid

#113498

Purpose

Expression of human RNF185 with C-terminal GFP tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

RNF185 (RNF185 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMH-SFB-RNF169

Plasmid

#99392

Purpose

Mammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF169

Depositor

Michael Huen

Article

Chen et al J Biol Chem. 2012 Aug 10;287(33):27715-2

Insert

RNF169 (RNF169 Human)

Use

Tags

SFB (S protein-FLAG-Streptavidin binding peptide)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pLV-H1-SGIPZ_NF1 sh1miR

Plasmid

#119206

Purpose

knockdown of NF1

Depositor

Helen Morrison

Article

Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

Insert

sh1miR targeting NF1 (NF1 Human, Mouse, Rat)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-U6-RNF103-CHMP3

Plasmid

#140584

Purpose

Expresses a gRNA and mCherry in mammalian cells

Depositor

Feng Zhang

Article

Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

Insert

gRNA RNF103-CHMP3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-RNF138-delta 18-58

Plasmid

#78921

Purpose

Mammalian expression of RNF138 with delta 18-58 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 18-58

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pGEX4T2-Snf1-cat

Plasmid

#52683

Purpose

expression of the Snf1 kinase domain in bacteria

Depositor

Benjamin Turk

Article

Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

Insert

SNF1 (SNF1 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

encodes kinase domain only, aa 41-315

Promoter

tac

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pCAG-TetR-RNF169UBD

Plasmid

#119011

Purpose

Mammalian expression of a fusion protein of single chain Tet repressor with the human RNF169 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

TetR

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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GST-RNF126 C231A

Plasmid

#138644

Purpose

Bacterial expression of GST-RNF126 RING mutant (C231A) fusion protein

Depositor

Jane McGlade

Article

Smith et al J Cell Sci. 2013 Mar 15;126(Pt 6):1366-8

Insert

RNF126 (RNF126 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

C231A

Promoter

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-Myc-TRF2ZNF1-11

Plasmid

#87178

Purpose

Retroviral stable expression of chimeric protein TRF2ZNF1-11

Depositor

Eros Lazzerini Denchi

Article

Li et al Science. 2017 Jan 12. pii: aah6752. doi:

Insert

chimeric protein TRF2ZNF1-11

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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RNF168 C-terminal sgRNA

Plasmid

#207100

Purpose

pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GAGATGCACAAAGTAAGGCC

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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RNF149 shRNA-TRE

Plasmid

#225337

Purpose

shRNAmir backbone under tet-operator for RNA interference, with YFP

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF149 shRNA (Rnf149 Mouse)

Use

AAV and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

TRE (tetracycline-responsive element)

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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Human RNF130-3X FLAG

Plasmid

#225331

Purpose

Lentiviral expression of human RNF130 with 3x FLAG tag in mammalian cells

Depositor

Huda Zoghbi

Article

Kim et al Neuron. 2023 Mar 15;111(6):824-838.e7. d

Insert

RNF130 (RNF130 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169 C-terminal sgRNA

Plasmid

#207099

Purpose

pX330 based plasmid for expression of Cas9 and the ACACTTCATTAGGTGCTACT sgRNA to target the RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

ACACTTCATTAGGTGCTACT

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and U6

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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RNF169-Halo HRD

Plasmid

#207083

Purpose

Homologous recombination donor for insertion of a HaloTag at the C-terminus of the endogenous RNF169 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

HaloTag followed by BGH PolyA and PuroR cassette flanked by human RNF169 locus sequences

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Endogenous

Available since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNf1#2/Cre

Plasmid

#173608

Purpose

Expresses a Nf1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf1 (Nf1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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Lenti-sgNf1#1/Cre

Plasmid

#173607

Purpose

Expresses a Nf1-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Nf1 (Nf1 Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L1-dSpRY

Plasmid

#177681

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pYPQ-NF1-L2-dSpRY

Plasmid

#177682

Purpose

Gateway entry clone (attL1 & attR5) for dimeric FokI-dCas9-SpRY genome editing

Depositor

Yiping Qi

Article

Cheng et al Biotechnol J. 2022 Apr 4:e2100571. doi:

Insert

FokI-dCas9-SpRY

Use

CRISPR; Gateway compatible foki-dcas9-spry entry …

Tags

NLS

Expression

Plant

Mutation

Promoter

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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