Addgene

Showing: 101 - 117 of 117 results

Results per page:

Sema5a-Fc-His

Plasmid

#72161

Purpose

Expresses the extracellular region of the Sema5A protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Sema5a (Sema5a Mouse)

Use

Tags

Fc-His

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
AL05_pDonor.DMD

Plasmid

#100284

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AL62_pDonor.DMD.TS

Plasmid

#100287

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AC62_pDonor.DMD.TS.DR

Plasmid

#100288

Purpose

DMD intron 43-targeting plasmid

Depositor

Manuel Goncalves

Article

Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

Insert

EGFP expression unit (DMD Synthetic, Human)

Use

CRISPR and Synthetic Biology; Human targeting

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AA69_pU.CAG.Cas9-eSp(1.1)-D10A.rBGpA.2NLS

Plasmid

#199252

Purpose

Expression construct encoding the high specificity eSpCas9(1.1)-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity eSpCas9(1.1)-D10A nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A K1003A R1060A

Promoter

CAG promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

Plasmid

#199254

Purpose

Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

Insert

High specificity S. pyogenes Cas9-D10A-KARA nickase

Use

CRISPR

Tags

3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Mutation

D10A K848A R1060A

Promoter

CAG promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2935

Plasmid

#144411

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MAFK (MAFK Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

Enlarge
TFORF2950

Plasmid

#144426

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MAFB (MAFB Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.puro_shGLS

Plasmid

#110335

Purpose

shGLS (Target CAACTGGCCAAATTCAGTC), silence human GLS gene, puromycin selection

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS glutaminase (GLS Synthetic, Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

U6 (RNA PolIII)

Availability

Academic Institutions and Nonprofits only

Enlarge
pQC hGAB.wt V5-His IRES G418

Plasmid

#110333

Purpose

Mammalian retroviral expression of glutaminase 2 isoform GAB (wild type) with V5 and 6xHis tags

Depositor

Sandra Martha Gomes Dias

Article

Dias et al Oncogene. 2020 Jan;39(3):690-702. doi: 1

Insert

GLS2 Glutaminase 2 (GLS2 Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Mutation

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_ spike_del19

Plasmid

#155297

Purpose

Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

Depositor

Raffaele De Francesco

Article

Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

Insert

SARS-Cov2 spike_deleted (S SARS-Cov2)

Use

Generation of sars-cov-2-pseudotyped lentiviral p…

Tags

Expression

Mutation

Deletion in C-term 19 aa of spike

Promoter

CMV

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEF-hSEMA4C-Fc

Plasmid

#74449

Purpose

Expresses human Semaphorin-4C ectodomain fused to human Fc in mammalian cells

Depositor

Roland Friedel

Article

Le et al Oncotarget. 2015 Mar 30;6(9):7293-304.

Insert

human Semaphorin-4C ectodomain with human Fc tag (SEMA4C Human)

Use

Tags

human Fc tag

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Availability

Academic Institutions and Nonprofits only

Enlarge
pCRII-Sema4c-ISH-2kb

Plasmid

#68031

Purpose

Contains 2kb cDNA fragment for probe synthesis for mRNA in situ hybridization, species: mouse

Depositor

Roland Friedel

Article

Maier et al Mol Cell Neurosci. 2011 Feb;46(2):419-31

Insert

Semaphorin-4C (Sema4c Mouse)

Use

Mrna in situ hybridization probe

Tags

Expression

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SEMA5A-COMP5AP-AviTag-9xHis

Plasmid

#157572

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA5A (SEMA5A Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SEMA4C-COMP5AP-AviTag-9xHis

Plasmid

#157499

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA4C (SEMA4C Human)

Use

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Mutation

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SEMA4C-Fc(DAPA)-AviTag-6xHis

Plasmid

#156935

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA4C (SEMA4C Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

Enlarge
pD649-HAsp-SEMA5A-Fc(DAPA)-AviTag-6xHis

Plasmid

#157008

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA5A (SEMA5A Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Availability

Academic Institutions and Nonprofits only

Enlarge