Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 15,323 results for: pET

Showing: 2361 - 2400 of 15323 results

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pET28-hisSumo-KE-Des27.7_PROSS

Plasmid

#248012

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7 ModularAssembly&PROSS)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_PROSS

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-hisSumo-KE-Des27.7_ModularAssembly

Plasmid

#248011

Purpose

Expression of synthetic Kemp eliminase (Ablation of Des27.7. ModularAssembly)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7_ModularAssembly

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27

Plasmid

#247996

Purpose

Expression of synthetic Kemp eliminase (Des27)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.3

Plasmid

#247999

Purpose

Expression of synthetic Kemp eliminase (Des27.3)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.3

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.10

Plasmid

#248005

Purpose

Expression of synthetic Kemp eliminase (Des27.10)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.10

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.1

Plasmid

#247997

Purpose

Expression of synthetic Kemp eliminase (Des27.1)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.1

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.4

Plasmid

#248000

Purpose

Expression of synthetic Kemp eliminase (Des27.4)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.4

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.12

Plasmid

#248007

Purpose

Expression of synthetic Kemp eliminase (Des27.12)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.12

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61.1

Plasmid

#248010

Purpose

Expression of synthetic Kemp eliminase (Des61.1)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61.1

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.5

Plasmid

#248001

Purpose

Expression of synthetic Kemp eliminase (Des27.5)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.5

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des61

Plasmid

#248009

Purpose

Expression of synthetic Kemp eliminase (Des61)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des61

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.2

Plasmid

#247998

Purpose

Expression of synthetic Kemp eliminase (Des27.2)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.2

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.6

Plasmid

#248002

Purpose

Expression of synthetic Kemp eliminase (Des27.6)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.6

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.11

Plasmid

#248006

Purpose

Expression of synthetic Kemp eliminase (Des27.11)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.11

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.7

Plasmid

#248003

Purpose

Expression of synthetic Kemp eliminase (Des27.7)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.7

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.9

Plasmid

#248004

Purpose

Expression of synthetic Kemp eliminase (Des27.9)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.9

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28-hisSumo-KE-Des27.13

Plasmid

#248008

Purpose

Expression of synthetic Kemp eliminase (Des27.13)

Depositor

Sarel Fleishman

Article

Listov et al Nature. 2025 Jul;643(8074):1421-1427. do

Insert

KE-Des27.13

Tags

12xHis-bdSumo

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-VHH-mNeptune2.5

Plasmid

#243765

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mNeptune2.5. VHH-mNeptune2.5 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mNeptune2.5-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-hisMBP-rRNaseI

Plasmid

#216673

Purpose

Expresses hisMBP-tagged rat oxidation-resistant RNase inhibitor in E. coli hosts

Depositor

Po-Min Chiang

Article

Hsueh et al PLoS Biol. 2025 Dec 15;23(12):e3003537.

Insert

ribonuclease inhibitor (Rnh1 Rat)

Tags

his-tagged MBP

Expression

Bacterial

Promoter

T7

Available Since

Nov. 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
IQ-compete reporter (integrating)

Plasmid

#247176

Purpose

Plasmid for genomic integration of the IQ-compete reporter (GFP-TCS-Quencher-CL1) into the YPRCΔ15 locus of yeast cells.

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

GFP-TCS-Quencher-CL1

Expression

Yeast

Promoter

TEF1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
IQ-compete reporter (episomal)

Plasmid

#247217

Purpose

Single-copy plasmid expressing the IQ-compete reporter (GFP-TCS-Quencher-CL1).

Depositor

Johannes Herrmann

Article

Hoffman et al FEBS Lett. 2025 Oct 25. doi: 10.1002/187

Insert

GFP-TCS-Quencher-CL1

Expression

Yeast

Promoter

TEF1

Available Since

Nov. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET20b-His6-LONP1

Plasmid

#247937

Purpose

Expression of human LONP1 with methionine 115 as its first residue (mature mitochondrial-matrix localized form)

Depositor

Gabriel Lander

Article

Shin et al Nat Commun. 2021 May 28;12(1):3239. doi:

Insert

LONP1 (LONP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

encodes aa 115-959

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-VHH-mCardinal2

Plasmid

#243766

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to mCardinal2. VHH-mCardinal2 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and mCardinal2-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET24a-VHH-iRFP670

Plasmid

#243768

Purpose

Bacterial expression of a functionalized anti-GFP (VHH) nanobody fused to iRFP670. VHH-iRFP670 also contains a T7, HA, BAP and His6 epitope

Depositor

Dominik Buser, Martin Spiess

Article

Buser et al J Vis Exp. 2025 Oct 17;(224). doi: 10.37

Insert

anti-GFP nanobody VHH

Tags

T7-TEV site and iRFP670-HA-AviTag-6xHis

Expression

Bacterial

Promoter

T7

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-L-IGHJ4_2-His

Plasmid

#233335

Purpose

To express nanobody IGHJ4_2 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_2-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-L-IGHJ4_3-His

Plasmid

#233336

Purpose

To express nanobody IGHJ4_3 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_3-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-L-IGHJ4_4-His

Plasmid

#233337

Purpose

To express nanobody IGHJ4_4 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ4_4-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-L-IGHJ5-His

Plasmid

#233338

Purpose

To express nanobody IGHJ5 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-IGHJ5-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_His-TEV-SpyCatcher003-C

Plasmid

#233339

Purpose

To express SpyCatcher

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

His-TEV-SpyCatcher003-C

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_C6-Cys-His

Plasmid

#233323

Purpose

To express nanobody C6 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

C6-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_VHH16-Cys-His

Plasmid

#233324

Purpose

To express nanobody VHH16 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH16-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_VHH21-Cys-His

Plasmid

#233325

Purpose

To express nanobody VHH21 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

VHH21-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_IGHJ4_1-Cys-His

Plasmid

#233326

Purpose

To express nanobody IGHJ4_1 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_1-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_IGHJ4_2-Cys-His

Plasmid

#233327

Purpose

To express nanobody IGHJ4_2 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_2-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_IGHJ4_3-Cys-His

Plasmid

#233328

Purpose

To express nanobody IGHJ4_3 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_3-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_IGHJ4_4-Cys-His

Plasmid

#233329

Purpose

To express nanobody IGHJ4_4 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ4_4-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_IGHJ5-Cys-His

Plasmid

#233330

Purpose

To express nanobody IGHJ5 with C terminal cysteine

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

IGHJ5-Cys-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-Linker-C6-His

Plasmid

#233331

Purpose

To express nanobody C6 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-Linker-C6-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-L-VHH16-His

Plasmid

#233332

Purpose

To express nanobody VHH16 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH16-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_SpyTag003-L-VHH21-His

Plasmid

#233333

Purpose

To express nanobody VHH21 with N terminal SpyTag

Depositor

Magnus Rueping

Article

Gutu et al Curr Protoc. 2025 Oct;5(10):e70213. doi:

Insert

SpyTag003-L-VHH21-His

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 2361 - 2400 of 15323 results

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