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We narrowed to 8,244 results for: 11

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Showing: 1181 - 1200 of 8244 results

  2. pCas9_sgRNA_0

    Plasmid
    #70763
    Purpose
    expresses Ustilago maydis codon-optimized Cas9, contains U. maydis U6 promoter, is self-replicating
    Depositor
    Regine Kahmann
    Publication
    Schuster et al Fungal Genet Biol. 2015 Sep 11. pii: S10
    Inserts
    U6 promoter
    cas9
    tnos terminator
    Use
    Self-replicating in ustilago maydis, conferring c…
    Tags
    N-terminal NLS, C-terminal HA-tag +NLS
    Mutation
    Streptococcus pyogenes gene codon-optimized for U…
    Promoter
    U6 from Ustilago maydis and synthetic otef promot…
    Available since
    Nov. 12, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    70763-plasmid-map-sequence-id-240922

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  3. pEGFP-MyosinVa-Ctail

    Plasmid
    #110169
    Purpose
    Dominant negative effect by competing with MyosinVa C-terminal tail interactions
    Depositor
    Jose Esteban
    Publication
    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Myosin Va (Myo5a Mouse)
    Tags
    EGFP and Myc
    Expression
    Mammalian
    Mutation
    amino acids 1444-1853
    Promoter
    CMV
    Available since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    110169-plasmid-map-sequence-id-210484

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  5. pSG4K5

    Plasmid
    #74492
    Purpose
    For cloning and constitutive expression of sgRNAs in E.coli. New sgRNAs are cloned into SapI sites and multiplex sgRNAs are cloned into BsaI sites. Kanamycin resistant with pSC101 origin of rep.
    Depositor
    Xiao Wang
    Publication
    Standage-Beier et al ACS Synth Biol. 2015 Nov 20;4(11):1217-2
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23119
    Available since
    May 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    74492-plasmid-map-sequence-id-140493

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  7. p55-H2B-Dendra2

    Plasmid
    #80609
    Purpose
    Encodes H2B-Dendra2-fusion protein under the CAG promoter. Linearize using Asc1 and Not1 for mouse embryo injection.
    Depositor
    Periklis Pantazis
    Publication
    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    H2B-Dendra2 (H2BC21 Human, Synthetic)
    Tags
    Dendra2
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Feb. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    80609-plasmid-map-sequence-id-159250

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  8. msfGFP-Cx43

    Plasmid
    #69024
    Purpose
    Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.
    Depositor
    David Spray
    Publication
    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cx43 (Gja1 Rat)
    Tags
    sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    69024-plasmid-map-sequence-id-127982

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  11. SpCas9-NG

    Plasmid
    #138566
    Purpose
    Expresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    SpCas9-NG
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    L1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    138566-plasmid-map-sequence-id-274798

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  13. CYP11A1 (3N9Y)

    Plasmid
    #28119
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CYP11A1:HPC09N-C05:C214491 (CYP11A1 Human)
    Expression
    Bacterial
    Available since
    Feb. 28, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pBEAST_J23101-bphC

    Plasmid
    #128137
    Purpose
    The cell-free adapted backbone, pBEAST, expressing bphC (the enzyme converting biphenyl-2,3-diol to 2-hydroxy-6-oxo-6-phenylhexa-2,4-) under control of the constitutive promoter J23101, and RBS B0032
    Depositor
    Jean-Loup Faulon
    Publication
    Pandi et al Nat Commun. 2019 Aug 28;10(1):3880. doi:
    Insert
    bphC
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    J23101
    Available since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128137-plasmid-map-sequence-id-252649

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  16. pcDNA3.1.Gαq-LgB97

    Plasmid
    #134360
    Purpose
    Nanoluc complementation assay. Expression of Gαq protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gαq. Addition of the HA epitope at N terminus of Gαq.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαq-LgB97 (GNAQ Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134360-plasmid-map-sequence-id-306401

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  20. Tet-pLKO-FOXM1-shRNA-#1

    Plasmid
    #89498
    Purpose
    Lentiviral vector with dox inducible expression of shRNA targeting human FOXM1.
    Depositor
    Adam Karpf
    Insert
    FOXM1 (FOXM1 Human)
    Use
    Lentiviral and RNAi; Doxycycline inducible
    Expression
    Mammalian
    Promoter
    H1/TO
    Available since
    June 15, 2017
    Availability
    Academic Institutions and Nonprofits only
    89498-plasmid-map-sequence-id-181255

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Showing: 1181 - 1200 of 8244 results