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pME_Cp_0_7-8_005_Kan/ColEI_mScarlet-I

Plasmid

#235971

Purpose

Kanamycin/ColEI backbone with mScarlet-I placeholder for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Kan/ColEI

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_6_026_3C6SF

Plasmid

#235954

Purpose

3' Connector 6 Short Forward in the M2 position used to dictate position for lvl2 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

3C6SF

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_030_rpoB2_3'UTR_Cr

Plasmid

#235921

Purpose

rpoB2 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rpoB2 3'UTR

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_005_psbH_3'Hom_Cr

Plasmid

#235939

Purpose

3' Homology psbH in the M2 position used for genome integration at the psbH locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbH 3'Homology

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_013_psaB_5'UTR_Cr

Plasmid

#235840

Purpose

psaB 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaB 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_020_psaC_3'UTR_Cr

Plasmid

#235917

Purpose

psaC 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaC 3'UTR

Use

Synthetic Biology

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_015_psbC_5'UTR_Cr

Plasmid

#235842

Purpose

psbC 5'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbC 5'UTR

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_G_0_3_001_5'UTR_placeholder

Plasmid

#235830

Purpose

Encodes a sfGFP cassette in the 5'UTR position used for placeholder cloning

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

5'UTR placeholder

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_3_032_rbcL_5'UTR_full_Nt

Plasmid

#235851

Purpose

rbcL 5'UTR full Nicotiana tabacum

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

rbcL full 5'UTR Nt

Use

Synthetic Biology

Mutation

none

Available Since

Oct. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN-DEST (JDW 471)

Plasmid

#242573

Purpose

Gateway destination vector with a CAGGS promoter in the backbone.

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Type

Empty backbone

Use

Gateway subcloning

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2

Plasmid

#244111

Purpose

Cytosolic expression of green glucose sensor

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.HaloTag

Plasmid

#244105

Purpose

Bacterial expression of green glucose sensor with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAGFLEX.(cyto).iGlucoSnFR2

Plasmid

#244078

Purpose

Cytosolic expression of green glucose sensor with non-responsive HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2

Use

AAV

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pNoRBP.iGlucoSnFR2.H348H.HaloTag

Plasmid

#244108

Purpose

Bacterial expression of green glucose sensor (high affinity) with inert HaloTag

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.H348H.HaloTag

Tags

HaloTag

Expression

Bacterial

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_046_petD_3'UTR_Nt

Plasmid

#235931

Purpose

petD 3'UTR Nicotiana tabacum

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

petD 3'UTR

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_7-8_003_Amp/ColEI_mScarlet-I

Plasmid

#235969

Purpose

Ampicillin/ColEI backbone with mScarlet-I placeholder for lvl1 assembly

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

Amp/ColEI

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_6_020_psbA_5'Hom_rev_Cr

Plasmid

#235945

Purpose

5'Homology psbA reverse in the M2 position used for genome integration at the psbA locus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psbA 5'Homology reverse

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_048_psaC_3'UTR_Nt

Plasmid

#235933

Purpose

psaC 3'UTR Nicotiana tabacum

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

psaC 3'UTR

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_047_TMV_3'UTR_Tv

Plasmid

#235932

Purpose

TMV 3'UTR Tobacco Mosaic Virus

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

TMV 3'UTR

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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pME_Cp_0_5_015_petA_3'UTR_Cr

Plasmid

#235914

Purpose

petA 3'UTR Chlamydomonas reinhardtii

Depositor

Tobias Erb

Article

Inckemann et al Nat Plants. 2025 Nov;11(11):2332-2349. d

Insert

petA 3'UTR

Use

Synthetic Biology

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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