Addgene

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pXJ40 GADD34 (Delta Pest1)

Plasmid

#78096

Purpose

mammalian expression of GADD34 Delta Pest1

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

deletion of PEST1

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pEBB-3XMyc-TRAF2 S11A

Plasmid

#44105

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Tags

3xMyc

Expression

Mammalian

Mutation

Serine 11 to Alanine

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
LUC56 (Luc/UGA/SP)

Plasmid

#112147

Purpose

Mammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activity

Depositor

Paul Copeland

Article

Shetty et al J Biol Chem. 2014 Sep 5;289(36):25317-26

Insert

Luciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type SELENOP 3'UTR (Selenop Rat, Photinus pyralis)

Use

Tags

Expression

Mammalian

Mutation

Cysteine 258mutated to selenocysteine (U)

Promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLdSaCN

Plasmid

#123261

Purpose

Expresses Staphylococcus aureus Cas9 (SaCas9) and its gRNA in Leishmania

Depositor

Greg Matlashewski

Article

Zhang et al mSphere. 2019 Aug 21;4(4). pii: 4/4/e004

Insert

gRNA and SaCas9

Use

CRISPR; Leishmania

Tags

Expression

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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PB-HD118

Plasmid

#172655

Purpose

Expressing paired pegRNAs from human U6 and H1 promoters to make 118-bp deletion on HPRT1 gene

Depositor

Jay Shendure

Article

Choi et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

pegRNA-HD118A/pegRNA-HD118B

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pXJ40 GADD34 (514-674aa)

Plasmid

#75480

Purpose

mammalian expression of GADD34 514-674 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 514-674

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
FusionRed-BaxAlpha-C-15

Plasmid

#56101

Purpose

Localization: , Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

BaxAlpha

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
CBP-Bromo-SP65

Plasmid

#21089

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

Bromo domain aa 1107-1172

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pKT-SCFP3A-HIS

Plasmid

#64686

Purpose

pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

SCFP3A

Use

Tags

Expression

Yeast

Mutation

Nucleotide sequence has been yeast-codon optimize…

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

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pXJ40 GADD34 (1-513aa)

Plasmid

#75479

Purpose

mammalian expression of GADD34 1-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 1-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pXJ40 GADD34 (214-513aa)

Plasmid

#78095

Purpose

mammalian expression of GADD34 214-513 aa

Depositor

Shirish Shenolikar

Article

Choy et al Cell Rep. 2015 Jun 30;11(12):1885-91. do

Insert

GADD34 (PPP1R15A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

expresses aa 214-513

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only
pKT-mCitrineV163A-HIS

Plasmid

#64685

Purpose

pKT vector encoding yeast-codon optimized yellow fluorescent protein mCitrine V163A. The V163A mutation confers fast protein maturation.

Depositor

Erin O'Shea

Article

Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

Insert

mCitrine V163A

Use

Tags

Expression

Yeast

Mutation

Valine 163 changed to alanine

Promoter

Available since

April 29, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
CBP-HAT-SP65

Plasmid

#21088

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Expression

Mutation

HAT domain aa 1172-1621

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
HOXA9 (murine) HIS-tag pET

Plasmid

#8492

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HoxA9 (Hoxa9 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

BamHI/BalI fusion into amino acid #2 of HOXA9; ma…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
XE93 mFZ3-CS2+

Plasmid

#16804

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

mFZ3-CS2+ (Fzd3 Mouse)

Use

Xenopus expression

Tags

Expression

Mutation

Promoter

Available since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
pLX-V5-TRAF2 S11A-puro

Plasmid

#44112

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S11A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Serine 11 to Alanine

Promoter

Available since

July 22, 2013

Availability

Academic Institutions and Nonprofits only
CBP-CH3-SP65

Plasmid

#21092

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Expression

Mutation

CH3 domain aa 1625-1877

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pLX-V5-TRAF2 S102A-puro

Plasmid

#44113

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 S102A (TRAF2 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Serine 102 to Alanine

Promoter

Available since

April 2, 2013

Availability

Academic Institutions and Nonprofits only
FusionRed-CytERM-N-17

Plasmid

#56110

Purpose

Localization: Endoplasmic Reticulum, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

CytERM

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits
XE107 XPKC alpha-GFP-CS2+

Plasmid

#16743

Depositor

Randall Moon

Article

Sheldahl et al Curr Biol. 1999 Jul 1;9(13):695-8. doi:

Insert

PKC-alpha (prkca Frog)

Use

Xenopus expression

Tags

GFP

Expression

Mutation

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only