We narrowed to 49,092 results for: prot

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pTF

Plasmid

#162784

Purpose

Protein expression in CHO cells - C-terminal FLAG and 10xhis tags are coded

Depositor

Ivan Vorobiev

Article

Kolesov et al Diagnostics (Basel). 2022 Feb 3;12(2). p

Type

Empty backbone

Tags

10xHis and FLAG

Expression

Mammalian

Promoter

CHO Elongation factor 1A

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8

Plasmid

#175796

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_SS

Plasmid

#175798

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing disulfide 'staples'

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_SS

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub8_SH2sub6

Plasmid

#175799

Purpose

de novo thick circular tandem repeat protein trimer formed from 8-repeat monomers containing 2 copies of human Nck2 SH2

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub8_SH2sub6

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP9sub3

Plasmid

#175794

Purpose

de novo thick circular tandem repeat protein trimer formed from 3-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP9sub3

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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tcTRP24sub6

Plasmid

#175795

Purpose

de novo thick circular tandem repeat protein tetramer formed from 6-repeat monomers

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

tcTRP24sub6

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-10xQb oligo-library based

Plasmid

#158201

Purpose

Cassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.

Depositor

Roee Amit

Article

Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:

Insert

10 binding sites of QB based on oligo library experiment

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 R14A/K310A

Plasmid

#162655

Purpose

Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids R14 and K310 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K435A

Plasmid

#162651

Purpose

Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acid K435 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-ORF68 K450A/R451A

Plasmid

#162653

Purpose

Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

ORF68

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Amino acids K450 and R451 mutated to alanine

Promoter

CMV

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

Plasmid

#163925

Purpose

Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor

Use

Cre/Lox

Tags

tagBFP

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pET_4E2H.210

Plasmid

#155212

Purpose

Expresses 4E2H.210 de novo protein in E.coli

Depositor

Bruno Correia

Article

Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

Insert

4E2H.210

Tags

6x His Tag

Expression

Bacterial

Available Since

Aug. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-3G124-HilA

Plasmid

#153324

Purpose

Shows that expression of the protein of interest from Salmonella is disrupted when SptP signal is removed from the pCASP-HilA vector

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

3G124 DARPin but no expression

Use

Salmonella expression

Available Since

July 16, 2020

Availability

Academic Institutions and Nonprofits only

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D32_Ld_354780

Plasmid

#135848

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Hsp70 protein, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

July 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEU3-NII-GLICNot-C-BIOT

Plasmid

#140186

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, GST-tag and biotinylation site, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

Biotinylation and GST

Promoter

T7

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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D72_Ld_231020

Plasmid

#135885

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, unknown function

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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D30_Ld_260440

Plasmid

#135846

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D26_Ld_200260

Plasmid

#135842

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

transmembrane protein, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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D28_Ld_241170

Plasmid

#135844

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
D29_Ld_251870

Plasmid

#135845

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

hypothetical protein, conserved

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge