pCMV-T7-ABE8e-nSpCas9-NG-P2A-EGFP (KAC999)
Plasmid
#185914
Purpose
pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
Depositor
Insert
human codon optimized ABE8e-nSpCas9-NG-P2A-EGFP
Use
In vitro transcription; t7 promoter
Tags
BPNLS and BPNLS-3xFLAG-P2A-EGFP
Expression
Mammalian
Mutation
nSpCas9-NG=D10A/L1111R/D1135V/G1218R/E1219F/A1322…
Promoter
CMV and T7
Available Since
July 20, 2022
Availability
Academic Institutions and Nonprofits only
