165,528 results

1. pHR-FUSN-mCh-Cry2WT

   Plasmid

   #101223

   Purpose

   FUS(1-214) fused to mCh-Cry2WT

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

   Insert

   FUS(aa1-214) (FUS Human)
   

   Use

   Lentiviral

   Tags

   mCherry-Cry2

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1

   Plasmid

   #138417

   Purpose

   CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1

   Depositor

   Jian Xu

   Article

   Liu et al Genome Biol. 2020 Mar 5;21(1):59. doi: 1

   Inserts

   BirA
   dCas9
   

   Use

   Lentiviral

   Tags

   FLAG, Avi-tag and V5, His

   Promoter

   EF1a

   Available Since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDest-527

   Plasmid

   #11518

   Purpose

   Gateway destination vector for bacterial expression; His tag

   Depositor

   Dominic Esposito

   Type

   Empty backbone

   Use

   Gateway destination vector

   Tags

   6xHis

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDualEX-SMN2

   Plasmid

   #214431

   Purpose

   Dual-color reporter express eGFP constitutively; tdTomato expression is contingent on the exclusion of the frame-shifting cassette exon 7

   Depositor

   Chaolin Zhang

   Article

   Recinos et al Nat Commun. 2024 May 7;15(1):3839. doi:

   Insert

   SMN2 EXON 7 (SMN2 Human)
   

   Expression

   Mammalian

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SpyTag003-MBP

   Plasmid

   #133450

   Purpose

   Expresses SpyTag003-MBP (maltose binding protein) in bacterial cytoplasm

   Depositor

   Mark Howarth

   Article

   Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

   Insert

   SpyTag003-MBP
   

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to N-terminus of MBP via a GSGE…

   Promoter

   T7

   Available Since

   Dec. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

   Plasmid

   #103871

   Purpose

   dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRL623

   Plasmid

   #58494

   Purpose

   helper plasmid for bacterial conjugal DNA transfer

   Depositor

   Peter Wolk

   Article

   Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

   Insert

   encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
   

   Available Since

   Oct. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pN1-TOMM20-mScarletI

   Plasmid

   #129346

   Purpose

   Mitochondrial outer membrane localization

   Depositor

   Yasushi Okada

   Insert

   mScarlet(i) (Addgene 85044)
   

   Expression

   Mammalian

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBV-Luc

   Plasmid

   #16539

   Purpose

   luciferase reporter plasmid with very low basal activity

   Depositor

   Bert Vogelstein

   Article

   He et al Cell. 1999 Oct 29. 99(3):335-45.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Luciferase

   Expression

   Mammalian

   Available Since

   July 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pINDUCER20_3xFlag

    Plasmid

    #190028

    Purpose

    Doxycycline-inducible lentiviral vector for expression of ORF with C-terminal 3xFlag

    Depositor

    Andrew Tutt

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge