Purpose
BirA* expressed as a protein fusion with MS2 coat protein (MCP) dimer and EGFP; to visualize any MS2 tagged mRNAs and to identify the RNA proximal proteome by their biotinylation.
Insert
NLS-HA-2xMCP-EGFP-cMycBirA*
Use
Lentiviral
Tags
NLS, HA, cMyc (N-terminal of BirA*)
Expression
Mammalian
Mutation
BirA* = BIrA >R118G
Promoter
human ubiquitin C (UBC)
Available since
Aug. 30, 2019
Availability
Academic Institutions and Nonprofits only