Skip to main content
Addgene
Showing: 3001 - 3050 of 18430 results

  11. pNR4A1-donor

    Plasmid
    #112295
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP
    Depositor
    Kevin White
    Insert
    NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:12:52058840:C>T, hg38:12:52058930:A>C,…
    Promoter
    Available since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112295-plasmid-map-sequence-id-228416

    Enlarge

  12. mscv2.2-NAIP1

    Plasmid
    #60200
    Purpose
    Mouse NAIP1 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NAIP1
    Depositor
    Russell Vance
    Article
    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi
    Insert
    NAIP1
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    LTR
    Available since
    Feb. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
    60200-plasmid-map-sequence-id-96377

    Enlarge

  15. mscv2.2-6mycPrgJ

    Plasmid
    #60204
    Purpose
    6myc-tagged PrgJ (SPI-1 rod protein) from Salmonella typhimurium, under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of PrgJ
    Depositor
    Russell Vance
    Article
    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi
    Insert
    PrgJ
    Use
    Retroviral
    Tags
    6xMYC
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    60204-plasmid-map-sequence-id-96381

    Enlarge

  16. pJ151-HDR

    Plasmid
    #113631
    Purpose
    Contains two recombination homology arms and loxP-flanked cassette encoding puromycin resistance gene, Venus fluorescence marker, thymidine kinase suicide gene, for scarless chromosomal engineering.
    Depositor
    Alex Kentsis
    Inserts
    Left homology arm
    Puromycin resistance gene
    Venus fluorescence gene
    Thymidine kinase
    Right homology arm
    Use
    Cre/Lox; Homology recombination selection vector
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    EF1a promoter, EF1a promoter (T2A cleaving sequen…
    Available since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    113631-plasmid-map-sequence-id-222216

    Enlarge

  17. pUC-T7-J1~398-Luc

    Plasmid
    #208639
    Purpose
    for in vitro transcription template preparation, insert T7 promoter, JFH1(AB047639)_1~398 and F-luc from pGL3-Basic, into pUC19
    Depositor
    Quanfu Ma
    Insert
    Hepatitis C virus (HCV) strain JFH1(AB047639) nt 1~398
    Use
    Luciferase; In vitro transcription template
    Tags
    Firefly Luciferase (F-Luc and T7 promoter
    Expression
    Mutation
    nt 1~398, in frame with donstream F-Luc
    Promoter
    T7
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    208639-plasmid-map-sequence-id-418341

    Enlarge

  21. mscv2.2-6mycFlaA

    Plasmid
    #60203
    Purpose
    6xMYC-tagged full-length FlaA from Legionella pneumophila, expressed under the constitutive, moderate-level retroviral LTR promoter for mammalian cell expression; IRES-GFP reporter downstream of FlaA
    Depositor
    Russell Vance
    Article
    Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi
    Insert
    FlaA
    Use
    Retroviral
    Tags
    6xMYC
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    60203-plasmid-map-sequence-id-96380

    Enlarge

  23. pMAZ-donor

    Plasmid
    #112359
    Purpose
    CRISPR donor plasmid to tag human transcription factor MAZ with GFP
    Depositor
    Kevin White
    Insert
    MAZ homology arms flanking EGFP-IRES-Neo cassette (MAZ Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:16:29809765:G>A
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112359-plasmid-map-sequence-id-229679

    Enlarge

  24. pJUNB-donor

    Plasmid
    #112313
    Purpose
    CRISPR donor plasmid to tag human transcription factor JUNB with GFP
    Depositor
    Kevin White
    Insert
    JUNB homology arms flanking EGFP-IRES-Neo cassette (JUNB Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:19:12792992:C>G, hg38:19:12793018:T>G,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112313-plasmid-map-sequence-id-228468

    Enlarge

  25. pHEY1-donor

    Plasmid
    #112297
    Purpose
    CRISPR donor plasmid to tag human transcription factor HEY1 with GFP
    Depositor
    Kevin White
    Insert
    HEY1 homology arms flanking EGFP-IRES-Neo cassette (HEY1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:79765311:G>A
    Promoter
    Available since
    Oct. 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    112297-plasmid-map-sequence-id-225585

    Enlarge

  26. pDLX1-donor

    Plasmid
    #112343
    Purpose
    CRISPR donor plasmid to tag human transcription factor DLX1 with GFP
    Depositor
    Kevin White
    Insert
    DLX1 homology arms flanking EGFP-IRES-Neo cassette (DLX1 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:2:172087403:G>C,hg38:2:172087433:G>A,h…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112343-plasmid-map-sequence-id-229657

    Enlarge

  29. pE2F5-donor

    Plasmid
    #112351
    Purpose
    CRISPR donor plasmid to tag human transcription factor E2F5 with GFP
    Depositor
    Kevin White
    Insert
    E2F5 homology arms flanking EGFP-IRES-Neo cassette (E2F5 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:8:85213859:T>C, hg38:8:85213860:T>G, h…
    Promoter
    Available since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112351-plasmid-map-sequence-id-229665

    Enlarge

  30. pZNF354B-donor

    Plasmid
    #112309
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF354B with GFP
    Depositor
    Kevin White
    Insert
    ZNF354B homology arms flanking EGFP-IRES-Neo cassette (ZNF354B Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    hg38:5:178884427:G>A, hg38:5:178883445:C>T,…
    Promoter
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    112309-plasmid-map-sequence-id-228461

    Enlarge

  31. Split 'n coexpress CUL3-RBX1

    Plasmid
    #52293
    Purpose
    bacterial co-expression of N-terminal Cul3, C-terminal Cul3, and Rbx1
    Depositor
    Brenda Schulman
    Article
    Zhuang et al Mol Cell. 2009 Oct 9;36(1):39-50. doi: 1
    Inserts
    Cul3 N-terminal domain (CUL3 Human)
    Cul3 C-terminal domain (CUL3 Human)
    RBX1 (Rbx1 Mouse)
    Use
    Tags
    GST, IRES, and TEV
    Expression
    Bacterial
    Mutation
    contains aa 1-309, contains aa 310-768, and conta…
    Promoter
    IRES and tac
    Available since
    March 21, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 3001 - 3050 of 18430 results