We narrowed to 4,045 results for: golden gate
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Plasmid#217505PurposepSEVAb (SEVA 3.1), Type IIS compatible plasmid with sfGFP under the BBa_J23119 promoter, pSEVAb89 backbone, pBR322/ROP oriDepositorInsertsuperfolder green fluorescent protein (sfGFP)
UseSynthetic BiologyMutationS30R, Y39N, F64L, S65T, Q80R, F99S, N105T, Y145F,…Available SinceJuly 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAD183_gCAD_pET
Plasmid#239119PurposeGorilla ACOD1 in E.coli expression vectorDepositorInsertACOD1
TagsStrepTag-II and TEV siteExpressionBacterialPromoterT7Available SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3668_pGEEC578_pShip-EFS-rtTA-P2A-iRFP720-bGH
Plasmid#239741PurposePlasmid expressing a doxycycline-inducible transcriptional activatorDepositorInsertrtTA-P2A-iRFP720
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2499_pGEEC531_pShip-CMV-mRuby2-WPRE
Plasmid#239727PurposePlasmid expressing mRuby2 with the CMV promoter and WPRE 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2906_pGEEC546_pShip-EFS-tagBFP-bGH
Plasmid#239734PurposePlasmid expressing tagBFP with the EFS promoterDepositorInserttagBFP
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2907_pGEEC547_pShip-hPGK-tagBFP-bGH
Plasmid#239735PurposePlasmid expressing tagBFP with the hPGK promoterDepositorInserttagBFP
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2897_pGEEC548_pShip-UbC-tagBFP-bGH
Plasmid#239736PurposePlasmid expressing tagBFP with the UbC promoterDepositorInserttagBFP
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2899_pGEEC550_pShip-CMV-tagBFP-bGH
Plasmid#239737PurposePlasmid expressing tagBFP with the CMV promoterDepositorInserttagBFP
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2900_pGEEC551_pShip-CAG-tagBFP-bGH
Plasmid#239738PurposePlasmid expressing tagBFP with the CAG promoterDepositorInserttagBFP
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2023_pGEEC509_pShip-UbC-mRuby2-WPRE
Plasmid#239715PurposePlasmid expressing mRuby2 with the UbC promoter and WPRE 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2116_pGEEC510_pShip-EF1a-mRuby2-bGH
Plasmid#239716PurposePlasmid expressing mRuby2 with the EF1a promoter and bGH 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2117_pGEEC511_pShip-EF1a-mRuby2-SV40
Plasmid#239717PurposePlasmid expressing mRuby2 with the EF1a promoter and SV40 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2118_pGEEC512_pShip-EF1a-mRuby2-WPRE
Plasmid#239718PurposePlasmid expressing mRuby2 with the EF1a promoter and WPRE 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2075_pGEEC516_pShip-CAG-mRuby2-bGH
Plasmid#239719PurposePlasmid expressing mRuby2 with the CAG promoter and bGH 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2076_pGEEC517_pShip-CAG-mRuby2-SV40
Plasmid#239720PurposePlasmid expressing mRuby2 with the CAG promoter and SV40 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2077_pGEEC518_pShip-CAG-mRuby2-WPRE
Plasmid#239721PurposePlasmid expressing mRuby2 with the CAG promoter and WPRE 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2309_pGEEC524_pShip-EFS-ZF10-NS3-p65-bGH
Plasmid#239722PurposePlasmid expressing a grazoprevir-inducible transcriptional activatorDepositorInsertZF10-NS3-p65
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2215_pGEEC525_pShip-ZF43x6-C-mRuby2-bGH
Plasmid#239723PurposePlasmid expressing mRuby2 from a COMET promoter, Induced with pKG3666DepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2410_pGEEC526_pShip-TRE3G-mRuby2-bGH
Plasmid#239724PurposePlasmid expressing mRuby2 from the TRE3G promoter, Induced with pKG3668DepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2497_pGEEC529_pShip-CMV-mRuby2-bGH
Plasmid#239725PurposePlasmid expressing mRuby2 with the CMV promoter and bGH 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2498_pGEEC530_pShip-CMV-mRuby2-SV40
Plasmid#239726PurposePlasmid expressing mRuby2 with the CMV promoter and SV40 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1313_pPV3-WPRE
Plasmid#239703PurposeBsaI Golden Gate assembly plasmid encoding the WPRE 3' UTR sequenceDepositorInsertWPRE 3' UTR
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3560_pHarbor-Rogi2_backbone
Plasmid#239706PurposePaqCI Golden Gate assembly plasmid encoding the backbone for Bxb1-mediated integration in the Rogi2 landing pad lineDepositorTypeEmpty backboneUseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1970_pGEEC501_pShip-EFS-mRuby2-bGH
Plasmid#239707PurposePlasmid expressing mRuby2 with the EFS promoter and bGH 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1971_pGEEC502_pShip-EFS-mRuby2-SV40
Plasmid#239708PurposePlasmid expressing mRuby2 with the EFS promoter and SV40 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1972_pGEEC503_pShip-EFS-mRuby2-WPRE
Plasmid#239709PurposePlasmid expressing mRuby2 with the EFS promoter and WPRE 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1973_pGEEC504_pShip-hPGK-mRuby2-bGH
Plasmid#239710PurposePlasmid expressing mRuby2 with the hPGK promoter and bGH 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1974_pGEEC505_pShip-hPGK-mRuby2-SV40
Plasmid#239711PurposePlasmid expressing mRuby2 with the hPGK promoter and SV40 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1975_pGEEC506_pShip-hPGK-mRuby2-WPRE
Plasmid#239712PurposePlasmid expressing mRuby2 with the hPGK promoter and WPRE 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2021_pGEEC507_pShip-UbC-mRuby2-bGH
Plasmid#239713PurposePlasmid expressing mRuby2 with the UbC promoter and bGH 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2022_pGEEC508_pShip-UbC-mRuby2-SV40
Plasmid#239714PurposePlasmid expressing mRuby2 with the UbC promoter and SV40 3' UTRDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1367_pPV1-hPGK
Plasmid#239691PurposeBsaI Golden Gate assembly plasmid encoding the hPGK promoterDepositorInserthPGK promoter
UseSynthetic BiologyMutationRemoval of BsaI cut sitesAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0743_pPV1-ZF43x6-C
Plasmid#239693PurposeBsaI Golden Gate assembly plasmid encoding the COMET ZF43x6-C promoterDepositorInsertZF43x6-C promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3657_pPV1-ZF10.BSx8
Plasmid#239694PurposeBsaI Golden Gate assembly plasmid encoding the synZiFTR ZF10.BSx8 promoterDepositorInsertZF10.BSx8 promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1055_pPV2-tagBFP
Plasmid#239697PurposeBsaI Golden Gate assembly plasmid encoding the tagBFP CDSDepositorInserttagBFP
UseSynthetic BiologyMutationRemoval of BsaI cut sitesAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG3659_pPV2-rtTA-P2A-iRFP720
Plasmid#239698PurposeBsaI Golden Gate assembly plasmid encoding the rtTA-P2A-iRFP720 CDSDepositorInsertrtTA-P2A-iRFP720
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0587_pPV3-bGH
Plasmid#239701PurposeBsaI Golden Gate assembly plasmid encoding the bGH PASDepositorInsertbGH PAS
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0588_pPV3-SV40
Plasmid#239702PurposeBsaI Golden Gate assembly plasmid encoding SV40 PASDepositorInsertSV40 PAS
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2019_pPV1-EF1a
Plasmid#239687PurposeBsaI Golden Gate assembly plasmid encoding the EF1a promoterDepositorInsertEF1a promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG1988_pPV1-UbC
Plasmid#239689PurposeBsaI Golden Gate assemby plasmid encoding the UbC promoterDepositorInsertUbC promoter
UseSynthetic BiologyMutationRemoval of BsaI cut sitesAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSG297-(Sp-gRNA)-(Sa-gRNA)-(PuroR_T2A_BFP(C>T screening))
Plasmid#239448PurposeOrthogonal dual Cas9 gRNA and BFP reporter lentivirus cassette plasmid. For use with S. pyogenes and S. aureus gRNAs and includes a BFP reporter which reports on C-to-T editing.DepositorInsertsS.p. gRNA backbone
S.a. gRNA backbone
PuroR-T2A-BFP(C>T_screening)
UseLentiviralExpressionMammalianMutationBFP: T65S, S72S, V145F, K206K, H231LPromoterEF1alpha, U6, and mU6Available SinceJune 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1176
Plasmid#229871Purposegene trap RMCE plasmid with GFP-C1-tbb-2 3'UTR::SEC for RMCE cloned in the destination vector pHW1133 to swap driver via RMCEDepositorInsertGFP-C1-tbb-2 3'UTR::SEC
TagsGFP-CExpressionWormAvailable SinceJune 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miR.FF5)-TS.FF5x1-bGH
Plasmid#235277PurposeComMAND closed-loop circuit regulating mRuby2 with miR-FF5DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceJune 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mGL-bGH
Plasmid#235299PurposemGreenLantern expression to serve as a transfection marker, EF1a-drivenDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miR.FF4)-TS.FF6x1-bGH
Plasmid#235282PurposeComMAND open-loop circuit regulating mRuby2 with miR-FF4DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
U6-miR.FF4
Plasmid#235256PurposeNon-intronic (U6-driven) expression of miR-FF4 (FF4 hairpin)DepositorInsertFF4 hairpin
UseSynthetic BiologyExpressionMammalianPromoterU6Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EF1a-mRuby2(miRE.FF5)-TS.FF6x1-bGH
Plasmid#235260PurposeComMAND open-loop circuit regulating mRuby2 with miRE-FF5DepositorInsertmRuby2
UseSynthetic BiologyExpressionMammalianPromoterEF1a (human EF1a)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFS-mGL-bGH
Plasmid#235255PurposemGreenLantern expression to serve as a transfection marker, EFS-drivenDepositorInsertmGreenLantern
UseSynthetic BiologyExpressionMammalianPromoterEFS (human EF1a short, intronless)Available SinceMay 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
Hv_HPT:pGstA+WIR1a:Gus_introns
Plasmid#215190PurposeEvaluating guard cell specific promotors in BarleyDepositorInsertGlutathione-S-transferase1 fused with Wheat‐induced resistance1
UseSynthetic BiologyExpressionPlantPromoterTaGstA1 fused with TaWIR1Available SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bo_NPTII:pGstA1+WIRa:Gus_introns
Plasmid#215176PurposeEvaluating guard cell specific promotors in BrassicaDepositorInsertGlutathione-S-transferase1 Wheat‐induced resistance1
UseSynthetic BiologyPromoterTaGstA1 fused with TaWIR1 (chimeric)Available SinceMay 27, 2025AvailabilityAcademic Institutions and Nonprofits only