We narrowed to 10,458 results for: T7

1. pNP1 Aqua toehold switch

   Plasmid

   #107356

   Purpose

   Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   toehold switch 8 + Aquamarine
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEMHE-POLArISact

   Plasmid

   #164970

   Purpose

   T7 promotor drives in vitro transcription of POLArISact with a human kozak sequence

   Depositor

   Sumio Terada

   Article

   Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11

   Insert

   POLArISact
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEMHE-mClover3

   Plasmid

   #171497

   Purpose

   T7 promotor drives in vitro transcription of mClover3 mRNA

   Depositor

   Melina Schuh

   Article

   Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

   Insert

   mClover3
   

   Use

   Pgehme

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-HsSENP6

   Plasmid

   #71469

   Purpose

   Encodes human full length SENP6 optimised for E. coli expression. Suitable for in vitro transcription/translation (T7).

   Depositor

   Jorge Eduardo Azevedo

   Article

   Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

   Insert

   SUMO1/sentrin specific peptidase 6 (SENP6 Human)
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   Gene optimised for E. coli expression (Genscript)

   Promoter

   T7

   Available since

   Dec. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pGEMHE-mEGFP-mTrim21ΔPRYSPRY

   Plasmid

   #105521

   Purpose

   T7 promotor drives in vitro transcription of mEGFP-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

   Depositor

   Melina Schuh

   Article

   Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

   Insert

   TRIM21 (Trim21 Mouse)
   

   Use

   In vitro transcription

   Tags

   mEGFP

   Expression

   Mutation

   deletion of PRYSPRY domain (aa1-281)

   Promoter

   T7

   Available since

   March 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJBL704

   Plasmid

   #140374

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 4BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-4BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJBL705

   Plasmid

   #140375

   Purpose

   Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-5BP-tetO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFAB803

   Plasmid

   #47847

   Purpose

   BIOFAB reporter plasmid for measuring T7 early termination efficiency

   Depositor

   Drew Endy

   Article

   Mutalik et al Nat Methods. 2013 Apr;10(4):354-60. doi:

   Insert

   T7 early terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pLTetO2

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJBL729

   Plasmid

   #140399

   Purpose

   Expresses CadC-regulated 3WJdB RNA aptamer driven by T7 promoter with the cadO sequence 5BP downstream from the promoter

   Depositor

   Julius Lucks

   Article

   Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

   Insert

   T7-cadO-3WJdB-T
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJBL718

    Plasmid

    #140388

    Purpose

    Expresses MobR-regulated 3WJdB RNA aptamer driven by T7 promoter with the mobO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-mobO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJBL712

    Plasmid

    #140382

    Purpose

    Expresses OtrR-regulated 3WJdB RNA aptamer driven by T7 promoter with the otrO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-otrO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMHE-Trim21-OLLAS

    Plasmid

    #237438

    Purpose

    Expresses mouse Trim21 ubiquitin ligase tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

    Insert

    Trim21 (Trim21 Mouse)
    

    Use

    Tags

    OLLAS

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEMHE-MmBorealin-OLLAS

    Plasmid

    #237439

    Purpose

    Expresses mouse Borealin tagged with OLLAS at C-term; made for in vitro transcription (T7 promoter)

    Depositor

    Michael Lampson

    Article

    Dudka et al Nature. 2025 Feb;638(8051):814-822. doi:

    Insert

    Borealin (Cdca8 Mouse)
    

    Use

    Tags

    OLLAS

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. NLuc-His10

    Plasmid

    #234567

    Purpose

    Bacterially expressed NLuc for Ni-NTA purification

    Depositor

    James Inglese

    Article

    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

    Insert

    Nano luciferase
    

    Use

    Tags

    T7 gene 10 leader peptide and Thrombin site-10xHi…

    Expression

    Bacterial

    Mutation

    Codon optimized for bacterial expression

    Promoter

    T7

    Available since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. H75R SUMO1

    Plasmid

    #221060

    Purpose

    Expression of H75R SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    H75R site mutation on SUMO 1 (SUMO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    H75R

    Promoter

    T7

    Available since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-Flag-Jarid2-polyA

    Plasmid

    #223811

    Purpose

    IVT of Flag-Jarid2 mRNA with polyA tail, by the T7 promoter.

    Depositor

    Azusa Inoue

    Article

    Matsuwaka et al Nat Cell Biol. 2025 Jan;27(1):28-38. doi

    Insert

    Jarid2 (Jarid2 Mouse)
    

    Use

    In vitro transcription

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, T7

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. E67K SUMO1

    Plasmid

    #221065

    Purpose

    Expression of E67K SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    E67K site mutation on SUMO 1 (SUMO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E67K

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. H35K SUMO1

    Plasmid

    #221055

    Purpose

    Expression of H35K SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    H35K site mutation on SUMO 1 (SUMO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    H35K

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. H35S SUMO1

    Plasmid

    #221056

    Purpose

    Expression of H35S SUMO1 protein under T7 promoter

    Depositor

    Michael Rosen

    Article

    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

    Insert

    H35S site mutation on SUMO 1 (SUMO1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    H35S

    Promoter

    T7

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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