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Lifeact-7-iRFP670

Plasmid

#103032

Purpose

Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Use

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-GBF1

Plasmid

#206415

Purpose

Mammalian expression of hGBF1

Depositor

Kazuhisa Nakayama

Article

Kawamoto et al Traffic. 2002 Jul;3(7):483-95. doi: 10.1

Insert

GBF1 (GBF1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 c-Fyn

Plasmid

#16032

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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AAVS1-NeoR-CAG-EGFPnls-WPRE

Plasmid

#191692

Purpose

For knocking pCAG-EGFPnls into AAVS1 locus

Depositor

Yuejun Chen

Article

Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

Insert

AAVS1 homology arms and pCAG-EGFPnls

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-LC3-YFP

Plasmid

#99571

Purpose

Lentiviral expression of YFP-tagged LC3

Depositor

Edward Campbell

Article

Flavin et al Acta Neuropathol. 2017 May 19. doi: 10.1

Insert

LC3 (MAP1LC3B Human)

Use

Lentiviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-ihSyn1-tTA

Plasmid

#99120

Purpose

An AAV genome that expresses tTA from the hSyn1 promoter that contains a positive feedback loop for amplifed expression of the tTA

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

tTA

Use

AAV

Tags

Expression

Mammalian

Mutation

*(see below)

Promoter

ihSyn1

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pECE-HA-AMPKbeta1 wt

Plasmid

#31666

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)

Use

Tags

HA (YDVPDYASL)

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-SAKS1-Strep-HA

Plasmid

#113487

Purpose

Expression of human SAKS1 with C-terminal strep-HA tag

Depositor

Hemmo Meyer

Article

Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

Insert

SAKS1 (UBXN1 Human)

Use

Tags

2xstrep-HA

Expression

Mammalian

Mutation

wildtype without stop codon

Promoter

CMV

Available since

Aug. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Cluc-RMA

Plasmid

#189624

Purpose

Expresses Cluc-RMA under the hSyn promoter. Cluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Insert

Cypridina luciferase fused to Fc

Use

AAV and Luciferase

Tags

Cluc and IgG1 Fc

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPong_v2

Plasmid

#47101

Purpose

Expresses the Pong ORF1 and transposase from rice to allow mPing movement. Contains ORF1 and transposase driven by a native soybean promoter. Contains mPing and BAR for basta selection.

Depositor

Wayne Parrott

Article

Hancock et al Plant Physiol. 2011 Oct;157(2):552-62. d

Inserts

mPing
Pong Open Reading Frame 1 (ORF1)
Pong Transposase

Use

Plant expression

Tags

Expression

Mutation

Promoter

GmUbi_AP, GmUbi_XS, and no promoter

Available since

Oct. 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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MCS-TurboID MSCV-IRES-GFP

Plasmid

#207957

Purpose

Retroviral vector for cloning a gene of interest with a glycine-serine linker and TurboID on the C-terminus

Depositor

Timothy Ley

Article

Day et al J Clin Invest. 2023 Dec 7:e176311. doi:

Insert

TurboID

Use

Retroviral; Turboid biotin proximity ligase

Tags

TurboID

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-PBRM1-pgk-puro

Plasmid

#85746

Purpose

tetracyline-inducible lentiviral vector expressing human polybromo-1 (PBRM1) in mammlian cells

Depositor

Emily Dykhuizen

Article

Chowdhury et al PLoS One. 2016 Apr 21;11(4):e0153718. do

Insert

PBRM1 (PBRM1 Human)

Use

Lentiviral

Tags

none

Expression

Mutation

Promoter

CMV

Available since

March 16, 2017

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-ARAF

Plasmid

#51076

Purpose

mammalian expression of wt ARAF

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pBGPa-CMV-GFP-OSBP PH domain

Plasmid

#58724

Purpose

Expresses GFP-Tagged OSBP PH domain in mammalian cells

Depositor

Tim Levine

Article

Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

Insert

Oxysterol-binding Protein PH domain (OSBP Human)

Use

Tags

GFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-GFAP-mKate2.5f

Plasmid

#99129

Purpose

Also known as pAAV-GFAP-mKate2.5-f. An AAV genome that express the farnesylated fluorescent protein mKate2.5 from the GFAP promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mKate2.5f

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

GFAP

Available since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster nanos

Available since

April 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMuLE Lenti Dest eGFP

Plasmid

#62175

Purpose

MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses EGFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Lentiviral; Mule gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5 DN-Fyn

Plasmid

#16033

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Use

Tags

Expression

Mammalian

Mutation

Dominant negative: K299M

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-HA-LATS2-KR

Plasmid

#33100

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

lats2 (Lats2 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

K655R, Kinase Dead

Promoter

CMV

Available since

March 27, 2012

Availability

Academic Institutions and Nonprofits only
pMuLE Lenti Dest iRFP

Plasmid

#62176

Purpose

MuLE (Multiple Lentiviral Expression) Destination vector for use with pMuLE Entry vectors. Co-expresses iRFP under the control of a PGK promoter. Compatible with MultiSite Gateway cloning

Depositor

Ian Frew

Article

Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d

Type

Empty backbone

Use

Lentiviral; Mule gateway destination vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge