We narrowed to 10,632 results for: t7

1. pCDB179

   Plasmid

   #91960

   Purpose

   empty expression vector for 10His-SUMO tag protein purification

   Depositor

   Christopher Bahl

   Type

   Empty backbone

   Tags

   10-His and Smt3

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAcGFP-stop-IVT

   Plasmid

   #205146

   Purpose

   IVT template of AcGFP stop codon between the ORF and MCS and polyA

   Depositor

   Igor Ulitsky

   Insert

   AcGFP1
   

   Use

   In-vitro transcription

   Promoter

   T7

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNIC-CH

   Plasmid

   #26117

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Type

   Empty backbone

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7-lacO

   Available Since

   Sept. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-72B1

   Plasmid

   #59374

   Purpose

   Inducible expression of 71B2 (Q9M156) under T7 promoter

   Depositor

   Christopher Anderson

   Insert

   UGT72B1
   

   Expression

   Bacterial

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNRB65

   Plasmid

   #36455

   Depositor

   Douglas Bishop

   Insert

   ARS501-LEU2 (LEU2 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   Aug. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET12a-HAL

   Plasmid

   #47097

   Depositor

   Marina Ramirez-Alvarado

   Insert

   HAL
   

   Tags

   ompT

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 2, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28b(+)-HP0377 C92A

   Plasmid

   #39335

   Depositor

   SeWon Suh

   Insert

   oxidoreductase
   

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   changed Cysteine 92 to Alanine

   Promoter

   T7

   Available Since

   Sept. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

8. pETM6-E12-vioABECD

   Plasmid

   #66537

   Purpose

   pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with optimized promoters (E12)

   Depositor

   Mattheos Koffas

   Article

   Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

   Inserts

   VioA
   VioB
   VioE
   VioC
   VioD
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Mutant T7 Promoter - G6 and Mutant T7 Promoter - …

   Available Since

   July 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSET-4xTR-TUBE

   Plasmid

   #110312

   Purpose

   E. coli expression and purification of 4xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   4xTR-TUBE (UBQLN1 Human)
   

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pETM6-RgTALsyn

    Plasmid

    #100947

    Purpose

    Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

    Depositor

    Mattheos Koffas

    Article

    Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

    Insert

    RgTALsyn
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Silent mutation to remove internal NdeI site on T…

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRSET-6xTR-TUBE

    Plasmid

    #110313

    Purpose

    E. coli expression and purification of 6xTR-TUBE

    Depositor

    Yasushi Saeki

    Article

    Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

    Insert

    6xTR-TUBE (UBQLN1 Human)
    

    Tags

    N-terminal His6-T7 tag

    Expression

    Bacterial

    Mutation

    All Arg residues in the UBA domain are mutated to…

    Promoter

    T7

    Available Since

    May 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEMHE-mEGFP-Map4

    Plasmid

    #105571

    Purpose

    T7 promotor drives in vitro transcription of mEGFP-tagged Histon MAP4 mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    MAP4 (Map4 Mouse)
    

    Use

    In vitro transcription

    Tags

    mEGFP

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TDH3pro-rtTA-tetO7pro-T7pol-pRS413

    Plasmid

    #239293

    Purpose

    Expresses the reverse tetracycline activator (rtTA) and T7 polymerase; Plasmid #1 of the tet-on overexpression system

    Depositor

    Anita Hopper

    Article

    Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

    Inserts

    T7 polymerase with NLS and P278L
    reverse tetracycline transactivator (rtTA)
    

    Expression

    Yeast

    Mutation

    Contains M2-SE-G72P mutations, described in Addge…

    Promoter

    TDH3 and tetracycline-responsive element (TRE) an…

    Available Since

    June 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFloat.T7_v1

    Plasmid

    #133070

    Purpose

    Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

    Depositor

    David Sabatini, Kacper Rogala

    Article

    Rogala et al Science. 2019 Oct 25;366(6464):468-475.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    3C-His6 and His6-3C

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJBL703

    Plasmid

    #140373

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-2BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. SSB-wt

    Plasmid

    #197260

    Purpose

    wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

    Depositor

    Timothy Lohman

    Article

    Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

    Insert

    wt E. coli SSB
    

    Tags

    none

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

    Plasmid

    #130640

    Purpose

    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6
    

    Use

    CRISPR; Transposon

    Tags

    His10-MBP-TEVsite on TniQ

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMHE-mCherry-mTrim21ΔPRYSPRY

    Plasmid

    #105524

    Purpose

    T7 promotor drives in vitro transcription of mCherry-tagged mouse TRIM21 with deleted PRYSPRY domain mRNA

    Depositor

    Melina Schuh

    Article

    Clift et al Cell. 2017 Nov 13. pii: S0092-8674(17)31

    Insert

    TRIM21 (Trim21 Mouse)
    

    Use

    In vitro transcription

    Tags

    mCherry

    Mutation

    deletion of PRYSPRY domain (aa1-281)

    Promoter

    T7

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-SARS-CoV-2 NSP13

    Plasmid

    #176492

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

    Depositor

    Xiaodong Cheng

    Article

    White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

    Insert

    SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)
    

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    I570 from SARS-CoV NSP13 was mutated to V to conv…

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pJBL728

    Plasmid

    #140398

    Purpose

    Expresses CsoR-regulated 3WJdB RNA aptamer driven by T7 promoter with the csoO sequence 5BP downstream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-csoO-3WJdB-T
    

    Use

    Synthetic Biology

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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