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We narrowed to 5,023 results for: cre encoding plasmid

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Showing: 2441 - 2480 of 5023 results

  11. pZ3CH10-donor

    Plasmid
    #176368
    Purpose
    CRISPR donor plasmid to create GFP fusion proteins
    Depositor
    Barbara Stranger, Kevin White
    Insert
    Z3CH10
    Use
    CRISPR
    Tags
    GFP
    Available Since
    Nov. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    176368-plasmid-map-sequence-id-347864

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  12. pET-28b-T7-henAsCas12a(E174R/S542R/K548R)-NLS(nucleoplasmin)-6xHis (AAS1885)

    Plasmid
    #114072
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized enAsCas12a(E174R/S542R/K548R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R, S542R, and K548R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114072-plasmid-map-sequence-id-220522

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  13. pCAG-hAsCas12a-enRVR(E174R/S542R/K548V/N552R)-NLS(nucleoplasmin)-3xHA (AAS1060)

    Plasmid
    #114093
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-enRVR(E174R/S542R/K548V/N552R) nuclease with C-terminal NLS and HA tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRVR (E174R/S542R/K548V/N552R)
    Tags
    NLS(nucleoplasmin) and 3xHA
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114093-plasmid-map-sequence-id-230492

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  14. pET-28b-T7-hAsCas12a-enRR(E174R/S542R/K607R)-NLS(nucleoplasmin)-6xHis (AAS1902)

    Plasmid
    #114077
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-enRR(E174R/S542R/K607R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-enRR (E174R/S542R/K607R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    E174R/S542R/K607R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114077-plasmid-map-sequence-id-220525

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  15. pCAG-rAPOBEC1-gs-XTEN-gs-hdenAsCas12a(E174R/S542R/K548R/D908A)-gs-UGI-NLS(SV40)(enAsBE1.3) (RTW1296)

    Plasmid
    #114083
    Purpose
    CAG promoter expression plasmid for human codon optimized enAsBE1.3
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to N-terminal rAPOBEC1 and C-terminal UGI (enAsBE1.3)
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A, human codon optimi…
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114083-plasmid-map-sequence-id-220558

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  16. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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  19. MADR pDonor-H3F3A-K27M-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131462
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A K27M, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-K27M-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A K27M, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131462-plasmid-map-sequence-id-305183

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  20. MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131461
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    131461-plasmid-map-sequence-id-305184

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  21. MADR pDonor-H3F3A-G34R-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

    Plasmid
    #131463
    Purpose
    MADR donor plasmid for FlpO+Cre-mediated insertion of oncogenic H3F3A G34R, PDGFRA D842V, and TRP53
    Depositor
    Joshua Breunig
    Article
    Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do
    Insert
    H3F3A-G34R-EGFP AU1 pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE
    Use
    Cre/Lox and Synthetic Biology; Mosaic analysis fo…
    Tags
    Trp53-V5, H3F3A-EGFP-AU1
    Expression
    Mammalian
    Mutation
    H3F3A G34R, Pdgfra D842V
    Promoter
    none (4x polyA to mitigate episomal expression)
    Available Since
    July 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    131463-plasmid-map-sequence-id-305001

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  31. pBHSTC104

    Plasmid
    #124356
    Purpose
    Plasmid expressing N- and C-terminal split fragments of gp41-1 intein inserted into CcdA antitoxin
    Depositor
    Hideo Iwai
    Article
    Beyer et al Chembiochem. 2019 Apr 8. doi: 10.1002/cb
    Insert
    gp41-1 N-intein and C-intein (bicistronic)
    Tags
    CcdA-C (SFAD)
    Expression
    Bacterial
    Promoter
    STC
    Available Since
    April 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    124356-plasmid-map-sequence-id-242355

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  38. Venus-Bik-del72-136-pEGFP-C3

    Plasmid
    #191411
    Purpose
    Transfection of this plasmid will express "VBik-d72-136" (Venus fused to the N-terminus of the BH3-only protein, Bik that has amino acids 72-136 deleted).
    Depositor
    David Andrews
    Insert
    Bcl-2-interacting killer (BIK Human)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    with deletion of amino acids 72-136 (del72-136) i…
    Promoter
    CMV
    Available Since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    191411-plasmid-map-sequence-id-376884

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  39. pRNF219.1.0-gDNA

    Plasmid
    #132464
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    RNF219
    Use
    CRISPR
    Available Since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132464-plasmid-map-sequence-id-267084

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Showing: 2441 - 2480 of 5023 results