Addgene

We narrowed to 4,286 results for: ARA-2

Showing: 1221 - 1240 of 4286 results

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p12x601

Plasmid

#157785

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backbone

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

pMD2.G(spei/spei)&12x601_tetO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMM113

Plasmid

#127210

Purpose

BeYDV replicon with constitutive Luc expression, WUS2 and AtSTM, sgRNA targeting PDS

Depositor

Daniel Voytas

Article

Maher et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

WUS2, AtSTM, sgRNA, Luc

Use

Tags

Expression

Plant

Mutation

codon optimized CDSs

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFSynWSyt1C2AB(80-421)msGFP

Plasmid

#209014

Purpose

Overexpression of truncated syt1 tagged with GFP in mammalian cells

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Use

Lentiviral

Tags

msfGFP

Expression

Mammalian

Mutation

aa 80-421 only

Promoter

Available since

Nov. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pFW2000

Plasmid

#224211

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW2100

Plasmid

#224212

Purpose

Bacteroides genomic editing tool; IPTG inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSLS.491

Plasmid

#184956

Purpose

Test effect of a1/a2 length on editing rpoB using retron recombineering

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 RT and recombineering ncRNA, rpoB T1534C, a1/a2 length: 12

Use

Tags

Expression

Bacterial

Mutation

rpoB donor T1534C

Promoter

T7/lac

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pFW5000

Plasmid

#224214

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin), tetQ

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pFW6000

Plasmid

#224215

Purpose

Bacteroides genomic editing tool; aTc inducible-Fncpf1

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Insert

Fncpf1

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAcGFP1-N1-MAHS

Plasmid

#90034

Purpose

To express GFP-tagged MAHS in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

MAHS

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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Fragment 1 Null

Plasmid

#198729

Purpose

Gateway-compatible gRNA backbone . Template for the addition of target sequences to produce 2x gRNA.

Depositor

Friedrich Kragler

Article

Yang et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

gRNA expression cassette in pENTR4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pSCL.139

Plasmid

#184994

Purpose

Express Cas9-P2A-Eco1RT in human cells

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

SpCas9-P2A-Eco1RT

Use

Tags

SV40NLS

Expression

Mammalian

Mutation

Human codon optimized RT

Promoter

TetOn-3g

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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Osa-pCAMBIA-1301-STTM319

Plasmid

#84201

Purpose

Disturb the expression of miR319 in rice

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR319

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo5

Plasmid

#157804

Purpose

Expression of synthetic protein composed of eGFP and 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo5

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Gma-pCAMBIA-1301-STTM171

Plasmid

#84080

Purpose

Disturb the expression of miR171 in soybean

Depositor

Guiliang Tang

Article

Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

Insert

miR171

Use

Tags

Expression

Bacterial, Plant, and Yeast…

Mutation

Promoter

2x35S

Available since

Sept. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pMTTH+eGFP-bromo1

Plasmid

#157805

Purpose

Expression of synthetic protein composed of eGFP and BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

eGFP-bromo1

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGENE:EFF-1::mCherry::V5

Plasmid

#133020

Purpose

EFF-1 fused to mCherry fluorescent protein cloned into the pGene vector for inducible expression in mammalian cells

Depositor

Benjamin Podbilewicz

Article

Perez-Vargas et al Cell. 2014 Apr 10;157(2):407-419. doi: 1

Insert

EFF-1::mCherry::V5

Use

Tags

V5; mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2019

Availability

Academic Institutions and Nonprofits only

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Grp1-PH pET11a

Plasmid

#71377

Purpose

Phosphoinositide Binding

Depositor

Mark Lemmon

Article

Ferguson et al Mol Cell. 2000 Aug;6(2):373-84.

Insert

Grp1-PH (Cyth3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Pleckstrin homology (PH) domain; aa 264-391

Promoter

T7

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAcGFP1-N1-RvLEAM

Plasmid

#90035

Purpose

To express GFP-tagged RvLEAM in mammalian cells transiently

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

Insert

RvLEAM

Use

Tags

AcGFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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