We narrowed to 2,341 results for: PUC19

1. qTAG-C-mStayGold-Zeo

   Plasmid

   #227255

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Zeo cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-V5-Puro

   Plasmid

   #207730

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Puro cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pADH99

   Plasmid

   #90979

   Purpose

   NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Inserts

   US_HIS1
   FRT
   pENO1
   C. albicans Cas9
   pMAL2
   FLP
   NAT 1 of 2
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCap-SG5-Hgy

   Plasmid

   #227580

   Purpose

   Capture plasmid with hygromycin resistance and pSG5 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pADH100

   Plasmid

   #90980

   Purpose

   NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pADH147

   Plasmid

   #90991

   Purpose

   NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.

   Depositor

   Aaron Hernday

   Article

   Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi

   Insert

   gRNA conserved, FRT, DS_HIS1
   

   Use

   CRISPR

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. qTAG-C-3xFLAG-Blast

   Plasmid

   #207717

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. qTAG-N-Zeo-mStayGold

   Plasmid

   #227252

   Purpose

   Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mStayGold cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pN7CAST

   Plasmid

   #190661

   Purpose

   Expresses polycistronic N7 CAST where Cas12k-T2A-TnsC and TniQ-T2A-TnsB are separated by an EMCV IRES

   Depositor

   Benjamin Kleinstiver

   Article

   Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   human codon optimized N7Cas12k, N7TnsC, N7TniQ, N7TnsB
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-3xFLAG-Zeo

    Plasmid

    #207739

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. qTAG-C-V5-Blast

    Plasmid

    #207719

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. qTAG-C-V5-Zeo

    Plasmid

    #207741

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a V5-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pC0042-PguCas13b crRNA backbone

    Plasmid

    #103853

    Purpose

    For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Insert

    U6-PguCas13b DR-BbsI-BbsI-polyT
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. qTAG-C-mScarlet-Puro

    Plasmid

    #207722

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. qTAG-KO-PGK-Puro

    Plasmid

    #227264

    Purpose

    Empty selectable Knock-Out donor cassette. Integrate homology arms to target the N-terminus insertion of a PGK-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-N-Blast-ultraID

    Plasmid

    #207682

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pEF1α-Cre-2A-moxGFP

    Plasmid

    #207744

    Purpose

    Expression of the Cre recombinase linked to a moxGFP fluorescent marker

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    Cre-PGK-moxGFP
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pDD282

    Plasmid

    #66823

    Purpose

    GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

    Insert

    GFP-C1^SEC^3xFlag
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans codon-optimized GFP

    Expression

    Worm

    Available Since

    June 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-dTAG-Zeo

    Plasmid

    #207738

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pT2SC

    Plasmid

    #59382

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat
    

    Use

    Template

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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