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pCR8-DJ1-D149A
Plasmid#29355DepositorInsertDJ1 (PARK7 Human)
UseEntry vectorTagsExpressionMutationAsp149AlaPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ER-ExRai-CKAR2
Plasmid#236099PurposeEnhanced excitation-ratiometric biosensor for monitoring Protein Kinase C activity in living cells, targeted to ER surface.DepositorInsertCYP450-ExRai-CKAR2
UseTagsN-terminal 27 amino acids from CYP450ExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-shortSUMO1
Plasmid#228951Purposeexpress a fragment of the human SUMO1 protein (residues 18-97 of UniProt P6316 with an N-terminal thrombin-cleavable tag, MGSSHHHHHHSSGLVPR|GSHM-, where | indicates the cleavage site). Codon-optimizedDepositorInsertSUMO1 (SUMO1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationdeleted amino acids 1-17PromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-RANGAP1-CTD
Plasmid#228947Purposeexpress the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimizedDepositorInsertRANGAP1-CTD (RANGAP1 Human)
UseTagsHis tag and thrombine cleavage site : MGSSHHHHHHS…ExpressionBacterialMutationcontains aa 418-587 onlyPromoterT7-lacAvailable sinceMay 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-FlpX-mKate2.0-N2cG
Plasmid#234711PurposepAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linkerDepositorInsertmKate2.0 T2A N2cG
UseAAVTagsT2AExpressionMammalianMutationPromoterCAGAvailable sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-roxSTOProx-NaChBac-TdTomato
Plasmid#236253PurposeDre dependent NaChBac-TdTomato expressionDepositorInsertNaChBac-TdTomato
UseAAVTagsTdTomatoExpressionMammalianMutationPromoterhSynAvailable sinceApril 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO_24xGCN4_PCC_24xPP7
Plasmid#236094PurposeConstitutively encodes 24xGCN4-PCC-24xPP7 stem loops, for visualization of the Pcc mRNA.DepositorInsert24xGCN4-PCC-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4TO_24xGCN4_MUT_24xPP7
Plasmid#236093PurposeConstitutively encodes 24xGCN4-MUT-24xPP7 stem loops, for visualization of the Mut mRNA.DepositorInsert24xGCN4-MUT-24xPP7
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_GFP
Plasmid#235611PurposeEncodes GFP. For use in transfections as a negative control without epitope tags.DepositorInsertGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN_NES_V5_GFP
Plasmid#235632PurposeEncodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.DepositorInsertGFP
UseTagsNES-V5ExpressionMammalianMutationPromoterAvailable sinceApril 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCMV_Myc-HalM-P2A-FLAG-YFast-TEV-His-HalB
Plasmid#225064PurposeExpression of HalM-modified HalA2 in human cellsDepositorInsertHalA2
UseTagsMultiple tags due to polycistronic expressionExpressionMammalianMutationWTPromoterCMVAvailable sinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Key
Plasmid#216503PurposeRas activity dependent proximity labeler, Key only, untargetedDepositorInsertRas-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Cage
Plasmid#216502PurposeRas activity dependent proximity labeler, Cage only, untargetedDepositorInsertRas-LOCKR-PL Cage
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PM-Ras-LOCKR-PL Key
Plasmid#216504PurposeRas activity dependent proximity labeler, Key only, plasma membrane localizedDepositorInsertPM-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Golgi-Ras-LOCKR-PL Key
Plasmid#216505PurposeRas activity dependent proximity labeler, Key only, Golgi localizedDepositorInsertGolgi-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 ER-Ras-LOCKR-PL Key
Plasmid#216506PurposeRas activity dependent proximity labeler, Key only, ER localizedDepositorInsertER-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito-Ras-LOCKR-PL Key
Plasmid#216507PurposeRas activity dependent proximity labeler, Key only, mitochondria localizedDepositorInsertMito-Ras-LOCKR-PL
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pVRC-N-Venus-V150A-apCC-Di
Plasmid#190706PurposeEncodes a modified version (V150A) of N-Venus followed by the antiparallel coiled-coil apCC-Di.DepositorInsertN-Venus-V150A-apCC-Di
UseTagsExpressionMutationV150APromoteraraBADAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSub-Asp6-MBP-HRas
Plasmid#185758PurposeBacterial expression of Asp6-MBP-HRasDepositorInsertAsp6-MBP-HRas
UseTagsMBPExpressionBacterialMutation6x Asp added to the N terminus of MBPPromotertacAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only
