We narrowed to 6,905 results for: crispr cas9 plasmids

1. pFREE2-eSpCas9-sgRNA

   Plasmid

   #179580

   Purpose

   Expresses eSpCas9 and gRNA that targets ColE1 plasmids for curing

   Depositor

   Kevin Solomon

   Article

   Lee et al ACS Synth Biol. 2022 Jan 21;11(1):53-60.

   Insert

   eSpCas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSpCas9(BB)-2A-Puro-SMCR8

   Plasmid

   #83440

   Purpose

   Expresses Cas9 and a gRNA targeting SMCR8

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human SMCR8 (SMCR8 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX602-AAV-TBG::NLS-SaCas9-NLS-HA-OLLAS-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61593

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   HA, NLS, and OLLAS tag

   Expression

   Mammalian

   Mutation

   K175R and K736R (deUb mutations)

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS3-3xFLAG-2xNLS

   Plasmid

   #107300

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS3 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP
   

   Use

   CRISPR

   Tags

   3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-ZFPTS2-3xFLAG-2xNLS

   Plasmid

   #107299

   Purpose

   Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and ZFP-VEGFA-TS2 in mammalian cells

   Depositor

   Scot Wolfe

   Insert

   Truncated SpCas9 (aa 574-1368) fused to FKBP
   

   Use

   CRISPR

   Tags

   3x Flag, 2x NLS, FKBP, SV40NLS, 3xHA, c-Myc NLS, …

   Expression

   Mammalian

   Mutation

   Truncated SpCas9 (aa 574-1368) with R1335K mutati…

   Promoter

   CMV IE94

   Available since

   Nov. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS

   Plasmid

   #121821

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-dCas9 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. IH601: pMAGIC (R4-R3) NLS-Sa Cas9-NLS

   Plasmid

   #121827

   Purpose

   pMAGIC R4-R3 entry plasmid, contains 2x NLS SaCas9 (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Insert

   Sa-Cas9 (open)
   

   Use

   Synthetic Biology; Pmagic gateway entry plasmid

   Tags

   NLS

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBL1401 pLenti-pEF1a(short)-mNeonGreen-P2A-Cas9

   Plasmid

   #163644

   Purpose

   Makes a lentivirus that expresses mNeonGreen and Cas9.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Insert

   mNeonGreen-P2A-Cas9
   

   Use

   Lentiviral

   Tags

   mNeonGreen-P2A

   Expression

   Mutation

   Promoter

   EFS

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBK1301-AAV-EFSNC-dCjCas9-NIPP1(143-224)

   Plasmid

   #223146

   Purpose

   Expression of truncated NIPP1 with dCjCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   NIPP1 (PPP1R8 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 143-224

   Promoter

   EF1a

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBK1300-AAV-EFSNC-dCjCas9-MBD2(176-231)

    Plasmid

    #223145

    Purpose

    Expression of truncated MBD2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD2 (MBD2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 176-231

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBK1299-AAV-EFSNC-dCjCas9-MBD1(529-592)

    Plasmid

    #223144

    Purpose

    Expression of truncated MBD1 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD1 (MBD1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 529-592

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBK1306-AAV-EFSNC-dSaCas9-HP1aNH(115-177)

    Plasmid

    #223151

    Purpose

    Expression of truncated HP1a with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1a (CBX5 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 115-177

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBK1296-AAV-EFSNC-dCjCas9-HP1aNH(115-177)

    Plasmid

    #223141

    Purpose

    Expression of truncated HP1a with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    HP1a (CBX5 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 115-177

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBK1309-AAV-EFSNC-dSaCas9-MBD1(529-592)

    Plasmid

    #223154

    Purpose

    Expression of truncated MBD1 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD1 (MBD1 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 529-592

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBK1311-AAV-EFSNC-dSaCas9-NIPP1(143-224)

    Plasmid

    #223156

    Purpose

    Expression of truncated NIPP1 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    NIPP1 (PPP1R8 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 143-224

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBK1310-AAV-EFSNC-dSaCas9-MBD2(176-231)

    Plasmid

    #223155

    Purpose

    Expression of truncated MBD2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    MBD2 (MBD2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 176-231

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. L40C-CRISPR.EFS.PAC

    Plasmid

    #89393

    Purpose

    Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBK1314-AAV-EFSNC-dSaCas9-KRAB-MECP2

    Plasmid

    #223159

    Purpose

    Expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

    Plasmid

    #223149

    Purpose

    Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-3xsgRNA_Opn1mw-RHO-Cas9N-IntN-polyA

    Plasmid

    #165450

    Purpose

    Express N-terminal part of split dCas9-VPR and 3 sgRNAs targeting murine Opn1mw promoter

    Depositor

    Elvir Becirovic

    Article

    Bohm et al Sci Adv. 2020 Aug 19;6(34):eaba5614. doi

    Inserts

    dCas9N-IntN
    3x Opn1mw promoter-targeting sgRNAs
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and short human rhodopsin promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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