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R777-E163 Hs.PIK3CA

Plasmid

#70447

Purpose

Gateway ORF clone of human PIK3CA [NM_006218.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

PIK3CA (PIK3CA Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCONR221

Plasmid

#216146

Purpose

Gateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistance

Depositor

Imran Siddiqi

Article

Sivakumar et al Physiol Mol Biol Plants. 2024 Jul;30(7):

Type

Empty backbone

Use

Cloning backbone

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pEB1-T-Sapphire

Plasmid

#103977

Purpose

Plasmid encoding T-Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

T-Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (Q69M, C70V, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-mOrange

Plasmid

#102632

Purpose

In vitro expression of mOrange from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mOrange

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12V

Plasmid

#179654

Purpose

KRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12V

Expression

Mammalian

Mutation

G12V

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-TARG1

Plasmid

#172594

Purpose

For purification (from E.coli) of His-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

His

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_ATP1.0

Plasmid

#167579

Purpose

Express the ATP sensor GRAB_ATP1.0 in astrocytes

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Use

AAV

Promoter

GfaABC1D

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pSL1777 (pEffector)

Plasmid

#160731

Purpose

Effector expression for V. cholerae CAST system, encodes all protein and RNA components. Entry vector encodes non-targeting crRNA, with BsaI sites for spacer cloning. pCDF backbone.

Depositor

Samuel H. Sternberg

Article

Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103

Insert

VchCAST proteins and crRNA

Expression

Bacterial

Promoter

J23119

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A38

Plasmid

#132053

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A38 (SLC25A38 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDN42

Plasmid

#61345

Purpose

mCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)

Depositor

Barbara Di Ventura, Roland Eils

Article

Niopek et al Nat Commun. 2014 Jul 14;5:4404. doi: 10.

Insert

mCherry

Tags

GS linker-AsLov2-biNLS10 and PKIt NES

Expression

Mammalian

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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