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pRK5 FLAG membrane-bound lipin-1 (TMD)

Plasmid

#120277

Purpose

Expression of ER/Golgi-tethered lipin-1 in mammalian cells

Depositor

Sirio Dupont

Article

Romani et al Nat Cell Biol. 2019 Mar;21(3):338-347. d

Insert

Lipin-1 (Lpin1 Mouse)

Use

Tags

FLAG and in-frame fusion with the TMD Golgi-local…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 LYCHOS (GPR155) ∆Permease-HA

Plasmid

#199686

Purpose

Transient expression of LYCHOS (GPR155) ∆Permease

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion from amino acid 1-369

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 LYCHOS ∆LED-HA

Plasmid

#199682

Purpose

Transient expression of LYCHOS (GPR155) ∆LED-HA

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion from amino acid 556-651

Promoter

CMV

Available since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-LYCHOS (GPR155) FP>IA-HA

Plasmid

#199661

Purpose

Transient expression of LYCHOS (GPR155) FP>IA mutant

Depositor

Roberto Zoncu

Article

Shin et al Science. 2022 Sep 16;377(6612):1290-1298

Insert

GPR155 (GPR155 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

F43 and P44 mutated to I and A

Promoter

CMV

Available since

April 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF-Coff/Fon-bREACHes-EYFP

Plasmid

#137147

Purpose

Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

Depositor

INTRSECT 2.0 Project, Karl Deisseroth

Article

Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

Insert

Coff/Fon-bREACHes-EYFP

Use

AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

Tags

Expression

Mutation

n/a

Promoter

nEF

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-TrpC5 [N67/15R]

Plasmid

#182118

Purpose

Mammalian Expression Plasmid of anti-TrpC5 (Human). Derived from hybridoma N67/15.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpC5 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC5 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-TrpV3 [N15/39R]

Plasmid

#188210

Purpose

Mammalian Expression Plasmid of anti-TrpV3 (Rat). Derived from hybridoma N15/39

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpV3 (Rattus norvegicus) recombinant mouse monoclonal antibody (Trpv3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Nav1.8 Na+ channel scFv [N134/12]

Plasmid

#190531

Purpose

Mammalian Expression of Nav1.8 Na+ channel scFV. Derived from hybridoma N134/12.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Nav1.8 Na+ channel (Rattus norvegicus) recombinant scFV (Scn10a Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-7 [N275/14R]

Plasmid

#177526

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-7 (Mouse). Derived from hybridoma N275/14.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Synaptotagmin-7 (Mus musculus) recombinant mouse monoclonal antibody (Syt7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-EVA1ext-hFcIgG3-IRES-YFP

Plasmid

#64926

Purpose

To express extracellular portion of EVA1 (mouse) fused with human Fc(IgG3)

Depositor

Pau Serra

Article

Garabatos et al Monoclon Antib Immunodiagn Immunother. 2

Insert

mouse EVA1 fused with human FcIgG3 (Mpzl2, IGHG3 Human, Mouse)

Use

Retroviral

Tags

IRES-YFP

Expression

Mammalian

Mutation

Promoter

LTR

Available since

June 5, 2015

Availability

Academic Institutions and Nonprofits only