2,226 results

1. pCAG-lox-inv[Talpha1-iCre-pA]-lox-Nedd1-mOrange2-rCM1

   Plasmid

   #196879

   Purpose

   Neuron-specific expression of Need1 fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Nedd1-mOrange2-rCM1 (Nedd1 Rat)
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Quimeric CAG

   Available since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Kv1.4/RBG4

   Plasmid

   #196938

   Purpose

   Plasmid expressing an antigen targeting the Kv1.4 potassium channel subunit

   Depositor

   James Trimmer

   Insert

   Kv1.4 (Kcna4 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Kv1.2/RBG4

   Plasmid

   #196941

   Purpose

   Plasmid expressing an antigen targeting the Kv1.2 potassium channel subunit

   Depositor

   James Trimmer

   Insert

   Kv1.2 (Kcna2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Kv1.1/RBG4

   Plasmid

   #196943

   Purpose

   Plasmid expressing an antigen targeting the Kv1.1 potassium channel subunit

   Depositor

   James Trimmer

   Insert

   Kv1.1 (Kcna1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. KChIP2/RBG4; 9QM3/RBG4

   Plasmid

   #196944

   Purpose

   Plasmid expressing an antigen targeting the KChIP2b potassium channel subunit

   Depositor

   James Trimmer

   Insert

   KChIP2 (Kcnip2 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Kv1.5/RBG4

   Plasmid

   #196945

   Purpose

   Plasmid expressing an antigen targeting the Kv1.5 potassium voltage-gated channel subunit

   Depositor

   James Trimmer

   Insert

   Kv1.5 (Kcna5 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Kv1.3/RBG4

   Plasmid

   #196949

   Purpose

   Plasmid expressing an antigen targeting the Kv1.3 potassium channel subunit

   Depositor

   James Trimmer

   Insert

   Kv1.3 (Kcna3 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Rat MMP-9 pcDNA3.1

   Plasmid

   #196951

   Purpose

   Plasmid expressing an antigen targeting the MMP9 metalloproteinase

   Depositor

   James Trimmer

   Insert

   mmp-9 (Mmp9 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. cacnb1b pcDNA3

   Plasmid

   #206105

   Purpose

   expression of rat beta1b calcium channel auxillary subunit

   Depositor

   Annette Dolphin

   Insert

   cacnb1b (Cacnb1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. cacna2d1 BBS pMT2

    Plasmid

    #206062

    Purpose

    expression of rat calcium channel alpha2delta-1 with a single Bungarotoxin Binding Site motif inserted after aa549

    Depositor

    Annette Dolphin

    Article

    Meyer et al Sci Rep. 2021 May 13;11(1):10256. doi: 1

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. cacna2d1 HA D491A pcDNA3

    Plasmid

    #206070

    Purpose

    expression of rat calcium channel alpha2delta-1 with a single HA tag inserted after aa549 and a D491A mutation that prevents Gabapentin binding

    Depositor

    Annette Dolphin

    Article

    Gumerov et al Proc Natl Acad Sci U S A. 2022 Mar 8;119

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D491A

    Promoter

    CMV

    Available since

    Sept. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGWB511-TP-BVRA

    Plasmid

    #203480

    Purpose

    For Agrobacterium transformation. Rattus rattus biliverdin reductase A (BVRA) flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag under the control of the 35S promoter.

    Depositor

    Yutaka Kodama

    Article

    Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

    Insert

    Rattus rattus biliverdin reductase A flanked with an N-terminal plastid transit peptide and a C-terminal FLAG tag.
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGL3-MTS-ND1-W86-R17-G1333N-UGI-Puro

    Plasmid

    #198851

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND1) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND1-W86-R17 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pEGFPC2-gephyrin S268E/270E

    Plasmid

    #199609

    Purpose

    Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S268E/S270E

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFsynW SYT1-PGM reporter

    Plasmid

    #197278

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) for use in the RUSH system; visualized with HaloTag. Reporter only (no hook). SYT1 is mutated to disrupt palmitoylation and glycosylation.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    T15A, T16A, N24Q, C74A, C75A, C77A, C79A, and C82A

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFsynW SYT1deltaC2AB reporter

    Plasmid

    #197279

    Purpose

    Encodes for viral expression of Synaptotagmin 1 (SYT1) (1 - 140 a.a.) to synchronize its transport from the ER (RUSH system); visualized with HaloTag. Reporter only (no hook).

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYT1 (1 - 420 a.a.) and HaloTag (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    hSyn (human synapsin I promoter)

    Available since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GluR1-Flop/pRK5

    Plasmid

    #197308

    Purpose

    Plasmid expressing an antigen targeting the GluR1/GluA1 AMPA receptor subunit

    Depositor

    Robert Wenthold

    Insert

    GluR1-Flop (Gria1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Kv1.4/GW1

    Plasmid

    #197331

    Purpose

    Plasmid expressing an antigen targeting the Kv1.4 potassium channel subunit

    Depositor

    Morgan Sheng

    Insert

    Kv1.4 (Kcna4 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-lox-inv[Talpha1-iCre-pA]-lox-2xHA-128-425-Akap9

    Plasmid

    #196897

    Purpose

    Neuron-specific expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to 2xHA-tags.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    2xHA-Akap9(128-425) (Akap9 Rat)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBactin-Nedd1-mOrange2

    Plasmid

    #196860

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2 (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBactin-AcGFP-Cdk5rap2-CTDdeltaCBD

    Plasmid

    #196856

    Purpose

    Expression of the centrosome targeting carboxy-terminal domain (CTD) laking the centrosome binding domain (deltaCBD) of Cdk5rap2 fused to AcGFP. It cannot displace Cdk5rap2 from centrosomes

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    AcGFP-Cdk5rap2-CTDdeltaCBD (Cdk5rap2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Lacking the centrosome-targeting domain (CTD)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBactin-Nedd1-mOrange2-rCM1

    Plasmid

    #196861

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and rat (r) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-rCM1 (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBactin-Nedd1-mOrange2-F75A

    Plasmid

    #196862

    Purpose

    Expression of Need1-rCM1 harboring a (F75A) mutation in the rat (r) Centrosomin motif 1 (CM1) and fused to mOrange2. F75A mutatuon renders CM1 incapable of binding and activating gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-F75A (Nedd1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    F75A in the CM1 domain

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCI pHluorin Syt17

    Plasmid

    #184514

    Purpose

    Mammalian expression of Synaptotagmin 17 with pH-sensitive fluorescent protein

    Depositor

    Edwin Chapman

    Article

    Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

    Insert

    Synaptotagmin 17 (Syt17 Rat)
    

    Use

    Tags

    Super Ecliptic pHluorin

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. FUGW-APOBEC1-YTH-mCherry

    Plasmid

    #197035

    Purpose

    Express APOBEC1 cytosine deaminase fused with YTH m6A binding domain from UbiC promoter.

    Depositor

    Ronald Hart

    Insert

    APOBEC1-YTH
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEGFPC2-gephyrin C3

    Plasmid

    #199610

    Purpose

    Encodes N-terminal eGFP-tagged gephyrin including the C3 splice cassette for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C3 cassette insertion

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEGFPC2-gephyrin C4a

    Plasmid

    #199611

    Purpose

    Encodes N-terminal eGFP-tagged gephyrin including the C4a splice cassette for expression in mammalian cells.

    Depositor

    Shiva Tyagarajan

    Article

    Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

    Insert

    Gephyrin (Gphn Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    C4a casette insertion

    Promoter

    CMV

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL3-MTS-ND6-W82-R15-G1333C-UGI-Puro

    Plasmid

    #198861

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND6) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND6-W82-R15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL3-MTS-ND5-W68-R15-G1333N-UGI-Puro

    Plasmid

    #198859

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND5) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND5-W68-R15 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-MTS-ND4-W24-R17-G1333N-UGI-Puro

    Plasmid

    #198857

    Purpose

    Employ DdCBE-mediated mtDNA editing to ablate mtProtein(ND4) by swapping Trp codon TGA with stop codon TAA in C6 cells

    Depositor

    Bin Shen

    Article

    Tan et al Sci Adv. 2023 Apr 14;9(15):eadf2695. doi

    Insert

    ND4-W24-R17 TALEN
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge