We narrowed to 5,049 results for: golden gate
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Plasmid#221978PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 DdCBE C2
Plasmid#221979PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 DdCBE T1
Plasmid#221980PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 DdCBE T2
Plasmid#221981PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE G1
Plasmid#221982PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE C2
Plasmid#221983PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE T1
Plasmid#221984PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE T2
Plasmid#221985PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable SinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJJB1577
Plasmid#218619PurposeExpress Pex5, Pex2, Pex17, and Pex10DepositorInsertPex5
ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRO174
Plasmid#233809PurposeMoClo-YTK; type3A; ABA systems; Phlf-NESDepositorInsertPhlf-NES
ExpressionYeastAvailable SinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQ135A
Plasmid#69277PurposeGolden Gate entry vector; 5th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ134-tRNA
Plasmid#179213PurposeGolden Gate entry vector; 4th gRNA with tRNADepositorTypeEmpty backboneUseCRISPRPromoterno promoterAvailable SinceMay 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ136A
Plasmid#69278PurposeGolden Gate entry vector; 6th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD536 VP16-ZF150
Plasmid#138769PurposeConstitutive expression of VP16-ZF150 under the CMV promoter.DepositorInsertVP16-ZF150
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD538 VP16-ZF172
Plasmid#138772PurposeConstitutive expression of VP16-ZF172 under the CMV promoter.DepositorInsertVP16-ZF172
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSN007
Plasmid#102440PurposeFor PCR amplification to create paired gRNAs to clone into Golden Gate gRNA expression vectors (e.g. pSB700 Plasmid #64046)DepositorInsert7SK paired gRNA insert
UseSynthetic BiologyExpressionBacterialAvailable SinceAug. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPD539 VP16-ZF173
Plasmid#138773PurposeConstitutive expression of VP16-ZF173 under the CMV promoter.DepositorInsertVP16-ZF173
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD526 VP16-ZF13
Plasmid#138755PurposeConstitutive expression of VP16-ZF13 under the CMV promoter.DepositorInsertVP16-ZF13
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJJB1561
Plasmid#218626PurposeExpress geraniol synthase (GES), Idi1, and Erg20(F96W, N127W) all with ePTS1 tags and cytosolic Erg20_WT_synthetic homodimerDepositorInsertGES
TagsePTS1ExpressionYeastAvailable SinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR R4 ATCC LacZ GCTT
Plasmid#173668PurposeDestination vector for Golden Gate Cloning assemblies, blue-white screeningDepositorTypeEmpty backboneExpressionBacterial and InsectAvailable SinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXTK-BB1 [Backbone_RK2-KanR_PartTypeBB]
Plasmid#229387PurposeXanthoMoClo Golden Gate Backbone Plasmid, contains Backbone_RK2-KanR_PartTypeBB and an sfGFP dropoutDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCD-Ugi22
Plasmid#207004PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 22 (Ugi22) sequence (ID2547)DepositorInsertUtricularia gibba insulator 22
UseGolden gate compatible cloning vectorAvailable SinceNov. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCD-Ugi21
Plasmid#207005PurposepGGC000 Greengate entry vector containing Utricularia gibba insulator 21 (Ugi21) sequence (ID9063)DepositorInsertUtricularia gibba insulator 21
UseGolden gate compatible cloning vectorAvailable SinceNov. 8, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD057 VP64-ZF37
Plasmid#138834PurposeConstitutive expression of VP64-ZF37 under the CMV promoter.DepositorInsertVP64-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ132D
Plasmid#69288PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2359 - intron GG2 - 250bp
Plasmid#159878PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG2 - 250bp
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131D
Plasmid#69287PurposeGolden Gate entry vector; 1st gRNA under OsU3 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPD391 VPR-ZF37
Plasmid#138839PurposeConstitutive expression of VPR-ZF37 under the CMV promoter.DepositorInsertVPR-ZF37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEC2735 (pSpy0C2)
Plasmid#220279Purposeintegrative plasmid for heterologous gene expression in S. pyogenes SF370, integrates into the amyA locus via homologous recombinationDepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD159 dsRedExpress2-ZFi43
Plasmid#138799PurposeConstitutive expression of dsRedExpress2-ZFi43 under the CMV promoter.DepositorInsertdsRedExpress2-ZFi43
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ2370 - intron GG3 - 250bp
Plasmid#159879PurposeGolden Gate compatible intron with PATCs for reduced germline silencingDepositorInsertintron GG3 - 250bp
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLOXp-GUS
Plasmid#154059PurposeLevel 0.5 Golden Gate vector, loxP-GUS-nosT-loxPDepositorInsertbeta-glucoronidase (E. coli)
UseSynthetic BiologyExpressionBacterialPromoterN/AAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBlue-TAL
Plasmid#49401PurposeThe pBlue-TAL is suitable for TALEN mutagenesis in insects. It is compatible with Golden Gate Cloning Kit. It has Esp3I cleavage sites and a T7 promoter.DepositorTypeEmpty backboneUseTALENExpressionInsectAvailable SinceDec. 19, 2013AvailabilityAcademic Institutions and Nonprofits only -
pY3
Plasmid#218116PurposeActivity reporter backbone with an empty protease cassette (BsaI) and an empty substrate cassette (BsmBI), both under a divergent pGAL1-10 promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-222
Plasmid#219409PurposeNuclear expression of Synrophin PDZ clamp proteinDepositorInsertpTEF1-NLS-(Syntrophin-PDZ)x4-tADH1
ExpressionYeastAvailable SinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPD535 VP16-ZF129
Plasmid#138768PurposeConstitutive expression of VP16-ZF129 under the CMV promoter.DepositorInsertVP16-ZF129
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD303 VP64-ZF43
Plasmid#138835PurposeConstitutive expression of VP64-ZF43 under the CMV promoter.DepositorInsertVP64-ZF43
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAS1_4x7SKPylT_EF1_Ub-BsmBI_IRES_BSD
Plasmid#162804PurposeSTELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient or stable piggyBac-mediated integrationDepositorInsertUb-BsmBI_IRES_Blasticidin
UsePiggybacExpressionMammalianPromoterEF1alphaAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPD158 dsRedExpress2-ZFi37
Plasmid#138797PurposeConstitutive expression of dsRedExpress2-ZFi37 under the CMV promoter.DepositorInsertdsRedExpress2-ZFi37
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZA31
Plasmid#158509PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
Tags4xMycExpressionPlantMutationL17EAvailable SinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPD385 VPR-ZF43(RAAA)
Plasmid#138824PurposeConstitutive expression of VPR-ZF43(RAAA) under the CMV promoter.DepositorInsertVPR-ZF43(RAAA)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15A, R43A, R71APromoterCMVAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ138A
Plasmid#69280PurposeGolden Gate entry vector; 8th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pYPQ137A
Plasmid#69279PurposeGolden Gate entry vector;7th gRNA under AtU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPD392 VPR-ZF92
Plasmid#138841PurposeConstitutive expression of VPR-ZF92 under the CMV promoter.DepositorInsertVPR-ZF92
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJM450 EF1alpha EBFP2-P2A-BlastR (TUPV1)
Plasmid#139230PurposemMoClo TUPV1, with EF1alpha promoter and EBFP2-P2A-BlastR in the MCSDepositorInsertEBFP2-P2A-Blasticidin S Deaminase
UseSynthetic BiologyExpressionMammalianPromoterEF1alphaAvailable SinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD107 VP16-ZF43(RARR)
Plasmid#138812PurposeConstitutive expression of VP16-ZF43(RARR) under the CMV promoter.DepositorInsertVP16-ZF43(RARR)
UseSynthetic BiologyTags3xFlagExpressionMammalianMutationR15APromoterCMVAvailable SinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD530 VP16-ZF42
Plasmid#138760PurposeConstitutive expression of VP16-ZF42 under the CMV promoter.DepositorInsertVP16-ZF42
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPD531 VP16-ZF54
Plasmid#138762PurposeConstitutive expression of VP16-ZF54 under the CMV promoter.DepositorInsertVP16-ZF54
UseSynthetic BiologyTags3xFlagExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only