We narrowed to 6,202 results for: cas9 expression plasmid

1. pITI1

   Plasmid

   #166094

   Purpose

   Gal inducible expression of dCas9-FokI

   Depositor

   Rebecca Burgess

   Insert

   dCas9-FokI
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9-D10A, H840A

   Promoter

   Gal-inducible

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSC49

   Plasmid

   #104823

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Glyma17g11235 (Dcl4a). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Robert Stupar

   Insert

   Glyma17g11235
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSC38

   Plasmid

   #104812

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from AtUBQ10 promoter.

   Depositor

   Nevin Young

   Insert

   Medtr3g107390
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSC37

   Plasmid

   #104811

   Purpose

   CRISPR/Cas9 2xplex gRNA targeting Medtr3g107390 (MtRdr6). Also expresses Cas9 from Gmubi promoter

   Depositor

   Nevin Young

   Insert

   Medtr3g107390
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-US-ECasE

   Plasmid

   #83961

   Purpose

   Piggyac transposon containing tamoxifen inducible Cas9 and a gRNA cassette.

   Depositor

   Eleanor Chen

   Insert

   ERT2-spCas9-ERT2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   please see depositor comments below

   Promoter

   U6 (gRNA), EF1a (ERT2-Cas9-ERT2)

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti SpBsmBI sgRNA Hygro

   Plasmid

   #62205

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti SpBsmBI sgRNA Puro

   Plasmid

   #62207

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. NM-01

   Plasmid

   #69799

   Purpose

   Bacterial expression of Cas9 with His, MBP, and Strep tags

   Depositor

   Gerard Cagney

   Insert

   Cas9
   

   Use

   Tags

   HIS, MBP, and StrepII

   Expression

   Bacterial

   Mutation

   Aspartate 10 to Alanine (D10A) and Histidine 840 …

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pJB166

   Plasmid

   #86998

   Purpose

   CRISPR/Cas9 in fission yeast using fluoride selection

   Depositor

   Julien Berro

   Article

   Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Feb. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Cetn1/1

    Plasmid

    #50718

    Purpose

    pX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Inserts

    Cetn1 sgRNA1
    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and hU6

    Available since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6(BsmBI)-EF1a-BFP-BSD

    Plasmid

    #117136

    Purpose

    Empty vector encoding BFP-BSD

    Depositor

    Kosuke Yusa

    Insert

    BSD2A
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    modified BFP

    Promoter

    human U6 promoter, human EF1a promoter

    Available since

    April 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCT-tRNA

    Plasmid

    #133813

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pXZ13lacZalphaKanR

    Plasmid

    #160230

    Purpose

    Like pXZ13lacZalpha, but adds second level of selection with Kanamycin resistance.

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCM4.4

    Plasmid

    #217648

    Purpose

    an ermE*p-cas9-driven expression, as an alternative plasmid

    Depositor

    Huimin Zhao

    Article

    Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

    Insert

    Codon Optimized SpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXZ13lacZalpha

    Plasmid

    #160229

    Purpose

    places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

    Depositor

    Ian Duncan

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCJ1023

    Plasmid

    #228763

    Purpose

    Plasmid expressing Cas9 and gRNAs for mouse Ift88 and Pkd2. Use for disruption of mouse Ift88 and Pkd2 in cultured cells.

    Depositor

    Bradley Yoder

    Insert

    Ift88 and Pkd2 gRNAs (Ift88, Pkd2 Mouse)
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    human U6

    Available since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBLO1808_arC9_2xNLS_human

    Plasmid

    #74491

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/2xNLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    Tags

    t2a mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCP-tRNA

    Plasmid

    #133812

    Purpose

    CRISPR-Cas9 system for genetic manipulation of Candida parapsilosis, C. orthopsilosis, and C. metapsilosis

    Depositor

    Geraldine Butler

    Article

    Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

    Insert

    cassette for the expression of the sgRNA from the C. parapsilosis RNA pol II GAPDH promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. VVT1

    Plasmid

    #65779

    Purpose

    The Joung Lab recommends using BPK2660 (Addgene plasmid 70709) instead of VVT1 as guide RNAs cloned into BPK2660 are more effective than this plasmid. Human expression plasmid for S. aureus Cas9 sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    SaCas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCB32

    Plasmid

    #111911

    Purpose

    Expresses gRNA to target Nte1 locus and has HygR marker

    Depositor

    Yi Tang

    Insert

    gRNA cassette targeting Nte1 locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SNR52p

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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