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pBP-BBa_J23100

Plasmid

#74097

Purpose

BBa_J23100 promoter (iGEM collection)

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Bba_J23100 constitutive promoter (Sigma70)

Use

Synthetic Biology

Expression

Bacterial

Promoter

None

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin1

Plasmid

#124852

Purpose

Mutagenesis of Grin1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin1 (Grin1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-RNAPII-CTD wt #14-HA

Plasmid

#160688

Purpose

Expression of GST-RNA Polymerase II C-terminal domain fusion protein. Contains 14 of the consensus heptapeptide repeats shown to be phosphorylated by Cdc2 kinase.

Depositor

Jeffry Corden

Article

Patturajan et al J Biol Chem. 1998 Feb 20;273(8):4689-94.

Insert

RNA Polymerase II CTD

Expression

Bacterial

Mutation

C terminal domain only

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR v2-sgControl

Plasmid

#230080

Purpose

Crispr knock out control

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

non-specific sequence control

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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lenti-NLS-mTagBFP2

Plasmid

#216128

Purpose

Lentiviral vector for expressing mTagBFP2 localizing to the nucleus

Depositor

Stefan Kubicek

Article

Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

Insert

EF1a-NuclearLocalizationSignal-mTagBFP2-WPRE

Use

Lentiviral

Expression

Mammalian

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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Flag pLJM1 RagD wt

Plasmid

#19316

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pFLAG_WT MDA5

Plasmid

#216798

Purpose

Expresses wild type (wt) melanoma differentiation-associated protein 5

Depositor

Sun Hur

Insert

melanoma differentiation-associated protein 5 (IFIH1 Human)

Expression

Mammalian

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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Lenti-dCas9-TurboID-blast

Plasmid

#222599

Purpose

Express fussed dCas9-TurboID for proximity labeling

Depositor

Yarui Diao

Article

Sun et al Nat Genet. 2023 Aug;55(8):1324-1335. doi

Insert

dCas9-TurboID

Use

Lentiviral

Promoter

EF-1α

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_RTN3E-mScarlet3

Plasmid

#216255

Purpose

mammalian expression vector for RTN3E-mScarlet3

Depositor

Edward Avezov

Article

Crapart et al Proc Natl Acad Sci U S A. 2024 Mar 26;12

Insert

reticulon 3 isoform e (RTN3 Human)

Tags

mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pBiFCt-2in1-CC

Plasmid

#105114

Purpose

ratiometric BiFC analysis in plants (transient transformation)

Depositor

Christopher Grefen

Article

Grefen et al Biotechniques. 2012 Oct 2;53(5):311-14.

Type

Empty backbone

Tags

cYFP-Myc/nYFP-HA

Expression

Plant

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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