We narrowed to 2,398 results for: puc19

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qTAG-C-ultraID-Blast

Plasmid

#207715

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pT2ST

Plasmid

#59384

Purpose

Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a trimethoprim resistance gene.

Depositor

Shelley Copley

Article

Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

Insert

TT-ISceI-dfrA

Use

Template

Available Since

Sept. 29, 2014

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Puro-ultraID

Plasmid

#207693

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-dTAG

Plasmid

#207683

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-dTAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-C-mNeon-Blast

Plasmid

#207709

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Puro-mStayGold

Plasmid

#227250

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mStayGold cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-N-Blast-mScarlet

Plasmid

#207678

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mScarlet cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-ultraID

Plasmid

#207682

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pLinker-AID-3xHA-DHFR-LoxP

Plasmid

#86669

Purpose

for generation of AID strains using CRISPR tagging technology

Depositor

David Sibley

Article

Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

Insert

Linker-AID-3xHA-HXGPRT 3'UTR

Use

Expression in toxoplasma gondii

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEF1α-Cre-2A-miRFP670nano3

Plasmid

#207747

Purpose

Expression of the Cre recombinase linked to a miRFP670nano3 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-miRFP670nano3

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mScarlet-Blast

Plasmid

#207711

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-mNeon

Plasmid

#207676

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-mNeon cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-mStayGold-Blast

Plasmid

#227254

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
qTAG-C-3xFLAG-Blast

Plasmid

#207717

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a 3xFLAG-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-C-ultraID-Zeo

Plasmid

#207737

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a ultraID-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Blast-sTagRFP

Plasmid

#207679

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-sTagRFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pEF1α-Cre-2A-mTagBFP2

Plasmid

#207746

Purpose

Expression of the Cre recombinase linked to a mTagBFP2 fluorescent marker

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

Cre-PGK-mTagBFP2

Use

Cre/Lox

Expression

Mammalian

Promoter

EF1alpha

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pAB1620 hU6-BpiI-Cas13bt3-DR

Plasmid

#176315

Purpose

hU6-BpiI-Cas13bt3-DR (crRNA expression)

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2021 Aug 30. pii: 10.103

Insert

Cas13bt3 crRNA direct repeat

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-Zeo

Plasmid

#207733

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
qTAG-N-Zeo-ultraID

Plasmid

#207704

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-ultraID cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge