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zPRL3_1-169_C104D_pET-15b

Plasmid

#250560

Purpose

Bacterial expression of zebrafish PRL3/PTP4A3 C104D

Depositor

Kalle Gehring

Insert

zebrafish PRL3 (ptp4a3a Zebrafish)

Tags

His-tag

Expression

Bacterial

Mutation

Cysteine 104 to Aspartic acid (C104D)

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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zPRL2_1-174_pET-15b

Plasmid

#250559

Purpose

Bacterial expression of zebrafish PRL2/PTP4A2

Depositor

Kalle Gehring

Insert

zebrafish PRL2 (ptp4a2b Zebrafish)

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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zPRL1_1-173_pET-15b

Plasmid

#250558

Purpose

Bacterial expression of zebrafish PRL1/PTP4A1

Depositor

Kalle Gehring

Insert

zebrafish PRL1 (ptp4a1, prl Zebrafish)

Tags

His-tag

Expression

Bacterial

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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hsp70I: cPla-mKate2-P2A-EGFP-KDEL

Plasmid

#250915

Purpose

Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in zebrafish live tissue after heat shock

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

cPla2-mKate2-P2A-EGFP-KDEL (pla2g4aa Zebrafish)

Use

D.rerio (zebrafish)

Tags

KDEL on EGFP and mKate2 on cPla2

Promoter

hsp70i

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro ArfGAP1_ALPS1_ALPS2-mKate2-P2A-EGFP-LMNB1

Plasmid

#250905

Purpose

Dual color expression of mKate2 tagged human ARFGAP1 ALPS 1-2 and EGFP tagged zebrafish LaminB1 in mammallian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-P2A-EGFP-Lamin B1 (lmnb1, ARFGAP1 Human, Zebrafish)

Tags

EGFP on LMNB1 and mKate2 on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 192–304 only

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pSB-CMV-MCS-Puro-ArfGAP1_ALPS1-mKate2-3NLS-P2A-eGFP-LMNB1

Plasmid

#250914

Purpose

Dual color expression of mKate2-3xNLS tagged rat ALPS1 and eGFP tagged zebrafish Lamin B1 in mammalian cell lines

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

ArfGAP1-mKate2-3xNLS-P2A-EGFP-LMNB1 (lmnb1, Arfgap1 Zebrafish, Rat)

Tags

EGFP on LMNB1 and mKate2 on ArfGAP1

Expression

Mammalian

Mutation

ARFGAP1 amino acids 196–234 only

Promoter

CMV

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2+Brd9 (zebrafish) partial UTR and coding sequence-EGFP

Plasmid

#248787

Purpose

fuse partial UTR and coding sequence of Brd9 (zebrafish) with EGFP reporter

Depositor

Wenyan Ren

Article

Wu et al Commun Biol. 2025 Dec 1. doi: 10.1038/s4

Insert

brd9 (brd9 Zebrafish)

Use

Brd9(zebrafish)-egfp reporter

Tags

EGFP

Promoter

partial 5'UTR sequence of zebrafish brd9

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_drePum1_HA_dreAdar1

Plasmid

#250541

Purpose

In vitro transcription (SP6 promoter) or mammalian expression (CMV promoter) of zebrafish Pum1 fused to the catalytic domain of zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

drePum1_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_dreAdar1_E488Q

Plasmid

#250533

Purpose

In vitro transcription (SP6 promotor) or mammalian expression (CMV promotor) of the catalytic domain of zerbafish Adar1 (E488Q)

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_dreAdar1_E488Q

Use

Synthetic Biology

Mutation

E488Q

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pCS2_N22_HA_dreAdar1

Plasmid

#250532

Purpose

In vitro transcription (SP6 promoter) or mammalian expression (CMV promoter) of N22 peptide fused to the catalytic domain on zebrafish Adar1

Depositor

Daniel Cifuentes

Article

Kretov et al Mol Cell. 2026 Feb 6:S1097-2765(26)00023

Insert

N22_HA_dreAdar1

Use

Synthetic Biology

Available Since

Jan. 15, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mTFP1-Rab5c

Plasmid

#244031

Purpose

fusion of Rab5c to mTFP1

Depositor

Markus Affolter

Article

Vigano et al Biol Open. 2018 Oct 29;7(11). pii: bio.0

Insert

mTFP1-Rab5c

Expression

Mammalian

Available Since

Oct. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-QUAS:cPla2-mKate2

Plasmid

#241913

Purpose

Zebrafish transgenesis plasmid encoding D. rerio cPla2-mKate2, driven by the QUAS enhancer, and including a cmlc2:EGFP cardiac transgenesis marker

Depositor

Balázs Enyedi

Article

Fazekas et al J Cell Biol. 2026 Jan 5;225(1):e20250116

Insert

QUAS enhancer driving cPla2-mKate2

Use

Zebrafish tol2 transgenesis

Tags

cPla2 fused to mKate2

Promoter

QUAS

Available Since

Oct. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+her3WT-P2A-sfGFP

Plasmid

#240545

Purpose

Wild-type zebrafish Her3 (ortholog to human HES3) in a -2A-sfGFP backbone for visualization

Depositor

Genevieve Kendall

Article

Kent et al Dev Biol. 2023 Apr;496:1-14. doi: 10.101

Insert

her3 (her3 Zebrafish)

Expression

Mammalian

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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apoBb.1-Dendra2

Plasmid

#218180

Purpose

used for TALEN HDR to generate endogenous fusion of HA-Dendra2 to apoBb.1 in zebrafish

Depositor

Steven Farber

Article

Moll et al Arterioscler Thromb Vasc Biol. 2025 Aug

Insert

apoBb.1-Dendra2

Use

TALEN

Tags

fuses HA-Dendra2 to apoBb.1

Mutation

inserts HA-Dendra2 sequence at C-terminus of apoB…

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ Zf nhsl1b-mNeongreen

Plasmid

#233883

Purpose

mNeongreen tagged form of zebrafish nhsl1b. For in vitro transcription (SP6) or expression through CMV.

Depositor

Nicolas David

Article

Escot et al Commun Biol. 2025 Feb 28;8(1):328. doi:

Insert

nhsl1b (nhsl1b Zebrafish)

Use

In vitro synthesis of mrna

Tags

mNeongreen

Expression

Mammalian

Available Since

July 18, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG-KS

Plasmid

#238298

Purpose

For RNA injection of Nanog-mNG-KS into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG-KS

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG-KS-FtoG

Plasmid

#238297

Purpose

For RNA injection of Nanog-mNG-KS-FtoG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG-KS-FtoG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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Nanog-mNG

Plasmid

#238296

Purpose

For RNA injection of Nanog-mNG into Zebrafish

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

Nanog-mNG

Use

Zebrafish expression

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pTol1-Spe-KpnI-ubb-nmKate

Plasmid

#238373

Purpose

Empty backbone to generate guideRNA shuttles, that also drives ubiquitous expression of nuclear mKate.

Depositor

Juan Manuel González-Rosa

Article

Keeley et al Cell Rep Methods. 2025 Mar 24;5(3):10100

Insert

nmKate

Promoter

ubiquitin

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ dgat1b-FLAG

Plasmid

#232144

Purpose

Expresses zebrafish dgat1b with a C-terminal FLAG tag under control of the CMV promoter

Depositor

Steven Farber

Article

Wilson et al J Biol Chem. 2024 Nov 6;300(12):107973.

Insert

dgat1b

Use

Zebrafish expression

Tags

FLAG

Promoter

CMV

Available Since

April 25, 2025

Availability

Academic Institutions and Nonprofits only

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