Addgene

We narrowed to 5,867 results for: pcDNA 3.1

Showing: 1891 - 1920 of 5867 results

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pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

Plasmid

#114112

Purpose

fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

NanoLuc; Volvox channelrhodopsin 1; EYFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-OTUB1 T134R,C91S-pcDNA3.1

Plasmid

#118212

Purpose

Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

Depositor

Cynthia Wolberger

Article

Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

Insert

OTUB1 (OTUB1 Human)

Use

Tags

3x FLAG

Expression

Mammalian

Mutation

changed cysteine 91 to serine and threonine 134 t…

Promoter

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

Plasmid

#64207

Purpose

mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

Depositor

Narasimhan Gautam

Article

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Inserts

Cry2 (CRY2 Mustard Weed)
RGS4(del 1-33) (RGS4 Human)

Use

Tags

mcherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
Kv7.4 (1-645, Δ368-523)/pcDNA3.1

Plasmid

#111456

Purpose

Electrophysiology in HEK cell

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)

Use

Tags

Expression

Mammalian

Mutation

Kv7.4 (1-645, Δ368-523), C643A

Promoter

Available since

May 15, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 CMV NAPA-N BGH

Plasmid

#92278

Purpose

FRET probe; extracellular synapse targeted mCherry

Depositor

Baljit Khakh

Article

Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

Insert

Neuron-astrocyte proximity assay - Neuronal

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER

Plasmid

#16753

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3-deltaCTER (DVL3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

delta CTER

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 (+) Tac-delta scr

Plasmid

#80019

Purpose

Mammalian expression of Tac-delta scr

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tac (IL2RA Human)

Use

Tags

KLRFGFK

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLRFGFK

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1/NDUFS2 _34-78/myc-His

Plasmid

#127496

Purpose

Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-78 (NDUFS2 Human)

Use

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-78

Promoter

CMV

Available since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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Human NCKX4-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75212

Purpose

Mammalian expression of SLC24A4

Depositor

Jonathan Lytton

Article

Li et al J Biol Chem. 2002 Dec 13;277(50):48410-7

Insert

SLC24A4 (SLC24A4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-577-CFP-578CT

Plasmid

#41682

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Use

Tags

CFP

Expression

Mammalian

Mutation

“Monomerizing” CFP A206K mutation; CFP inserted d…

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)mGAT1-577-YFP-578CT

Plasmid

#41681

Depositor

Henry Lester

Article

Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

Insert

Mus musculus GABA transporter 1 (Slc6a1 Synthetic, Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

“Monomerizing” YFP A206K mutation; YFP inserted d…

Promoter

CMV

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-short-FlagTag (pcDNA3.1+)

Plasmid

#75202

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLAG-SBP-STAG2 (S1215X)

Plasmid

#73970

Purpose

STAG2 cDNA (CCDS43990) S1215X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1215X

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (Q1117X)

Plasmid

#73968

Purpose

STAG2 cDNA (CCDS43990) Q1117X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

Q1117X

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S1075X)

Plasmid

#73967

Purpose

STAG2 cDNA (CCDS43990) S1075X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S1075X

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-FLAG-SBP-STAG2 (S653X)

Plasmid

#73965

Purpose

STAG2 cDNA (CCDS43990) S653X

Depositor

Todd Waldman

Article

Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

Insert

STAG2 (STAG2 Human)

Use

Tags

FLAG-SBP

Expression

Mammalian

Mutation

S653X

Promoter

Available since

March 7, 2016

Availability

Academic Institutions and Nonprofits only
pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

Plasmid

#238007

Purpose

300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.

Depositor

Simon Powell, Agnel Sfeir

Article

Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:

Insert

AAVS1 (AAVS1, PPP1R12C Human)

Use

Rna donor to study rt-dsbr

Tags

Expression

Mammalian

Mutation

3bp insertion signature (ATC) at Cas9 cut site in…

Promoter

CMV

Available since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-lk-HiBiT

Plasmid

#233064

Purpose

Expresses mouse ADGRL3 (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide. Also includes a C-terminal HiBiT tag.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

ALFA and HiBiT

Expression

Mammalian

Mutation

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-T923G

Plasmid

#233063

Purpose

Expresses cleavage-deficient mouse ADGRL3-T923G (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.

Depositor

Jonathan Javitch

Article

Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.

Insert

ADGRL3 (Adgrl3 Mouse)

Use

Tags

ALFA and HiBiT

Expression

Mammalian

Mutation

Threonine 923 to Glycine

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Cas9-P2A-dTomato-PS9

Plasmid

#231911

Purpose

For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'PS9' packaging signal

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

Cas9-P2A-dTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Cas9-P2A-dTomato-T20

Plasmid

#231912

Purpose

For packaging spCas9, dTomato mRNA into SARS-CoV-2 virus-like particles (VLPs) with a 'T20' packaging signal

Depositor

Sriram Neelamegham

Article

Yang et al Nucleic Acids Res. 2025 Feb 27;53(5):gka

Insert

Cas9-P2A-dTomato

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 T21R-Nb139-T2A-mCherry

Plasmid

#225589

Purpose

Mammalian expression vector for TRIM21 RING-Nb139 anti-p53 degrader with a self-cleaving T2A-mCherry fluorescent expression reporter

Depositor

William McEwan

Article

Benn et al Science. 2024 Aug 30;385(6712):1009-1016

Insert

T21R-Nb139-T2A-mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc:Skp1(dC111):gs10:FRB:HA

Plasmid

#228532

Purpose

A fragment of human Skp1 protein in fusion with FRB domain through a flexible glycine-serine linker.

Depositor

Roman Jerala

Article

Verbic et al ACS Synth Biol. 2024 Mar 15;13(3):792-80

Insert

myc:Skp1(dC111):gs10:FRB:HA (SKP1 Synthetic, Human)

Use

Tags

HA and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-GTSE1-S91, 262 ,724A

Plasmid

#224737

Purpose

For expression of HA-tagged GTSE1 protein with S91, 262 ,724A mutation

Depositor

Michele Pagano

Article

García-Vázquez et al bioRxiv 2024.06.26.600797

Insert

GTSE1 (GTSE1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 91, 262 ,724 to Alanine

Promoter

CMV

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46-P2A-Electra1 (pAH9)

Plasmid

#227614

Purpose

Overexpression of SLC25A46 in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

Plasmid

#227619

Purpose

Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

deleted aa 2-83

Promoter

CMV, T7

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

Plasmid

#222861

Purpose

This plasmid codes for the OgeuIscB nickase

Depositor

Vincent Dion

Article

Mangin et al bioRxiv 2023.10.24.563270

Insert

OgeuIscB Nickase

Use

CRISPR

Tags

3X HA and Nucleoplasmin NLS and ITR2

Expression

Mammalian

Mutation

E193A for nickase mutation

Promoter

CMV

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM1-WT

Plasmid

#215898

Purpose

Expression of TNRC6A-CIM1 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1224-1528

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-myc-GFP-TNRC6A-SD-CIM2-WT

Plasmid

#215899

Purpose

Expression of TNRC6A-CIM2 domain

Depositor

Kumiko Ui-Tei

Article

Munakata et al Genes (Basel). 2021 Feb 13;12(2):271. do

Insert

TNRC6A (TNRC6A Human)

Use

Tags

myc-GFP

Expression

Mammalian

Mutation

contain aa1529-1709

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EGFP-Rac1(G12C, K96H)

Plasmid

#224281

Purpose

Cell transfection and expression of Rac1 (G12C, K96H)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rac1 (RAC1 Human)

Use

Tags

Expression

Mammalian

Mutation

G12C, K96H

Promoter

Available since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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