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pcDNA 3.1(-) mouse Egr2

Plasmid

#107997

Purpose

Expresses mouse Egr2 in mammalian cells

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr2 (Egr2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::rtTA3-bGHpA

Plasmid

#62445

Purpose

MXS_chaining vector with CMV::rtTA3-bGHpA

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with CMV promoter

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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CENP-B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45214

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

CENP-B targeted Aurora B FRET sensor

Expression

Mammalian

Promoter

t7

Available Since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pYTK-DN1

Plasmid

#180282

Purpose

Cas9 cassette (TU1)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS - pPGK1 - Cas9 - tPGK1 - ConR1

Expression

Bacterial

Promoter

pPGK1

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCI-myc-USP10

Plasmid

#184034

Purpose

Expresses myc-USP10 construct in mammalian cells

Depositor

Juan Bonifacino

Article

Jia et al J Biol Chem. 2021 Jan-Jun;296:100405. do

Insert

USP10 (USP10 Human)

Tags

myc

Expression

Mammalian

Promoter

CMVf

Available Since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pAd5-B6/7 ∆E3-GFP

Plasmid

#179202

Purpose

A block for the AdenoBuilder genome assembly system. The insert consolidates the regions present in pAd5-B6∆E3-GFP and pAd5-B7.

Depositor

Fred Bunz

Article

Jang et al STAR Protoc. 2022 Jan 20;3(1):101123. do

Insert

Modified block 6/7, with deletion in E3 and insertion of GFP cassette

Use

Adenoviral

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-tdTomato-polyA

Plasmid

#174022

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

tdTomato

Use

Zebrafish knock-in tagging

Available Since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-p2A-eGFP-MCS-polyA

Plasmid

#174028

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Article

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

p2A-eGFP

Use

Zebrafish knock-in tagging

Available Since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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NHA-hnRNPK

Plasmid

#120389

Purpose

Expression of HA tagged hnRNPK-lambdaN fusion

Depositor

Igor Ulitsky

Article

Lubelsky et al Nature. 2018 Mar 1;555(7694):107-111. do

Insert

hnRNPK (HNRNPK Human)

Tags

HA and LambdaN

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2019

Availability

Academic Institutions and Nonprofits only

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nuc-gTEMP_pcDNA3

Plasmid

#109118

Purpose

Nucleus targetting gTEMP

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

nuc-gTEMP

Expression

Mammalian

Promoter

CMV

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)-LZ-CD95L

Plasmid

#104349

Purpose

Expresses human CD95L leucine zipper in mammalian cells

Depositor

Alexander McLellan

Article

Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

Insert

CD95L leucine zipper (FASLG Human)

Tags

octahistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSR78

Plasmid

#90210

Purpose

Expresses the light sensors CcaS and Cph8 as well as the phycocyanobilin biosynthesis genes Ho1 and PcyA

Depositor

Jeffrey Tabor

Article

Olson et al Mol Syst Biol. 2017 Apr 24;13(4):926. do

Inserts

CcaS
Cph8
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pSMP-EED_2

Plasmid

#36386

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

EED (EED Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSMP-SETDB1_2

Plasmid

#36348

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

SETDB1 (SETDB1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-Bmi1_3

Plasmid

#36354

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Bmi1 (BMI1 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pSMP-G9A_2

Plasmid

#36332

Depositor

George Daley

Article

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

G9A (EHMT2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only
pLD-puro-MTS-EGFP

Plasmid

#190270

Purpose

Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

Depositor

Christian Münch

Article

Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

Insert

MTS-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki67-mCherry

Plasmid

#183748

Purpose

Expresses EGFP-Ki67-mCherry

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Tags

Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-eGFP-MinD

Plasmid

#133622

Purpose

Expression vector for the Escherichia coli MinD with an N-terminal fusion of a His-tag for purification and an EGFP for visualization.

Depositor

Petra Schwille

Article

Zieske et al FEBS Lett. 2014 Aug 1;588(15):2545-9. do

Insert

EGFP-MinD (minD E. coli)

Tags

His

Expression

Bacterial

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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Sirius-mT-Sapphire_pcDNA3

Plasmid

#89584

Purpose

Genetically encoded ratiometric fluorescent thermometer

Depositor

Takeharu Nagai

Article

Nakano et al PLoS One. 2017 Feb 17;12(2):e0172344. do

Insert

Sirius-mT-Sapphire

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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