We narrowed to 10,942 results for: NSI;

1. pCDNA3.1 AT1AR-324delta

   Plasmid

   #45635

   Depositor

   Robert Lefkowitz

   Article

   Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

   Insert

   AT1AR-324delta (Agtr1a Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   added stop codon at 324 aa

   Promoter

   CMV

   Available since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pUE1-TSP-ORF68 K395A/K396A

   Plasmid

   #162656

   Purpose

   Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acids K395 and K396 mutated to alanine

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. As3 (pBW411J117-arsR)

   Plasmid

   #123142

   Purpose

   An arsenic sensor with optimized intracellular receptor density. Also used in the sensor cell array. pSB3K3 carrying J117-30arsR-t-ParsR-30gfp-t

   Depositor

   Baojun Wang

   Article

   Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

   Insert

   J117-30arsR-t-ParsR-30gfp-t
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J117, ParsR

   Available since

   May 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p11_AaC60αβ/C20

   Plasmid

   #229506

   Purpose

   Expresses Anthoceros agrestis Chaperonin 60α, Chaperonin 60β, Chaperonin 20

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Chaperonin 60α
   Chaperonin 60β
   Chaperonin 20
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCfB2188-PrARG8-GFP::URA3

   Plasmid

   #216512

   Purpose

   Integrative fluorescent reporter for amino acid biosynthesis sensing pathways, comprising GFP driven by the ARG8 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrARG8-yeGFP
   

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ARG8

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCfB2197-PrGNP1-mK2-NatMX

   Plasmid

   #216513

   Purpose

   Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrGnp1-mKate2
   

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GNP1

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. Vinculin-Clover-CC-S7-mRuby2

   Plasmid

   #233236

   Purpose

   Vinculin FRET tension sensor with coiled-coil S7 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S7-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Vinculin-Clover-CC-S4-mRuby2

   Plasmid

   #233235

   Purpose

   Vinculin FRET tension sensor with coiled-coil S4 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S4-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Vinculin-Clover-CC-S2-mRuby2

   Plasmid

   #233234

   Purpose

   Vinculin FRET tension sensor with coiled-coil S2 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S2-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pIVT-Bxb1-I87L, A369P, E434G

    Plasmid

    #232750

    Purpose

    IVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Expression

    Mutation

    I87L, A369P, E434G

    Promoter

    Available since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXJ-Myc-KLF16

    Plasmid

    #232655

    Purpose

    transient expresses KLF16 in mammalian cells

    Depositor

    Chen-Yang Shen

    Article

    Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

    Insert

    KLF16 (KLF16 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1-GT_CAG-ITPKA-mScarlet-I

    Plasmid

    #229778

    Purpose

    Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mScarlet-I
    

    Use

    Synthetic Biology

    Tags

    ITPKA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. Vinculin-Clover-CC-Mad-mRuby2

    Plasmid

    #233239

    Purpose

    Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vinculin-Clover-CC-Mad-mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Vinculin-Clover-CC-S0-mRuby2

    Plasmid

    #233233

    Purpose

    Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vinculin-Clover-CC-S0-mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

    Plasmid

    #229790

    Purpose

    Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

    Plasmid

    #229800

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern; mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-22b(+) pCopA RBS30 sfgfp

    Plasmid

    #226371

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    sfGFP
    

    Use

    Tags

    6x HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    copA promoter (pCopA) and RBS30 regulation

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. hnRNPAB X2-IDR

    Plasmid

    #216517

    Purpose

    Expresses MBP-tagged intrinsically disordered region of hnRNPAB X2

    Depositor

    Nicolas Fawzi

    Article

    O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

    Insert

    hnRNPAB X2-IDR
    

    Use

    Tags

    6xHis tags-MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 2-225

    Promoter

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac SOX 318-320A

    Plasmid

    #192060

    Purpose

    Expression vector for SOX (ORF37) 318-320A

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (ORF37)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    318-320A

    Promoter

    Polyhedrin

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SOX D221A/E244A/K246A pACEmam1

    Plasmid

    #192050

    Purpose

    Expresses a 3X catalytic mutant of SOX (ORF37)

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (3X catalytic mutant)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    3X catalytic mutant D221A/E244A/K246A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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