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pHR RPS27A K107/113R-3xFlag_IRES-GFP

Plasmid

#198392

Purpose

RPS27A K107/113R (ubiquitin/ribosomal protein eS31 fusion) expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

RPS27A (RPS27A Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

ubiquitination site double mutant (K107/113R)

Promoter

EF1A

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrobin

Plasmid

#136829

Purpose

Mammalian expression of the centrosomal protein Centrombin N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrobin (CNTROB Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV CAG ChR2 E123T T159C 2A tDimer

Plasmid

#85399

Purpose

High efficiency channelrhodopsin with cytomegalovirus enhancer fused to chicken β-actin (CAG) promoter

Depositor

Thomas Oertner

Article

Berndt et al Proc Natl Acad Sci U S A. 2011 May 3;108

Inserts

Channelrhodopsin-2
red fluorescent protein

Use

AAV

Tags

Expression

Mammalian

Mutation

E123T , T159C

Promoter

CAG and ribosomal skip sequence 2A

Available since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcsII-pb6-flag

Plasmid

#86770

Purpose

C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

Depositor

Do-Hyung Kim

Article

Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

Insert

proteasome beta subunit 6 (PSMB6 Human)

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

Promoter

unknown

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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pBiex PKC - Peptide 8 mCit mCer

Plasmid

#83563

Purpose

Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PRKCA (PRKCA Human)

Use

Tags

10 nm ER/K Helix, FLAG purification tag, TEV Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pCXLE-hK + E2-Crimson

Plasmid

#74946

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Klf4 (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-Cep57

Plasmid

#136817

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep57 (CEP57 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-Cep57

Plasmid

#136867

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep57 (CEP57 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep72

Plasmid

#136819

Purpose

Mammalian expression of the centrosomal protein Cep72 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep72 (CEP72 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Plasmid

#171616

Purpose

Soma-targeted Cal-Light with Kv2.1

Depositor

Hyungbae Kwon

Article

Hyun et al Nat Commun. 2022 Dec 12;13(1):7692. doi:

Insert

TM-Kv2.1-CaM-NES-TevN-AsLOV2-TEVseq-tTA

Use

Synthetic Biology

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pEBTetDBl-CLIP-CP110

Plasmid

#136852

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-CP110 (CCP110 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-Kv2.1C-TlcnC

Plasmid

#114376

Purpose

expresses Flpo recombinase-dependent GtACR2-Kv2.1C-TlcnC, which targets GtACR2 to the somatodendritic compartment

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP-Kv2.1C-TlcnC (NEWENTRY )

Use

AAV

Tags

EYFP, Kv2.1C, and TlcnC

Expression

Mammalian

Mutation

Promoter

Ef1a

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-CP110

Plasmid

#136851

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-CP110 (CCP110 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetBl-CLIP-Centrobin

Plasmid

#136878

Purpose

Mammalian expression of the centrosomal protein Centrombin N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Centrobin (CNTROB Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetD-SNAP-Centrin2

Plasmid

#136785

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep164

Plasmid

#136824

Purpose

Mammalian expression of the centrosomal protein Cep164 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep164 (CEP164 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTet-SNAP-KIAA0841

Plasmid

#136810

Purpose

Mammalian expression of the centrosomal protein KIAA0841 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-KIAA0841 (HAUS5 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Centrin2

Plasmid

#136784

Purpose

Mammalian expression of the centrosomal protein Centrin2 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Centrin2 (CETN2 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetBl-CLIP-Cep57

Plasmid

#136866

Purpose

Mammalian expression of the centrosomal protein Cep57 N-terminally fused to CLIP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

CLIP-Cep57 (CEP57 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 31, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEBTetD-SNAP-CP110

Plasmid

#136803

Purpose

Mammalian expression of the centrosomal protein Cep110 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-CP110 (CCP110 Human, Synthetic)

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge