We narrowed to 11,217 results for: Vars;
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Plasmid#159725PurposeExpress MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
UseTagsGal4DBD and MYITV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available SinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX-FHH-MAPKAP1RBD
Plasmid#120710PurposeExpresses Flag-HA-6xHIS-MAPKAP1 RBD fusion protein in mammalian cells & for virus productionDepositorInsertMAPKAP1 RBD
UseLentiviralTagsFlag-HA-6xHISExpressionMammalianMutationAmino acids 279-353PromoterCMVAvailable SinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW106
Plasmid#111138PurposeFor the expression of rat AANAT with Met-Ser-Ile N-terminus in S. cerevisiaeDepositorInsertMSI-ratAANAT-3FLAG (Leu2 deletion) (Aanat Rat)
UseTags3x FlagExpressionYeastMutationLeu2 deletionPromoterGal1Available SinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSETa-Amrose v2
Plasmid#79538PurposepRSETa (Invitrogen) plasmid expressing Amrose variant 2DepositorInsertAmrose v2
UseTags6xHis, T7 epitope, and Xpress tagExpressionBacterialMutationPromoterT7Available SinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
PMKO-sh_lnc-BATE1
Plasmid#64866PurposeExpresses shRNA targeting lnc-BATE1DepositorInsertshRNA targeting lnc-BATE1
UseRetroviralTagsExpressionMutationPromoteru6Available SinceMay 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB786
Plasmid#60151PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by LEU1 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationFLuc has A4V & S504G (no functional effect)PromoterLEU1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB693
Plasmid#60145PurposeDisintegrator plasmid encoding MET17 promoter driven yellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable SinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB812
Plasmid#60160PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by RNR1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterRNR1prAvailable SinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCell-Free_KA2_ZIKV NS2B
Plasmid#145364PurposeCell-free/mammalian expression vector for Zika virus NS2B protein with N-term mCherryDepositorInsertZIKV NS2B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable SinceAvailabilityAcademic Institutions and Nonprofits only -
pDL025_HA-rb_IgG_HC_15F11
Plasmid#193622PurposeHA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.DepositorInsertAnti_HA_15F11 rabbit heavy chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDL026_HA-rb_IgG_LC_15F11
Plasmid#193623PurposeHA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.DepositorInsertAnti-HA_15F11 rabbit light chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable SinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
MAC_N_KLF9
Plasmid#167774PurposeExpresses KLF9 in mammalian cellsDepositorAvailable SinceJan. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG
Plasmid#138566PurposeExpresses human codon-optimized rationally engineered SpCas9 variant and blasticidin resistance: EFS promoter-SpCas9-NG-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationL1111R, D1135V, G1218R, E1219F, A1322R, R1335V, T…PromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
xCas9
Plasmid#138565PurposeExpresses human codon-optimized expanded PAM SpCas9 variant and blasticidin resistance: EFS promoter-xCas9-NLS-FLAG-P2A-BSDDepositorInsertxCas9
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterEFSAvailable SinceJune 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBR322-TIMER
Plasmid#103056Purposemedium copy plasmid for expression of a variant of the fluorescent timer protein suitable for bacteria under the control of the constitutive ybaJp promoter (works in E. coli and Salmonella enterica)DepositorInsertTIMER-bac
UseTagsExpressionBacterialMutationmutations: S4T and S179TPromoterconstitutive ybaJp promoterAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CRK_SH3
Plasmid#178472PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertCRK_SH3 (CRK Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available SinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLentiGuide
Plasmid#117986PurposepLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.DepositorInsertsgRNA backbone (F+E)
UseLentiviralTagsExpressionMutationPromoterAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PH-Btk-GFP
Plasmid#51463PurposeIs a biosensor for PIP3DepositorAvailable SinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)- e18
Plasmid#131670PurposeExpresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.DepositorInserte18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMVAvailable SinceSept. 26, 2019AvailabilityAcademic Institutions and Nonprofits only