We narrowed to 165,347 results for: addgene

1. pMSCV-Flag-Mm Ezh2 Y641F

   Plasmid

   #80077

   Purpose

   Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection

   Depositor

   Phillip Abbosh

   Inserts

   Flag-EZH2 Y641F (Ezh2 Mouse)
   Hygromycin phosphotransferase
   

   Use

   Retroviral

   Tags

   5' FLAG

   Expression

   Mammalian

   Mutation

   Y641F, R342R, N393N

   Available Since

   July 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. CFL1 (CFL1A-c002)

   Plasmid

   #98237

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   CFL1A (CFL1 Human)
   

   Tags

   His-6-TEV

   Expression

   Bacterial

   Mutation

   pfam00241:Cofilin_ADF 27-148

   Promoter

   T7

   Available Since

   Sept. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB_EF1a_FLAG-tNGFR_ZeoR

   Plasmid

   #212649

   Purpose

   piggyBac vector to express FLAG-tNGFR with zeocin resistance

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   tNGFR
   

   Use

   Piggybac

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

   Plasmid

   #201151

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Inserts

   SadCas9-KRAB
   gRNA-TRE
   

   Use

   CRISPR and Lentiviral

   Tags

   HA

   Mutation

   gRNA sequence: ATCAGTGATAGAGAACGTATG

   Available Since

   July 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCLXSN-GFP

   Plasmid

   #27557

   Depositor

   Shao-Cong Sun

   Article

   Reiley et al Mol Cell Biol. 2005 May . 25(10):3886-95

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Aug. 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. KLHL3 (KLHL3A-c003)

   Plasmid

   #110251

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   KLHL3 (KLHL3 Human)
   

   Tags

   His-TEV

   Expression

   Bacterial

   Promoter

   T7 - lacO

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEZYshuttle

   Plasmid

   #18703

   Purpose

   pShuttle-CMV converted Gateway Destination vector for AdEasy adenoviral vector system

   Depositor

   Yu-Zhu Zhang

   Article

   Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

   Type

   Empty backbone

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 26, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG306-GPD-ccdB chr I

   Plasmid

   #41894

   Depositor

   Dan Gottschling

   Article

   Hughes et al Nature. 2012 Dec 13;492(7428):261-5. doi

   Type

   Empty backbone

   Expression

   Yeast

   Promoter

   GPD

   Available Since

   Feb. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. 5VC9 (CXXC4)

   Plasmid

   #101263

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CXXC4 (CXXC4 Human)
   

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. KEAP1 (KEAP1A-c029)

    Plasmid

    #110247

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KEAP1 (KEAP1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

    Plasmid

    #159912

    Purpose

    Mutagenesis of Kcnd3

    Depositor

    Larry Zweifel

    Insert

    Kcnd3 (Kcnd3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. tol2-4xnr UAS-PA Rac1-mCherry

    Plasmid

    #41878

    Depositor

    Anna Huttenlocher

    Insert

    photoactivatable Rac1 (RAC1 Human)
    

    Use

    Zebrafish expression

    Tags

    6x His and mCherry

    Mutation

    Rac1 starts at I4 and contains mutations Q61L, E9…

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. 5WCI (MYST1)

    Plasmid

    #101269

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    MYST1 (KAT8 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 2, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. ANO10 (TMEM16KA-c001)

    Plasmid

    #110267

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    TMEM16K (ANO10 Human)
    

    Tags

    TEV-His10-Flag

    Expression

    Insect

    Promoter

    polyhedrin promoter

    Available Since

    April 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_sfGFP150K

    Plasmid

    #193313

    Purpose

    pAS control plasmid for sfGFP expression

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Methods Mol Biol. 2023;2676:169-180. doi

    Insert

    super folder GFP
    

    Expression

    Mammalian

    Mutation

    150K in sfGFP

    Promoter

    EF1

    Available Since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 5TTW (EED)

    Plasmid

    #101254

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    EED (EED Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 30, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. CBX7

    Plasmid

    #25241

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CBX7_04 (CBX7 Human)
    

    Tags

    6x His and TEV cleavage site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 13, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

18. ChR2-YFP-NavII-III

    Plasmid

    #26057

    Depositor

    Matthew Grubb

    Article

    Grubb et al PLoS One. 2010 . 5(10):e13761.

    Insert

    NavII-III
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Available Since

    Nov. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

19. 6BYB (L3MBTL1)

    Plasmid

    #119957

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    L3MBTL1 (L3MBTL1 Human)
    

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pATF4.1.0-gDNA

    Plasmid

    #113772

    Purpose

    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ATF4

    Depositor

    Kevin White

    Insert

    ATF4 (ATF4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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