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We narrowed to 15,857 results for: cas9

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Showing: 1261 - 1280 of 15857 results

  3. pGL3- sgAAVS1.5-CAG-Cas9-T2A-mCherry-P2A-Puro

    Plasmid
    #216246
    Purpose
    Express Cas9 with CAG promoter to improve the expression in hES cells and a highly efficient sgAAVS1.5 for AAVS1 integration
    Depositor
    Elina Ikonen
    Publication
    Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9 and sgAAVS1.5
    Use
    CRISPR; Human safe harbor locus (a avs1) site-spe…
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    216246-plasmid-map-sequence-id-426842

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  5. pX330-U6-(sgRNA-T2)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223323
    Purpose
    Human codon optimized Cas9 expressing plasmid that carries the sgRNA T2 from Mali et al., 2013 (10.1126/science.1232033).
    Depositor
    Jonathan Arias
    Publication
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Insert
    SpCas9 and sgRNA-T2 (cas9 )
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    223323-plasmid-map-sequence-id-447744

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  6. pISV-CRISPR/Cas9PGmUbi

    Plasmid
    #209189
    Purpose
    Expression of Cas9 in the model legume Medicago truncatula, Gmubi promoter (PGmubi), DsRed and Basta selection
    Depositor
    Christian Staehelin
    Inserts
    Cas9
    DsRed(deltaB)
    Tags
    NLS
    Expression
    Plant
    Mutation
    missing BsaI site and plant-codon optimized with …
    Promoter
    CaMV 35S and GmUbi from Glycine max (L.) Merr. cv…
    Available since
    Jan. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    209189-plasmid-map-sequence-id-419145

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  8. pCMV-PE7-P2A-GFP

    Plasmid
    #222996
    Purpose
    Mammalian expression of SpCas9 PE7 prime editor with P2A-EGFP marker
    Depositor
    Brittany Adamson
    Publication
    Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PE7-P2A-EGFP
    Tags
    P2A-EGFP, SV40 bpNLS, and c-Myc NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    222996-plasmid-map-sequence-id-442553

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  9. HypaCas9

    Plasmid
    #138557
    Purpose
    Expresses human codon-optimized hyper-accurate SpCas9 and blasticidin resistance: EFS promoter-HypaCas9-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133
    Insert
    HypaCas9
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Mutation
    N692A, M694A, Q695A, H698A
    Promoter
    EFS
    Available since
    June 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138557-plasmid-map-sequence-id-274767

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  10. pDHt/sk-CE

    Plasmid
    #92128
    Purpose
    Expression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reesei
    Depositor
    Zhihua Zhou
    Publication
    Liu et al Cell Discov. 2015 May 12;1:15007. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-eGFP
    Use
    CRISPR; Trichoderma reesei expression
    Tags
    EGFP
    Promoter
    Pcbh2
    Available since
    Nov. 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    92128-plasmid-map-sequence-id-195044

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  12. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Publication
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  15. pCK002_U6-Sa-sgRNA(mod)_EFS-SaCas9-2A-Puro_WPRE

    Plasmid
    #85452
    Purpose
    Lentiviral vector encoding modified SaCas9 system backbone bearing BsmBI site for new guide RNAs and puromycin selection marker.
    Depositor
    Aviv Regev
    Publication
    Singer et al Cell. 2016 Sep 8;166(6):1500-1511.e9. do
    Insert
    U6-modified-sgRNA-Backbone-EFS-hSaCas9-2xNLS-2A-Puro
    Use
    CRISPR and Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Available since
    April 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    85452-plasmid-map-sequence-id-173969

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  16. pCMV-MMLVgag-3xNES-ABE8e-NG

    Plasmid
    #181754
    Purpose
    MMLV-Gag fused to 3xNES-ABE8e-NG with TSTLLMENSS cleavage site between Gag-NES and ABE. For production of virus like particles with base-editor RNP cargo.
    Depositor
    David Liu
    Publication
    Banskota et al Cell. 2022 Jan 7. pii: S0092-8674(21)014
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MMLVgag-3xNES-ABE8e-NG
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Feb. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    181754-plasmid-map-sequence-id-353937

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Showing: 1261 - 1280 of 15857 results