We narrowed to 9,961 results for: crispr plasmids
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Plasmid#115344PurposeSunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoterDepositorInsertgRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS
UseCRISPR and Synthetic BiologyExpressionPlantAvailable SinceMarch 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-LSD1-Hygro
Plasmid#104406Purposetransient expression of dCas9-LSD1 fusion proteinDepositorInsertLSD1 (KDM1A Human)
UseCRISPRExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTFE3-donor
Plasmid#112377PurposeCRISPR donor plasmid to tag human transcription factor TFE3 with GFPDepositorInsertTFE3 homology arms flanking EGFP-IRES-Neo cassette (TFE3 Human)
UseCRISPRMutationhg38:X:49030928:T>A,hg38:X:49030929:A>T,hg3…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
p206_LTJ_sgRNACD45.2_R3
Plasmid#82674PurposesgRNA targeting murine CD45.2 region 3. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting CD45.2 region 3
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
p184_LTJ_sgRNACD90.2
Plasmid#82670PurposesgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting mouse CD90.2
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-dCas9-LSD1-K661A-Hygro
Plasmid#104414Purposetransient expression of dCas9-dLSD1 fusion proteinDepositorInsertdLSD1 (KDM1A Human)
UseCRISPRExpressionMammalianMutationIRATp970A0364D (Source Bioscience) has a P405H ch…PromoterCMV promoterAvailable SinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
p183_LTJ_sgRNACD90.2_A
Plasmid#82671PurposesgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting CD90.2
UseCRISPRExpressionMammalianPromoterU6Available SinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMLX-donor
Plasmid#112370PurposeCRISPR donor plasmid to tag human transcription factor MLX with GFPDepositorInsertMLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
UseCRISPRMutationhg38:17:42570798:G>A, hg38:17:42570950:T>G,…Available SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTHRB-donor
Plasmid#112333PurposeCRISPR donor plasmid to tag human transcription factor THRB with GFPDepositorInsertTHRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
UseCRISPRAvailable SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF445-donor
Plasmid#112332PurposeCRISPR donor plasmid to tag human transcription factor ZNF445 with GFPDepositorInsertZNF445 homology arms flanking EGFP-IRES-Neo cassette (ZNF445 Human)
UseCRISPRMutationrs78585428, hg38:3:44446218:C>T, hg38:3:444466…Available SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pZNF785-donor
Plasmid#112317PurposeCRISPR donor plasmid to tag human transcription factor ZNF785 with GFPDepositorInsertZNF785 homology arms flanking EGFP-IRES-Neo cassette (ZNF785 Human)
UseCRISPRMutationhg38:16:30582292:A>T, hg38:16:30582302:A>T,…Available SinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
p240_LTJ_sgRNAFoxp3K276X
Plasmid#82675PurposesgRNA targeting murine Foxp3 mutant. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting Foxp3 K276X
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
p204_LTJ_sgRNACD45.2_R2
Plasmid#82673PurposesgRNA targeting murine CD45.2 region 2. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting CD45.2 region 2
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
p163_LTJ_sgRNACD90.1
Plasmid#82677PurposesgRNA targeting murine CD90.1. Co-expresses SpCas9-2A-EGFP.DepositorInsertsgRNA targeting mouse CD90.1
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-moxGFP
Plasmid#227273PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-moxGFP cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Puro-moxGFP
Plasmid#227275PurposeAAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Puro-mScarlet
Plasmid#227274PurposeAAVS1 targeting donor for the insertion of Puro and a strong EF1a promoter expressing mScarlet. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)DepositorInsertAAVS1 Homology Arms flanking a 2A-Puro-EF1a-mScarlet cassette (AAVS1 Synthetic)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mStayGold-TJP1
Plasmid#227300PurposeDonor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)DepositorInsertTJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H2BC11
Plasmid#207756PurposeDonor template for mScarlet insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755DepositorInsertH2BC11 Homology Arms flanking a mScarlet Tag (H2BC11 Human)
UseCRISPR; Donor templateExpressionMammalianAvailable SinceNov. 16, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-C-Solo-mStayGold-PXN
Plasmid#227316PurposeDonor template for mStayGold insertion into the C-terminus of the PXN locus. For focal adhesion visualization. To be co-transfected with sgRNA plasmid px330-PITCh-PXN (Addgene #227315)DepositorInsertPXN Homology Arms flanking a mStayGold Tag (PXN Human)
UseCRISPR; Donor templateExpressionMammalianPromoterPromoterlessAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only