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pFGC5941-PacI-ath-STTM2112-5p

Plasmid

#84109

Purpose

target miRNA2112-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM2112-5p

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM2112-3p

Plasmid

#84108

Purpose

target miRNA2112-3p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM2112-3p

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM2111a-5P/b-5P

Plasmid

#84107

Purpose

target miRNA2111 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM2111a-5P/b-5P

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM170

Plasmid

#84096

Purpose

target miRNA170 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM170

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM864-5p

Plasmid

#84184

Purpose

target miRNA864-5p for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM864-5p

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM823

Plasmid

#84152

Purpose

target miRNA823 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM823

Use

Tags

Expression

Plant

Mutation

Promoter

2x35S

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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HDAC1 Flag

Plasmid

#13820

Purpose

Mammalian expression of human histone deacetylase 1 with flag tag

Depositor

Eric Verdin

Article

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC1 (HDAC1 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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HDAC3 Flag

Plasmid

#13819

Purpose

Mammalian expression of human histone deacetylase 3 with flag tag

Depositor

Eric Verdin

Article

Emiliani et al Proc Natl Acad Sci U S A. 1998 Mar 17. 9

Insert

HDAC3 (HDAC3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pCAG Cas9-2A-Citrine

Plasmid

#92358

Purpose

CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

Cas9 2A Citrine

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA1-GATA6

Plasmid

#51929

Purpose

mammalian expression of mouse GATA6

Depositor

Jeffery Molkentin

Article

Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

Insert

GATA6 (Gata6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only
pX458-ECFP

Plasmid

#112220

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

ECFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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h-gamma-ENaC Myc

Plasmid

#83428

Purpose

full length human gamma ENaC with myc tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

Insert

human gamma ENaC (SCNN1G Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

P502A

Promoter

CMV

Available since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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h-alpha-ENaC

Plasmid

#83430

Purpose

full length human alpha ENaC with Flag tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

Insert

human alpha ENaC (SCNN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-Rheb

Plasmid

#19996

Depositor

Fuyuhiko Tamanoi

Article

Urano et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

Insert

Ras homolog enriched in brain 1 (RHEB Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 28, 2009

Availability

Academic Institutions and Nonprofits only

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h-beta-ENaC

Plasmid

#83429

Purpose

full length human beta ENaC with V5 tag

Depositor

Christie Thomas

Article

Raikwar et al Am J Physiol Renal Physiol. 2008 May;294

Insert

human beta ENaC (SCNN1B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pTK331

Plasmid

#59572

Purpose

TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

Depositor

Ron Weiss

Article

Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

Insert

SGP(16) 2xK-turn Kozak EGFP attB2 AscI

Use

Tags

Expression

Mutation

Promoter

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pSLQ7334: pHR-PGK-SV40_NLS-dCas12f-VPR-c- Myc_NLS-mCherry-WPRE

Plasmid

#176268

Purpose

Expression of dCas12f-VPR-mCherry in mammalian cells

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

dCas12f

Use

Lentiviral

Tags

HA-VPR-mCherry

Expression

Mammalian

Mutation

D326A, D510A

Promoter

PGK

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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LbCas12a-2C-NLS in pCSDest

Plasmid

#126638

Purpose

Expresses LbCas12a-2C-NLS in mammalian cell

Depositor

Scot Wolfe

Article

Liu et al Nucleic Acids Res. 2019 Mar 20. pii: 540

Insert

LbCas12a-2C-NLS

Use

CRISPR

Tags

3xHuman influenza hemagglutinin (HA)-Tag

Expression

Mammalian

Mutation

Promoter

CMV IE94 promoter

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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BC1209-pHR-dSV40-dCas9-14xGFP11-P2A-BFP

Plasmid

#164038

Purpose

Expression of dCas9-14xGFP11 in mammalian cells

Depositor

Baohui Chen

Article

Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

Insert

dCas9-14xGFP11

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A and H840A in Cas9

Promoter

dSV40

Available since

Jan. 28, 2021

Availability

Academic Institutions and Nonprofits only

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mCit-APPAPAPPAP-gs

Plasmid

#228091

Purpose

Mammalian expression of 10 coiled coil segments in APPAPAPPAP arrangement, with short flexible linkers and N-terminal mCitrine for phase separation and forming of liquid condensates in mammalian cells

Depositor

Roman Jerala

Article

Ramsak et al Nat Commun. 2023 Dec 2;14(1):7973. doi:

Insert

mCit, APPAPAPPAP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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