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We narrowed to 4,673 results for: pcr

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Showing: 1261 - 1280 of 4673 results

  7. pJJS005

    Plasmid
    #188328
    Purpose
    PCR template for repair templates to tag a gene with a mNeonGreen and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Publication
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    mIAA7::mNeonGreen::Linker
    Expression
    Bacterial
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188328-plasmid-map-sequence-id-370751

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  8. pJJS012

    Plasmid
    #188335
    Purpose
    PCR template for repair templates to tag a gene with a mTagBFP2 and mIAA7 AID degron using homology-directed repair for C. elegans.
    Depositor
    Mike Boxem
    Publication
    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660
    Insert
    Linker::mTagBFP2::mIAA7
    Expression
    Bacterial
    Available since
    Aug. 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    188335-plasmid-map-sequence-id-370742

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  12. pADH147

    Plasmid
    #90991
    Purpose
    NAT-marked "empty" gRNA construct for use in gRNA expression cassette stitching PCR. Use with pADH110 to generate target-specific part 2 of 2 for C.alb HIS1-FLP CRISPR system.
    Depositor
    Aaron Hernday
    Publication
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90991-plasmid-map-sequence-id-180524

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  13. BB3cN_pGAP_23*_pLAT1_Cas9

    Plasmid
    #104906
    Purpose
    hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    Feb. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    104906-plasmid-map-sequence-id-210667

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  17. pAG25-L-IFP-F[1] (clon 3)

    Plasmid
    #52899
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[1] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Publication
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    L-IFP-F[1]
    Use
    Vector for homologous recombination
    Promoter
    TEF promoter
    Available since
    June 12, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pAG32-L-IFP-F[2] (clon 3)

    Plasmid
    #52900
    Purpose
    Plasmid template for PCR of cassettes for HR, used to introduce IFP F[2] fragments 3’ to the ORF of the genes to study. Plasmid confers ampicillin resistance to DH5α.
    Depositor
    Stephen Michnick
    Publication
    Tchekanda et al Nat Methods. 2014 Jun;11(6):641-4. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    L-IFP-F[2]
    Use
    Vector for homologous recombination
    Promoter
    TEF promoter
    Available since
    June 5, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 1261 - 1280 of 4673 results