We narrowed to 54,958 results for: ran

1. pSems-leader-mEGFP-MBP-TMD(ALA7)

   Plasmid

   #193051

   Purpose

   Expression of artificial transmembrane protein tagged with mEGFP and MBP at the plasma membrane. Control construct for single molecule co-localization and co-tracking microscopy.

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   ALA7-TMD
   

   Tags

   Ig k-chain leader sequence, MBP, and mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(-)-mRho.V5.mER.hOr47a

   Plasmid

   #126472

   Purpose

   Human codon-optimized version of D. melanogaster Or47a (hOr47a) with N-terminal tags derived from human Rhodopsin (mRho) and HCN1 (mER) for improved intracellular trafficking in mammalian cells

   Depositor

   Bill Hansson

   Article

   Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

   Insert

   Or47a (Or47a Fly)
   

   Tags

   H. sapiens HCN1 106VNKFSL111 (mER), H. sapiens Rh…

   Expression

   Mammalian

   Mutation

   codon optimization for H. sapiens

   Promoter

   CMV

   Available Since

   June 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. PMP34-hLOV-TEVcs-GAL4bd-VP64-V5

   Plasmid

   #170997

   Purpose

   HiLITR transcription factor with full-length PMP34 targeting sequence (Peroxisome)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   PMP34(FL)-NNES-hLOV-TEVcs(ENLYFQ-M)-GAL4bd-VP64-V5 (SLC25A17 Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ef1-alpha

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1/Hygro-Mfn2-ActA

   Plasmid

   #191981

   Purpose

   Expression of Mfn2 tagged with mitochondrion localization signal (ActA)

   Depositor

   Luca Scorrano

   Insert

   Mfn2-ActA
   

   Tags

   ActA

   Expression

   Mammalian

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-PARL-D319A-FLAG C-t

   Plasmid

   #191958

   Purpose

   Expression of Presenilin Associated Rhomboid Like (PARL) D319A mutant

   Depositor

   Luca Scorrano

   Insert

   PARL D319A
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D319A

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP-Fis1-DTM

   Plasmid

   #191950

   Purpose

   Expression of a Fis1-GFP chimera lacking the C-terminal 27 residues (Fis1-DTM)

   Depositor

   Luca Scorrano

   Insert

   Fis1-DTM
   

   Expression

   Bacterial

   Mutation

   Fis1 gene lacking the C-terminal 27 residues

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCI-His-Fis1-DTM

   Plasmid

   #191946

   Purpose

   Expression of Fis1 lacking the C-terminal 27 residues (Fis1-DTM)

   Depositor

   Luca Scorrano

   Insert

   Fis1-DTM
   

   Expression

   Mammalian

   Mutation

   Fis1 gene lacking the C-terminal 27 residues

   Available Since

   Dec. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-DEST47-Noa1-GFP

   Plasmid

   #191968

   Purpose

   Gateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning Technology

   Depositor

   Luca Scorrano

   Insert

   Noa1-GFP
   

   Use

   Synthetic Biology

   Available Since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-∆Hapln1

    Plasmid

    #200778

    Purpose

    Expresses mouse HAPLN1 mutant lacking the lectican binding domain and fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Mutation

    deleted lectican binding domain (aminoacids 40-15…

    Promoter

    Synapsin

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV-HAPLN1

    Plasmid

    #200777

    Purpose

    Expresses full-length mouse HAPLN1 fused with cysteine-free GFP (cfGFP) under synapsin promoter

    Depositor

    Alexander Dityatev

    Article

    Ramsaran et al Science. 2023 May 5;380(6644):543-551. d

    Insert

    Hyaluronan And Proteoglycan Link Protein 1 (Hapln1 Mouse)
    

    Use

    AAV

    Tags

    cfGFP

    Promoter

    Synapsin

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV uN2C GFP (Ctl)

    Plasmid

    #224355

    Purpose

    Express GFP tagged upsteram ORF of NOTCH2NLC with a control size (12x) of GGC repeats

    Depositor

    Nicolas Charlet-Berguerand

    Article

    Boivin et al Neuron. 2021 Jun 2;109(11):1825-1835.e5.

    Insert

    uN2C
    

    Use

    AAV

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Codon-optimized for expression in human, so no pu…

    Promoter

    CMV + chimeric intyron (CAG)

    Available Since

    Oct. 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. AKAP1-hLOV-TEVcs-GAL4bd-VP64-V5

    Plasmid

    #170995

    Purpose

    HiLITR transcription factor with AKAP1 N-terminal tmd targeting sequence (mitochondria)

    Depositor

    Alice Ting

    Article

    Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

    Insert

    AKAP1(tmd)-NNES-hLOV-TEVcs(ENLYFQ/M)-GAL4bd-VP64-V5 (AKAP1 Synthetic)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    V5

    Expression

    Mammalian

    Promoter

    Ef1-alpha

    Available Since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCVpuro-Mito-DsRed1

    Plasmid

    #87379

    Purpose

    Retroviral transfection of cells to express DsRed1 (Ex555, Em585) localising to the mitochondrial matrix via the transit peptide from human TXN2 (Thioredoxin).

    Depositor

    Björn Stork

    Article

    Bohler et al Cell Death Dis. 2018 Feb 19;9(3):286. do

    Insert

    TXN2 mitochondrial transit peptide (TXN2 Human)
    

    Use

    Retroviral

    Tags

    DsRed1

    Expression

    Mammalian

    Mutation

    First 60 aa only

    Promoter

    Retroviral 5' LTR promoter

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDmelOR

    Plasmid

    #126476

    Purpose

    Vector for the functional expression of insect ORs in mammalian cells, with a human codon-optimized version of D.melanogaster Orco (hOrco) with an N-terminal myc tag and a β-globin/IgG chimeric intron

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Insert

    DmelOrco (Orco Fly)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    codon optimization for H. sapiens; includes a rec…

    Promoter

    CMV

    Available Since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHLsec hSOD1

    Plasmid

    #232481

    Purpose

    vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

    Depositor

    Lukas Trantirek

    Article

    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

    Insert

    Homo sapiens superoxide dismutase 1 (SOD1 Human)
    

    Tags

    DYKDDDDK (FLAG-tag)

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTRE3G-human cGASdeltaN-OMM

    Plasmid

    #186881

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain (503-541 a.a.) fusion (Adamts1 Human, Synthetic)
    

    Use

    Retroviral

    Mutation

    N-terminal truncation, MAVS transmembrane domain …

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTRE3G-human cGASdeltaN-Pex

    Plasmid

    #186882

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with C-terminal PEX13 transmembrane domain (136-233 a.a.) fusion (Adamts1 Human, Synthetic)
    

    Use

    Retroviral

    Mutation

    N-terminal truncation, PEX13 transmembrane domain…

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRE3G-human cGASdeltaN-ER

    Plasmid

    #186883

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with C-terminal VAMP2 transmembrane domain (86-119 a.a.) fusion (Adamts1 Human, Synthetic)
    

    Use

    Retroviral

    Mutation

    N-terminal truncation, VAMP2 transmembrane domain…

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-CMV-human cGASdeltaN-OMM

    Plasmid

    #186887

    Purpose

    Expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS truncation mutant (160-522 a.a.) with C-terminal MAVS transmembrane domain fusion (Adamts1 Human, Synthetic)
    

    Use

    Lentiviral

    Mutation

    N-terminal truncation, MAVS transmembrane domain …

    Promoter

    CMV

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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