We narrowed to 2,056 results for: ENDOG

1. pSLPB3B-mAID-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin-T2A-osTIR1

   Plasmid

   #187963

   Purpose

   mAID degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance, T2A site and osTIR1 under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Inserts

   AID-SpdCas9-tagRFPt-P2A-tagBFP; Blasticidin-T2A-osTIR1
   osTIR1
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker and T2A

   Expression

   Mammalian

   Available Since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAav-TP53-T2A-BirA*

   Plasmid

   #115652

   Purpose

   rAAV-based donor template for genome engineering of the TP53 protein C-terminus containing a T2A-BirA* module and a selection cassette

   Depositor

   Sven Eyckerman

   Article

   Vandemoortele et al J Proteome Res. 2018 Dec 10. doi: 10.102

   Inserts

   TP53 (TP53 Human)
   TP53 (TP53 Human)
   T2A
   BirA*
   

   Use

   AAV

   Tags

   BirA* and T2A

   Mutation

   Homology region 1 and Homology region 2

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-Basic-ACSL3-sfGFP(C) HDR template

   Plasmid

   #129413

   Purpose

   HDR tempalte for tagging of endogenous human ACSL3 C-terminus with sfGFP

   Depositor

   Elina Ikonen

   Article

   Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

   Insert

   ACSL3 HDR template (ACSL3 Human)
   

   Use

   CRISPR and TALEN; Endogenous tagging hdr template

   Tags

   sfGFP

   Expression

   Mammalian

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. KI(GAPDH)-P2A-EGFP-CMV-NeoR

   Plasmid

   #114009

   Purpose

   HDR-cassette to target chicken GAPDH gene for EGFP expression under control of the endogenous promoter. The cassette contains NeoR gene for positive clone selection and DTA gene for negative selection

   Depositor

   Pavel Volchkov

   Article

   Antonova et al F1000Res. 2018 Feb 28;7:238. doi: 10.126

   Inserts

   GAPDH left arm (GAPDH Chicken)
   eGFP
   NeoR
   GAPDH right arm
   

   Expression

   Mammalian

   Promoter

   -, CMV, and endogenous promoter (chicken GAPDH in…

   Available Since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-SEPT_Cdk2-T160E-siR

   Plasmid

   #73976

   Purpose

   HDR template to generate Cdk2-T160E-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160E (CDK2 Human)
   

   Use

   AAV

   Mutation

   T160 mutated to Glu and siRNA target site mutated

   Available Since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1+FLAG-KrascomQ61R

   Plasmid

   #206845

   Purpose

   To express mouse Kras encoded by common mouse codons (to increase expression) and with a Q61R mutation with an N-terminal FLAG tag.

   Depositor

   Christopher Counter

   Article

   Le Roux et al Elife. 2022 Sep 7;11:e75715. doi: 10.755

   Insert

   Kras (Kras Mouse)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Codons altered to most optimum based on mouse cod…

   Available Since

   July 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Calb1-T2A-dgCre targeting vector

   Plasmid

   #114438

   Purpose

   Target dgCre into the endogenous mouse Calb1 locus at the stop codon

   Depositor

   Hongkui Zeng

   Article

   Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

   Insert

   dgCre
   

   Use

   Mouse Targeting

   Tags

   DHFR domain and EGFP

   Promoter

   none; utilizes endogenous Calb1 promoter for expr…

   Available Since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCF806-shABC1

   Plasmid

   #186713

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Oct. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCF806-shABC2

   Plasmid

   #186714

   Purpose

   Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.

   Depositor

   Christof Fellmann

   Article

   Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

   Insert

   ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EF1a-HIF2a dPA (P405A, P531A)

    Plasmid

    #232954

    Purpose

    Stably express a constitutively stable version of HIF2a (HIF2a dPA)

    Depositor

    William Kaelin

    Article

    Jiang et al Cell. 2025 Feb 25:S0092-8674(25)00156-4.

    Insert

    HIF2a (EPAS1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P405A, P531A

    Promoter

    EF1a

    Available Since

    March 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. makeTCR_HDR_Hs.TRAC_Backbone

    Plasmid

    #233944

    Purpose

    backbone for makeTCR assembly

    Depositor

    Michael Platten

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    knock-in to endogenous TRAC

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRY003

    Plasmid

    #81043

    Purpose

    HO endonuclease expression

    Depositor

    John McCusker

    Insert

    HO
    

    Expression

    Yeast

    Promoter

    endogenous

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pUC19_GZG

    Plasmid

    #117226

    Purpose

    shble flanked by an endogenous GAPDH promoter and terminator system

    Depositor

    Jackie Collier

    Insert

    shble
    

    Use

    Thraustochytrid expression

    Promoter

    GAPDH

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. BRR2 pRS313

    Plasmid

    #111411

    Purpose

    Brr2 (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Available Since

    Oct. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. hSLBP_mNG_FS_Donor

    Plasmid

    #191387

    Purpose

    Donor for C-terminal tagging of endogenous hSLBP

    Depositor

    Yannick Doyon

    Insert

    mNeonGreen(mNG) + FS(3xFLAG_TwinStrep)Tag for hSLBP tagging at the C-terminus (SLBP Human, Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. brr2-C520R pRS313

    Plasmid

    #111412

    Purpose

    brr2-C520R (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    C520R aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. brr2-N1104A pRS313

    Plasmid

    #111414

    Purpose

    brr2-N1104A (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    N1104A aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. brr2-R1107L pRS313

    Plasmid

    #111416

    Purpose

    brr2-R1107L (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    R1107L aa mutation

    Available Since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. brr2-Q904E pRS313

    Plasmid

    #111413

    Purpose

    brr2-Q904E (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    Q904E aa mutation

    Availability

    Academic Institutions and Nonprofits only

    

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20. brr2-R1107A pRS313

    Plasmid

    #111415

    Purpose

    brr2-R1107A (endogenous promoter)

    Depositor

    Christine Guthrie

    Article

    Mayerle et al bioRxiv 2018. 354514

    Insert

    BRR2
    

    Expression

    Yeast

    Mutation

    R1107A aa mutation

    Availability

    Academic Institutions and Nonprofits only

    

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