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pCRII-1.1-1.10a

Plasmid

#69589

Purpose

OT-II TCR a chain cDNA in pCRII

Depositor

Francis R. Carbone

Article

Barnden et al Immunol Cell Biol. 1998 Feb;76(1):34-40.

Insert

TCR alpha clone 1.1 (Tcra Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
pGL3-CMV-p85a 3'UTR

Plasmid

#51392

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

p85alpha 3'UTR (PIK3R1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
GFP-mZO-1(9-1665)-Myc-His

Plasmid

#236530

Purpose

For expression of ZO-1 (9-1665 aa, mouse)-Myc-His alpha- in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al Tissue Barriers. 2019;7(3):e1653748. doi

Insert

TJP1 (Tjp1 Mouse)

Use

Tags

GFP and Myc-His

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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GFP-mZO-1(9-1745)-Myc-His

Plasmid

#236529

Purpose

For expression of ZO-1 (9-1745 aa, mouse)-Myc-His alpha+ in mammalian cells

Depositor

Sandra Citi

Article

Rouaud et al Tissue Barriers. 2019;7(3):e1653748. doi

Insert

TJP1 (Tjp1 Mouse)

Use

Tags

GFP and Myc-His

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-mEGFP-PKCa-C1

Plasmid

#112264

Purpose

ITRACKalpha Donor Construct

Depositor

Ryohei Yasuda

Article

Colgan et al Nat Neurosci. 2018 Aug;21(8):1027-1037.

Insert

protein kinase C alpha (PRKCA Bovine)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

A11V (please see depositor comments below)

Promoter

CMV

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-CMV-p85a 3'UTR mutant

Plasmid

#51393

Depositor

Narry Kim

Article

Park et al Nat Struct Mol Biol. 2009 Jan;16(1):23-9

Insert

p85alpha 3'UTR (PIK3R1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mutation

miR-29 binding site mutant

Promoter

CMV

Available since

March 31, 2014

Availability

Academic Institutions and Nonprofits only
hDAGLa-V5

Plasmid

#87676

Purpose

Expresses human DAGL? protein with intracellular V5 tag located on the C-terminal

Depositor

Pat Doherty

Article

Singh et al Biosci Rep. 2016 May 6;36(3). pii: e0033

Insert

Human Diacylglycerol Lipase Alpha (DAGLA Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

Promoter

T7

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA

Plasmid

#12522

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pBabe puro Myr HA PIK3CA

Plasmid

#12523

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA Myr (PIK3CA Human)

Use

Retroviral

Tags

HA and Myr

Expression

Mammalian

Mutation

Myristoylation. Constitutively active allele; I14…

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA E545K

Plasmid

#12525

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA E545K (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

E545K. Tumor-derived allele.

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA-FLAG-MYO1D

Plasmid

#125125

Purpose

Expresses human FLAG-MYO1D

Depositor

Bruce Beutler

Article

McAlpine et al Dis Model Mech. 2018 Sep 27;11(9). pii:

Insert

MYO1D (MYO1D Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-UBC-hAAT-IRES-ZsGreen-W

Plasmid

#126702

Purpose

lentiviral bicistronic exprression of both human alpha-1 anitrypsin and ZsGreen

Depositor

Darrell Kotton

Article

Wilson et al Am J Respir Cell Mol Biol. 2008 Aug . 39

Insert

Alpha-1 antitrypsin (hAAT aka SERPINA1) and ZsGreen; "AAT-IRES-ZSGreen" (SERPINA1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UBC

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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SERPINA-bio-His

Plasmid

#52182

Purpose

Expresses full-length Alpha-1-antitrypsin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

SERPINA (SERPINA1 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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PCDH-bio-His

Plasmid

#53336

Purpose

Expresses full-length Protocadherin alpha-2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

PCDH (PCDHA2 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-a4-delta tail

Plasmid

#80018

Purpose

Mammalian expression of human integrin alpha4 with no tail

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

integrin alpha4 (ITGA4 Human)

Use

Tags

Expression

Mammalian

Mutation

no cytoplasmic tail (amino acids 1 to 1007)

Promoter

CMV

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pBabe puro HA PIK3CA H1047R

Plasmid

#12524

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA H1047R (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

H1047R. Tumor-derived allele.

Promoter

Available since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pBiex PKC - Peptide 14 mCit mCer

Plasmid

#83565

Purpose

Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the pseudosubstrate of PKCalpha.

Depositor

Sivaraj Sivaramakrishnan

Article

Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

Insert

PKC alpha (PRKCA Human)

Use

Tags

10 nm ER/K Helix, FLAG Purificaiton Tag, Tev Prot…

Expression

Bacterial and Insect

Mutation

Only catalytic domain of PKC (aa 335-672)

Promoter

T7 Promoter

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pg-ODD-EGFP

Plasmid

#87211

Purpose

HIF-1a's ODD domain fused to EGFP

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

HIF-1a ODD domain (HIF1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

ODD domain (AA 530-652)

Promoter

CMV

Available since

March 13, 2017

Availability

Academic Institutions and Nonprofits only

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pRK172-α-syn-TEV-GFP

Plasmid

#166671

Purpose

Bacterial expression of alpha synuclein coupled to GFP via a TEV site for uptake studies

Depositor

Iris Lindberg

Article

Jarvela et al PLoS One. 2021 Jan 26;16(1):e0241161. do

Insert

mouse synuclein (from V. Lee) (Snca Mouse)

Use

Tags

GFP

Expression

Bacterial

Mutation

added a TEV cleavage site sequence between synucl…

Promoter

CMV

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pIE-hα2M-H6

Plasmid

#128492

Purpose

Expresses human α2M (from S24 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (A2M Human)

Use

Tags

His tag (6x)

Expression

Insect

Mutation

Promoter

IE1

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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