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Plasmid#234130PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 27bp dist
Plasmid#234129PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 15bp dist
Plasmid#234128PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 42bp dist
Plasmid#234127PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 50bp dist
Plasmid#234112PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
ACT1-CUP1 46bp dist
Plasmid#234111PurposePCR to add remove nucleotides within intron of ACT1-CUP1 insert then restriction digest and ligation of new insert to backboneDepositorInsertACT1-CUP1
UseTagsExpressionYeastMutationIntron truncationPromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_3V46
Plasmid#234165PurposeHeterologous protein expression of cell division control protein 73 in Escherichia coliDepositorInsert3V46 (CDC73 Budding Yeast)
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRS313_MSN5-LY00045
Plasmid#232904PurposePlasmid carrying MSN5 from LY00045 (S288C) with its native promoter and terminator.DepositorInsertMSN5 (MSN5 Budding Yeast)
UseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-SkA
Plasmid#219977PurposeeIF4G-SkA protein purificationDepositorInserteIF4G-SkA (TIF4631 Budding Yeast)
UseTags6xHis-MBPExpressionInsectMutationeIF4A binding domain of S. kudriavzeviiPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOCC102-MBP-eIF4G-RAR
Plasmid#219973PurposeeIF4G-RAR protein purificationDepositorInserteIF4G-RAR (TIF4631 Budding Yeast)
UseTags6xHis-MBPExpressionInsectMutationTruncation: amino acids 490 to 942PromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHflu3
Plasmid#203881PurposepHI1 with insertion of kanamycin-resistance geneDepositorInsertaminoglycoside O-phosphotransferase APH(3')-IIa
UseSynthetic BiologyTagsExpressionBacterial and YeastMutationPromoterAvailable sinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAS-mak10 gRNA
Plasmid#160385PurposeExpresses guide RNA for targeting MAK10 in yeastDepositorInsertmak10 gRNA
UseTagsExpressionYeastMutationPromoterRNA pol III promoter (tRNA-Tyr)Available sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
KBB258
Plasmid#185072PurposeBacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusionDepositorInsertNUP1
UseTagsGSTExpressionYeastMutationNup1 N-term+FXFG repeatsPromoterAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
HCP7
Plasmid#166109PurposeThis plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Whi5 and the other targets the C-terminus of Hta2.DepositorInsertWhi5-sg106 (WHI5 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LHP809 - Ub-K63A-CYC1
Plasmid#32166DepositorUseTagsExpressionYeastMutationK63APromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
LHP327 - Ub-F45A/G76A
Plasmid#32165DepositorUseTagsExpressionYeastMutationF45A and G76APromoterCUP1Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTH339-SUP45(1-138)-GalBD
Plasmid#29739DepositorInsertSUP45 (SUP45 Budding Yeast)
UseTagsGal Activation domain and MycExpressionYeastMutationCodons 1-138 of the yeast SUP45 gene onlyPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107M pRS313
Plasmid#111417Purposebrr2-R1107M (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationR1107M aa mutation (note, not an RP allele)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pAS161
Plasmid#194484PurposesynER CassetteDepositorInsertsynER Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGR595
Plasmid#213785PurposeIntegrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.DepositorInsertBadBoy2 TP-DNAP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.2_mCherry_roGFP2_Orp1
Plasmid#155043PurposeFluorescent redox reporterDepositorInsertOrp1 (HYR1 Budding Yeast)
UseLentiviralTagsroGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pAG60
Plasmid#35128DepositorInsertURA3
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceSept. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAS168
Plasmid#194491PurposeZif268 Op (Part 2)DepositorInsertZif268 Op (Part 2)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAS163
Plasmid#194486PurposesynMR CassetteDepositorInsertsynMR Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSII426
Plasmid#35470DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBPZpGAL4DBDUw
Plasmid#26233DepositorInsertZip-GAL4DBD
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 7, 2010AvailabilityAcademic Institutions and Nonprofits only -
p426TEF-LptTPS-A
Plasmid#89468PurposeExpression of prespatane synthase LptTPS-A in S. cerevisiae for sesquiterpene productionDepositorInsertLptTPS-A
UseTagsnoneExpressionYeastMutationPromoterpBR322 origin of replication for propagation in E…Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAS165
Plasmid#194488Purpose97-4 Op (Part 2)DepositorInsert97-4 Op (Part 2)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSII423
Plasmid#35464DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only