2,339 results

Showing: 1921 - 1950 of 2339 results

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CamKII-LGI1-S473L-pHluorin

Plasmid

#185540

Purpose

Expresses mutant S473L LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed serine 473 for leucine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-Y433A-pHluorin

Plasmid

#185541

Purpose

Expresses mutant Y433A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed tyrosine 433 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-R474Q-pHluorin

Plasmid

#185542

Purpose

Expresses mutant R474Q LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed arginine 474 for glutamine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-T380A-pHluorin

Plasmid

#185543

Purpose

Expresses mutant T380A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed threonine 380 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-E383A-pHluorin

Plasmid

#185544

Purpose

Expresses mutant E383A LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed glutamic acid 383 for alanine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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CamKII-LGI1-C200R-pHluorin

Plasmid

#185545

Purpose

Expresses mutant C200R LGI1-pHluorin under the CamKII promoter

Depositor

Jaime de Juan-Sanz

Article

Cuhadar et al Cell Rep. 2024 May 28;43(5):114186. doi:

Insert

LGI1 (Lgi1 Rat)

Use

Lentiviral

Tags

pHluorin

Expression

Mammalian

Mutation

changed cysteine 383 for arginine

Promoter

CamKII

Available Since

May 3, 2024

Availability

Academic Institutions and Nonprofits only

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rSirt3 shRNA (pLKO.1 vector)

Plasmid

#216807

Purpose

shRNA against rat Sirt3

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al J Cell Biol. 2024 Jan 1;223(1):e20230504

Insert

Sirt3 (Sirt3 Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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hSLC17A6/vGlutT2/pCMV6-entry

Plasmid

#196939

Purpose

Plasmid expressing an antigen targeting the VGluT2 transporter

Depositor

James Trimmer

Insert

vGlutT2/ SLC17A6

Expression

Mammalian

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 (96-421)

Plasmid

#213619

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET SUMO-syt1 (96-421) I367C

Plasmid

#213620

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) I367C (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

C277A; I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 5W

Plasmid

#213623

Purpose

Bacterial expression of SUMO-tagged rat syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET SUMO-syt1 s7+

Plasmid

#213624

Purpose

Bacterial expression of SUMO-tagged rat syt1 s7+

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 s7+ (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

L171K, H198K, K236R, C277A, N333R, Y364K, I 367C,…

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1 5W

Plasmid

#213629

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET SUMO-Doc2β C2AB (G361C)

Plasmid

#213631

Purpose

Bacterial expression of SUMO-tagged rat Doc2β C2AB (G361C)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

Doc2β C2AB (G361C) (Doc2b Rat)

Tags

SUMO

Expression

Bacterial

Mutation

G361C, all original cysteines mutated to alanines

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a_Caspase-11_rat_catalytic domain

Plasmid

#214329

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

CASP4 (Casp4 Rat)

Tags

His-TEV

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_Pro-IL-18_rat

Plasmid

#214333

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (Il18 Rat)

Tags

His-TEV and Myc

Expression

Bacterial

Promoter

T7

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBMeCP2G.10 (pc1577)

Plasmid

#211728

Purpose

Expresses MeCP2 (aa1-76) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa1-76

Tags

GFP

Expression

Insect

Promoter

Polyhedrin promotor

Available Since

Feb. 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-MeCP2.9Ch (pc2385)

Plasmid

#211720

Purpose

Expresses MeCP2 (aa163-309) mCherry tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Tags

mCherry

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-MeCP2G.9 (pc1574)

Plasmid

#211719

Purpose

Expresses MeCP2 (aa163-309) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-MeCP2G.8 (pc1573)

Plasmid

#211718

Purpose

Expresses MeCP2 (aa310-492) GFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa310-492

Tags

GFP

Expression

Insect

Promoter

Polyhedrin Promoter

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB-MeCP2Y.3 (pc1575)

Plasmid

#211717

Purpose

Expresses MeCP2 (aa1-163) YFP tagged in insect cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa1-163

Tags

YFP

Expression

Insect

Available Since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMeCP2R.9 (pc2812)

Plasmid

#211574

Purpose

Expresses MeCP2 (aa163-309) GFP tagged in mammalian cells

Depositor

Cristina Cardoso

Article

Becker et al PLoS One. 2013;8(1):e53730. doi: 10.1371

Insert

MeCP2 aa163-309

Tags

RFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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CavB4/pFlag myc-CMV

Plasmid

#197302

Purpose

Plasmid expressing an antigen targeting the Cavbeta4 calcium channel

Depositor

Ricardo Dolmetsch

Insert

Cavb4

Tags

pFLAGmyc

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pETSUMOSyt1C2AB(80-421)

Plasmid

#209005

Purpose

Recombinant protein expression of truncated syt1 construct

Depositor

Edwin Chapman

Article

Mehta et al Nat Commun. 2024 Jan 4;15(1):262. doi: 1

Insert

syt1 (Syt1 Rat)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

aa 80-421 only

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
dCas9-RPT6-FLAG

Plasmid

#205416

Purpose

Expresses dCas9-RPT6 with FLAG label

Depositor

Timothy Jarome

Article

Farrell et al J Neurosci. 2024 Jan 24;44(4):e145323202

Insert

dCas9-RPT6 (Psmc5 Rat)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
An R57A, R59A CAAX pMT2

Plasmid

#206119

Purpose

Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Mutation

R57A, R59A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
cacna2d1 R241A pMT2

Plasmid

#206110

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit and with R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
cacna2d1 R241A HA pMT2

Plasmid

#206111

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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