2,226 results

1. pGADT7-AP-2 σ2

   Plasmid

   #198345

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 σ2 fusion protein in yeast (yeast two-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Mol Biol Cell. 2022 Oct 1;33(12):ar109.

   Insert

   AP-2 σ2
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   R42G substitution, and silent substitutions in co…

   Promoter

   ADH1

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEPOZ0CM0137

   Plasmid

   #197535

   Purpose

   Phytobricks Level 0 part

   Depositor

   Nicola Patron

   Article

   Cai et al ACS Synth Biol. 2023 Nov 17;12(11):3482-

   Insert

   C-terminal glucocorticoid receptor, GR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMeCP2G pc1121

   Plasmid

   #167566

   Purpose

   The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

   Depositor

   Cristina Cardoso

   Article

   Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

   Inserts

   Methyl-CpG binding protein 2 (Mecp2 Rat)
   GFP
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMeCP2miRFP670 pc3942

   Plasmid

   #167568

   Purpose

   The miRFP670-gene is ligated c-terminally to the rat meCP2-gene.

   Depositor

   Cristina Cardoso

   Article

   Chagin et al Nucleus. 2019 Dec;10(1):231-253. doi: 10

   Insert

   MeCp2 (Mecp2 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMeCP2Y pc644

   Plasmid

   #167565

   Purpose

   The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced YFP (pEYFP-N1 vector; CLONTECH Laboratories, Inc.)

   Depositor

   Cristina Cardoso

   Article

   Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

   Insert

   Methyl-CpG binding protein 2 (Mecp2 Rat)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMyosin-miRFP718nano

   Plasmid

   #195748

   Purpose

   LC-Myosin labelled with near-infrared fluorescent protein miRFP718nano

   Depositor

   Vladislav Verkhusha

   Article

   Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

   Insert

   miRFP718nano-LC-Myosin
   

   Use

   Tags

   miRFP718nano

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. PH-miRFP-FRBx2

   Plasmid

   #193569

   Purpose

   Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   PH domain from PLC delta1 (Plcd1 Rat)
   

   Use

   Tags

   FRB (T2098L) and miRFP703

   Expression

   Mammalian

   Mutation

   PH domain

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PH-mCherry-FRBx2

   Plasmid

   #193570

   Purpose

   Expresses PH domain from PLC delta1 tagged with mCherry and two FRB mutants (T2098L) in mammalian cells.

   Depositor

   Justin Taraska

   Article

   Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

   Insert

   PH domain from PLC delta1 (Plcd1 Rat)
   

   Use

   Tags

   FRB (T2098L) and mCherry

   Expression

   Mammalian

   Mutation

   PH domain

   Promoter

   CMV

   Available since

   Feb. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-fDIO-mScarlet-Gphn_C4a

   Plasmid

   #194973

   Purpose

   AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4a) under the control of human Synapsin promoter

   Depositor

   Günter Schwarz

   Article

   Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

   Insert

   mScarlet-Gphn (isoform C4a) (Gphn Rat, Synthetic)
   

   Use

   AAV

   Tags

   mScarlet

   Expression

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-hSyn-fDIO-mScarlet-Gphn_C4d

    Plasmid

    #194975

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4d) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4d) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-hSyn-fDIO-mEGFP-Gphn_P1

    Plasmid

    #194978

    Purpose

    AAV vector to drive the Flp-dependent expression of mEGFP (L221K) -Gphn (isoform P1) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mEGFP-Gphn (isoform P1) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-hSyn-fDIO-mScarlet-Gphn_P1

    Plasmid

    #194972

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform P1) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform P1) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-hSyn-fDIO-mScarlet-Gphn_C4c

    Plasmid

    #194974

    Purpose

    AAV vector to drive the Flp-dependent expression of mScarlet-Gphn (isoform C4c) under the control of human Synapsin promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    mScarlet-Gphn (isoform C4c) (Gphn Rat, Synthetic)
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PVALB mAb_BCN14.1.1C9_LC_Rat Ig Kappa

    Plasmid

    #182891

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PVALB mAb_BCN14.1.1A2_HC_Rat IgG2a

    Plasmid

    #182894

    Purpose

    Mammalian expression of PVALB rat mAb HC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) heavy chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PVALB mAb_BCN14.1.1A2_LC_Rat Ig Kappa

    Plasmid

    #182895

    Purpose

    Mammalian expression of PVALB rat mAb LC gene

    Depositor

    Heng Zhu

    Insert

    anti-PVALB (human) light chain (PVALB Rat)
    

    Use

    Affinity Reagent/ Antibody

    Tags

    Expression

    Mammalian

    Mutation

    No

    Promoter

    CMV

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. mCherry-7aa-cb5-pEGFP-C1

    Plasmid

    #177407

    Purpose

    To express mCherry fused to endoplasmic reticulum (ER) targeting sequence "Cb5". mCherry faces the cytosol.

    Depositor

    David Andrews

    Insert

    Cb5 tail anchor (Cyb5r4 Rat)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1_rSERT_C109A_Y107C

    Plasmid

    #190174

    Purpose

    For expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathway

    Depositor

    Gary Rudnick

    Article

    Jacobs et al J Biol Chem. 2007 Oct 5;282(40):29441-7.

    Insert

    SLC6A4 Serotonin Transporter (Slc6a4 Rat)
    

    Use

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    changed Cys109 to alanine, changed tyr107 to cyst…

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1_rSERT_X5C_S277C

    Plasmid

    #190177

    Purpose

    For mammalian cell expression of rat SERT and measurement of cytoplasmic pathway accessibility

    Depositor

    Gary Rudnick

    Article

    Zhang et al J Biol Chem. 2006 Nov 24;281(47):36213-2

    Insert

    rat serotonin transporter (Slc6a4 Rat)
    

    Use

    Tags

    FLAG, His6, and c-myc

    Expression

    Mammalian

    Mutation

    Cys15Ala, Cys21Ala, Cys109Ala, Ser277Cys, Cys357I…

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-C1-3x-NLS-mScarlet-AMPKα2

    Plasmid

    #192452

    Purpose

    mScarlet-tagged alpha2 isoform of AMPK catalytic subunit targeted to the nucleus.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    3x-NLS-mScarlet-AMPKalpha2 (Prkaa2 Rat)
    

    Use

    Tags

    3x Nuclear localization signal (NLS) and mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFP-C1-mScarlet-AMPKα2

    Plasmid

    #192451

    Purpose

    mScarlet-tagged alpha2 isoform of AMPK catalytic subunit.

    Depositor

    Jin Zhang

    Article

    Schmitt et al Nat Commun. 2022 Jul 5;13(1):3856. doi:

    Insert

    mScarlet-AMPKalpha2 (Prkaa2 Rat)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV_pMBP-dnVamp3-P2AT2A-EGFP-caax

    Plasmid

    #190154

    Purpose

    Expresses dominant-negative Vamp3 (rat Vamp3 AA 1-81) plus membrane-targeted EGFP in oligodendrocytes; AAV vector

    Depositor

    Brad Zuchero

    Article

    Lam et al Nat Commun. 2022 Sep 23;13(1):5583. doi:

    Insert

    Vamp3 (Vamp2 Rat)
    

    Use

    AAV

    Tags

    P2AT2A-EGFP-caax

    Expression

    Mammalian

    Mutation

    Truncation that includes only rat Vamp3 AA #1-81

    Promoter

    MBP

    Available since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. RAT +D122H

    Plasmid

    #187426

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed aspartic acid at 129 for histidine

    Promoter

    PH and p10

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. RAT +R111E

    Plasmid

    #187425

    Purpose

    Expresses Na,K-ATPase (ATP1A1 and ATP1B1 in pFastBac Dual vector) in insect cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al bioRxiv 2021.11.29.470343

    Inserts

    ATP1A1 (Atp1a1 Rat)
    ATP1B1 (Atp1b1 Rat)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    changed arginine at 118 to glutamic acid

    Promoter

    PH and p10

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. VAMP8

    Plasmid

    #186689

    Purpose

    Expresses VAMP8 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    VAMP8
    

    Use

    Tags

    3xFLAG, GST, TEV, and Thrombin

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. EGFP-Myo1b motor

    Plasmid

    #187364

    Purpose

    Expresses rat myosin 1b isoform b motor domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

    Insert

    myosin 1b isoform b motor domain (Myo1b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Stx3

    Plasmid

    #186685

    Purpose

    Expresses Syntaxin 3 in E. Coli

    Depositor

    Axel Brunger

    Article

    Lai et al Nature. 2022 Mar;603(7903):949-956. doi:

    Insert

    Syntaxin 3
    

    Use

    Tags

    6 his tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. EGFP-Myo1b-Tail

    Plasmid

    #187365

    Purpose

    Expresses rat myosin 1b isoform b Tail domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Prosperi et al J Cell Biol. 2015 Jul 20;210(2):347-61.

    Insert

    myosin 1b isoform b Tail domain (Myo1b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. EGFP-Myo1-IQ-Tail

    Plasmid

    #187363

    Purpose

    Expresses rat myosin 1b isoform b IQTail domain labelled with EGFP

    Depositor

    Evelyne Coudrier

    Article

    Raposo et al Mol Biol Cell. 1999 May . 10(5):1477-94.

    Insert

    myosin 1b isoform b IQTail domain (Myo1b Rat)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-TetO(3G)-GCaMP6f-WPRE

    Plasmid

    #186205

    Purpose

    Express GCaMP6f under TetO(3G)

    Depositor

    Naoshige Uchida

    Article

    Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    6xHis (N terminal on insert), T7 epitope, and Xpr…

    Expression

    Mutation

    Promoter

    TetO(3G)

    Available since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge