We narrowed to 11,674 results for: NSI

1. pTSSX-STC

   Plasmid

   #115668

   Purpose

   E.coli expression plasmid of small tetraheme cytochrome (STC) from Shewanella oneidensis MR-1 with cleavable Strep tag

   Depositor

   David Kramer

   Article

   Huang et al J Am Chem Soc. 2020 Jun 10;142(23):10459

   Insert

   small tetraheme cytochrome c
   

   Tags

   Signal peptide (aa1-27)-Strep-II tag- Factor Xa c…

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBabe-PI(WT)-Src(YF)-mCherry

   Plasmid

   #87357

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; YF: Y535F const. active mutation

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src isoform 2 (Src Mouse)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Y535F

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBabe-PI(WT)-Src(KD)-mCherry

   Plasmid

   #87358

   Purpose

   PI(WT): Photo-inhibitable/blue light sensitive; kinase dead Src mutation

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   Src (Src Mouse)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   D388R

   Promoter

   CMV

   Available Since

   April 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGCNK58

   Plasmid

   #202461

   Purpose

   Plasmid for the expression of the basic region and leucine zipper domain of the Saccharomyces cerevisiae transcription factor GCN4 in bacterial cells

   Depositor

   Arthur Palmer, Stephen Harrison

   Article

   Weiss et al Nature. 1990 Oct 11;347(6293):575-8. doi

   Insert

   Residues 226-281 of Sacchromyces cerevisiae GCN4 plus an N-terminal Met-Lys extension (GCN4 Budding Yeast)
   

   Expression

   Bacterial

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUE1-TSP-ORF68 R443A

   Plasmid

   #162652

   Purpose

   Expresses ORF68 R443A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   ORF68
   

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Amino acid R443 mutated to alanine

   Promoter

   CMV

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. RS18960_pMQ30_KO_Kan

   Plasmid

   #185393

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS18960 upstream flanking homology region
   RR42_RS18960 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. RS17060_pMQ30_KO_Kan

   Plasmid

   #185396

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS17060 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS17060 upstream flanking homology region
   RR42_RS17060 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RS17055_pMQ30_KO_Kan

   Plasmid

   #185398

   Purpose

   Non-replicating vector used to create markerless deletion of RR42_RS17055 in C. basilensis 4G11

   Depositor

   Adam Arkin

   Article

   Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

   Inserts

   RR42_RS17055 upstream flanking homology region
   RR42_RS17055 downstream flanking homology region
   

   Available Since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. K8.1Pr-pGL4.16

   Plasmid

   #131037

   Purpose

   Firefly Luciferase reporter for K8.1 promoter from KSHV

   Depositor

   Britt Glaunsinger

   Article

   Nandakumar et al PLoS Pathog. 2019 May 16;15(5):e1007774.

   Insert

   KSHV K8.1 100 bp promoter
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEF-XMAS-2xStop

    Plasmid

    #164412

    Purpose

    Transient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.

    Depositor

    Xiao Wang

    Article

    Brookhouser et al BMC Biol. 2020 Dec 14;18(1):193. doi: 10

    Insert

    mCherry
    

    Use

    CRISPR and Synthetic Biology

    Tags

    T2A-GFP

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Aug. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGST-TEV-yVms1(1-417,delta(38-69))

    Plasmid

    #128739

    Purpose

    Bacterial overexpression and crystallization

    Depositor

    Chris Hill

    Article

    Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

    Insert

    Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    residues 1-417 with deletion of 38-69

    Promoter

    T7

    Available Since

    Nov. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. HpMscS

    Plasmid

    #78310

    Purpose

    H. pylori MscS with N-Term 6x Hisitidine Tag and C-Term FLAG Tag

    Depositor

    Douglas Rees

    Article

    Lai et al Protein Sci. 2013 Apr;22(4):502-9. doi:

    Insert

    Mechanosensitive channel of small conductance
    

    Tags

    6x His Tag and FLAG Tag

    Expression

    Bacterial

    Promoter

    T7 Promoter

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

    Plasmid

    #91873

    Purpose

    Photo-activatable/light sensitive Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Inserts

    LOV2
    VAV2 DH/PH/ZF (Vav2 Mouse)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUWR-CadTS

    Plasmid

    #58325

    Purpose

    Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

    Depositor

    Denise Montell

    Article

    Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

    Insert

    E-cadherin tension sensor
    

    Expression

    Insect

    Promoter

    poly-ubiquitin

    Available Since

    Aug. 26, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3.1 AT1AR-324delta

    Plasmid

    #45635

    Depositor

    Robert Lefkowitz

    Article

    Wei et al J Biol Chem. 2004 Nov 12;279(46):48255-6

    Insert

    AT1AR-324delta (Agtr1a Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    added stop codon at 324 aa

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

16. pUE1-TSP-ORF68 K395A/K396A

    Plasmid

    #162656

    Purpose

    Expresses ORF68 K395A/K396A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K395 and K396 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. As3 (pBW411J117-arsR)

    Plasmid

    #123142

    Purpose

    An arsenic sensor with optimized intracellular receptor density. Also used in the sensor cell array. pSB3K3 carrying J117-30arsR-t-ParsR-30gfp-t

    Depositor

    Baojun Wang

    Article

    Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

    Insert

    J117-30arsR-t-ParsR-30gfp-t
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J117, ParsR

    Available Since

    May 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Bxb1-GA-CpG-free-mStayGold

    Plasmid

    #241386

    Purpose

    Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of mStayGold with CpG-free promoter

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mStayGold
    

    Expression

    Mammalian

    Promoter

    CpG-free

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Vinculin-Clover-CC-S1-mRuby2

    Plasmid

    #233237

    Purpose

    Vinculin FRET tension sensor with coiled-coil S1 domain no vinculin force control

    Depositor

    Khalid Salaita

    Article

    Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

    Insert

    Vinculin-Clover-CC-S1-mRuby2
    

    Expression

    Mammalian

    Mutation

    Residues 1-228 of Clover, residues 1-3 deleted fr…

    Promoter

    CMV

    Available Since

    July 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEvol-chKlacRS-WT

    Plasmid

    #232159

    Purpose

    A chimeric PylRS for site-specific incorporation of L-lactyl-lysine into target proteins in E.coli cells.

    Depositor

    Nanxi Wang

    Article

    Shao et al Nat Commun. 2025 Jan 8;16(1):227. doi: 1

    Insert

    chKlacRS-WT
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge