We narrowed to 11,220 results for: NSI

1. pCfB2197-PrGNP1-mK2-NatMX

   Plasmid

   #216513

   Purpose

   Integrative fluorescent reporter for amino acid uptake sensing pathway, comprising mKate2 driven by the GNP1 promoter within the Easy clone vector, requires NotI digestion for integration in yeast

   Depositor

   Juha Saarikangas

   Article

   Paukstyte et al Mol Biol Cell. 2025 Apr 2:mbcE24040162.

   Insert

   PrGnp1-mKate2
   

   Use

   Cre/Lox; Integrative

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GNP1

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Vinculin-Clover-CC-S7-mRuby2

   Plasmid

   #233236

   Purpose

   Vinculin FRET tension sensor with coiled-coil S7 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S7-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Vinculin-Clover-CC-S4-mRuby2

   Plasmid

   #233235

   Purpose

   Vinculin FRET tension sensor with coiled-coil S4 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S4-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Vinculin-Clover-CC-S2-mRuby2

   Plasmid

   #233234

   Purpose

   Vinculin FRET tension sensor with coiled-coil S2 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S2-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pIVT-Bxb1-I87L, A369P, E434G

   Plasmid

   #232750

   Purpose

   IVT template for making modRNA encoding mutant Bxb1modRNA (I87L, A369P, E434G)

   Depositor

   Kate Galloway

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   Bxb1
   

   Use

   In vitro transcription

   Tags

   HA and SV40 NLS

   Expression

   Mutation

   I87L, A369P, E434G

   Promoter

   Available since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXJ-Myc-KLF16

   Plasmid

   #232655

   Purpose

   transient expresses KLF16 in mammalian cells

   Depositor

   Chen-Yang Shen

   Article

   Chou et al Cell Rep. 2024 Jan 23;43(1):113641. doi:

   Insert

   KLF16 (KLF16 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Bxb1-GT_CAG-ITPKA-mScarlet-I

   Plasmid

   #229778

   Purpose

   Bxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of a mScarlet-I F-actin reporter

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   mScarlet-I
   

   Use

   Synthetic Biology

   Tags

   ITPKA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Vinculin-Clover-CC-Mad-mRuby2

   Plasmid

   #233239

   Purpose

   Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-Mad-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Vinculin-Clover-CC-S0-mRuby2

   Plasmid

   #233233

   Purpose

   Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

   Depositor

   Khalid Salaita

   Article

   Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

   Insert

   Vinculin-Clover-CC-S0-mRuby2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Residues 1-228 of Clover, residues 1-3 deleted fr…

   Promoter

   CMV

   Available since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Bxb1-GA_AIO-TetOn_mScarlet_Convergent

    Plasmid

    #229790

    Purpose

    Bxb1-GA donor plasmid with convergent syntax for all-in-one doxycycline-inducible mScarlet expression

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    TRE-mScarlet; rtTA-T2A-mTagBFP2
    

    Use

    Synthetic Biology

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    TRE and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Bxb1-GT_hEF1a-mGL_CAG-mRuby2_Divergent

    Plasmid

    #229800

    Purpose

    Bxb1-GT donor plasmid with divergent syntax for constitutively expressing mRuby2 and mGreenLantern by the respective promoters CAG and hEF1a

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    mGreenLantern; mRuby2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hEF1a and CAG

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-22b(+) pCopA RBS30 sfgfp

    Plasmid

    #226371

    Purpose

    Plasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30

    Depositor

    Urartu Seker

    Article

    Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

    Insert

    sfGFP
    

    Use

    Tags

    6x HisTag

    Expression

    Bacterial

    Mutation

    Promoter

    copA promoter (pCopA) and RBS30 regulation

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hnRNPAB X2-IDR

    Plasmid

    #216517

    Purpose

    Expresses MBP-tagged intrinsically disordered region of hnRNPAB X2

    Depositor

    Nicolas Fawzi

    Article

    O'Connell et al Sci Rep. 2024 Nov 13;14(1):27890. doi: 1

    Insert

    hnRNPAB X2-IDR
    

    Use

    Tags

    6xHis tags-MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 2-225

    Promoter

    Available since

    Dec. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac SOX 318-320A

    Plasmid

    #192060

    Purpose

    Expression vector for SOX (ORF37) 318-320A

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (ORF37)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    318-320A

    Promoter

    Polyhedrin

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMDC Renilla Luc SRE

    Plasmid

    #191657

    Purpose

    Expressed Renilla Luciferase with SOX resistance element

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    Renilla Luciferase SRE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SOX D221A/E244A/K246A pACEmam1

    Plasmid

    #192050

    Purpose

    Expresses a 3X catalytic mutant of SOX (ORF37)

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (3X catalytic mutant)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    3X catalytic mutant D221A/E244A/K246A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pACE SOX (ORF37) K246A

    Plasmid

    #192051

    Purpose

    Expresses a catalytic mutant of SOX (K246A)

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (K246A)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Catalytic mutant K246A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pACE SOX (ORF37) K250A

    Plasmid

    #192052

    Purpose

    Expresses a DNA binding mutant K250A (ORF37)

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (K250A)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    DNA binding mutant K250A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pACE SOX (ORF37) 318-320A

    Plasmid

    #192054

    Purpose

    Expresses a mutant of SOX 318-320A

    Depositor

    Britt Glaunsinger

    Article

    Hartenian et al Nucleic Acids Res. 2023 Jan 11;51(1):182

    Insert

    SOX (318-320A)
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    SOX 318-320A

    Promoter

    CMV

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET26b_AT118-H-V5-His

    Plasmid

    #222850

    Purpose

    E. coli periplasmic expression plasmid encoding Nb. AT118-A, a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R)

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT118-H
    

    Use

    Tags

    V5-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge