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pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

Plasmid

#182348

Purpose

mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-Lifeact-mRuby

Plasmid

#194339

Purpose

Expresses Lifeact with mRuby which labels F-actin in mammalian cells and zebrafish embryos

Depositor

Heidi Hehnly

Article

Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

Insert

Lifeact

Tags

mRuby

Expression

Mammalian

Promoter

CMV IE94

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_m4_KRAB_mcherry

Plasmid

#167878

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pETM33_PDCD6IP_ALIX V-domain

Plasmid

#178483

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PDCD6IP_ALIX V-domain (PDCD6IP Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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NLS-HA-2xMCP-KRAB

Plasmid

#126589

Purpose

Expresses MCP (MS2 Coat Protein) fusion to KRAB in mammalian cells, lentiviral backbone

Depositor

David Segal

Article

O'Geen et al Epigenetics Chromatin. 2019 May 3;12(1):

Insert

2XMCP-KRAB

Use

Lentiviral

Tags

NLS-HA

Expression

Mammalian

Promoter

human ubiquitin C promoter

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM391 - mosTI unc-119 - Pmex-5 - NNN - tbb-2

Plasmid

#182351

Purpose

Germline expression cloning vector for mosTI(unc-119). Transgene inserted between Pmex-5 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Type

Empty backbone

Expression

Worm

Available Since

May 13, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-puro-N3FLAG-MYB-FL

Plasmid

#105596

Purpose

retrovirally express mouse MYB with 3*FLAG tag at N terminal and puro resistance

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

Full length MYB (mouse) (Myb Mouse)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Promoter

MSCV-LTR

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-NDUFAF5-HA

Plasmid

#86125

Purpose

Retroviral vector expressing NDUFAF5-HA-IRES-Bsd

Depositor

David Sabatini

Article

Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

Insert

NDUFAF5 (NDUFAF5 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAL188

Plasmid

#22277

Purpose

Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

Depositor

Christopher Voigt

Article

Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

Insert

TiamDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)

Use

Synthetic Biology

Expression

Mammalian

Promoter

SV40

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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pHR_PGK_SNIPR_hsNotch3 LNR

Plasmid

#188338

Purpose

Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a Notch3-ECD, a Notch3-TMD, a Notch3-JMD, and a Gal4VP64 transcriptional factor

Depositor

Kole Roybal

Article

Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

Insert

PGK_antiCD19_Notch3-ECD_Notch3-TMD_Notch3-JMD_Gal4VP64

Use

Lentiviral

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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